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Volume 43, Issue 2

Five New Species Isolated from Water Free

Abstract

Abstract

Fourteen like strains isolated from aquatic sources have been characterized serologically, biochemically, and in terms of DNA relatedness. The strains grew on buffered charcoal-yeast extract agar but not on blood agar and displayed phenotypic characteristics typical of the family , including a requirement for cysteine, cellular fatty acid compositions in which branched-chain acids predominate, and the possession of isoprenoid quinones of the ubiquinone series with more than 10 isoprene units in their side chains. All were nonfermentative, lacked urease, were incapable of nitrate reduction, and reacted positively with a DNA probe specific for the DNA hybridization studies in which the hydroxyapatite method was used demonstrated that the strains represented five new species of the genus Nine of the strains were more than 90% interrelated, and the name sp. nov. is proposed for this group. Two strains formed a second hybridization group, for which the name sp. nov. is proposed, while the three remaining species, sp. nov., sp. nov., and sp. nov., are each represented by a single strain. The levels of relatedness of the new species to each other are 23% or less, and the levels of relatedness to other members of the genus ranged from 0 to 36%. and are serologically unrelated to all other known species. cannot be separated from serogroup 4 by serological methods and is also serologically indistinguishable from ; distinctions may be made on the basis of fatty acid composition and biochemical reactions.

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Copyright 1993, International Union of Microbiological Societies
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1993-04-01
2024-04-19
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Five New Legionella Species Isolated from Water
Int J Syst Evol Microbiol 43, 329 (1993); https://doi.org/10.1099/00207713-43-2-329
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