Strains of a new species of rapidly growing, nonphotochromogenic mycobacteria, , have been isolated from water, soil, and human sputum samples in Barcelona, Spain. The inclusion of this organism in the genus is based on its acid-alcohol fastness, its DNA G+C content, its mycolate pattern, and its mycolate pyrolysis esters. A study of 11 strains showed that they form a homogeneous group with an internal phenotypic similarity value of 94.9 ± 3.79%. The results of a comparison with 39 other mycobacterial species and subspecies are also presented. DNA relatedness studies showed that the strains studied form a single DNA hybridization group which is less than 30% related to 15 other species of the genus . Thin-layer chromatographic analysis showed that only α-mycolates are present. Unlike and α-mycolates, α-mycolates release only 22-carbon atom esters after pyrolysis. Strain CR-270 is the type strain; a culture of this strain has been deposited in the Collection Nationale de Cultures de Microorganismes de I'Institut Pasteur, Paris, France, as strain CIP 103465.


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