1887

Abstract

Abstract

Eight actinomycete strains originally isolated from soil and plant samples were studied to determine their taxonomic status. All isolates produced branching substrate mycelia, but no distinct aerial hyphae. Relatively short chains of nonmotile spores (10 to 30 spores per chain) were borne on the tips of sporophores arising directly from the agar surface. The chemotaxonomic characteristics of the isolates, with the exception of the menaquinone profile, coincided with those of members of the family Goodfellow, Stanton, Simpson, and Minnikin 1990. Furthermore, the results of a phylogenetic analysis performed with 5S rRNA support the conclusion that the isolates should be classified in this family. The isolates differed from members of the constituent genera of the in morphological characteristics and menaquinone composition. Therefore, we propose a new genus for the strains, The type species and type strain are sp. nov. and strain K-319 (= JCM 8553), respectively.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-43-2-319
1993-04-01
2022-11-27
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/43/2/ijs-43-2-319.html?itemId=/content/journal/ijsem/10.1099/00207713-43-2-319&mimeType=html&fmt=ahah

References

  1. Athalye M., Goodfellow M., Minnikin D. E. 1984; Menaquinone composition in the classification of Actinomadura and related taxa. J. Gen. Microbiol. 130:817–823
    [Google Scholar]
  2. Collins M. D., Faulkner M., Keddie M. 1984; Menaquinone composition of some sporeforming actinomycetes. Syst. Appl. Microbiol. 5:20–29
    [Google Scholar]
  3. Collins M. D., Kroppenstedt R. M. 1987; Structures of the partially saturated menaquinones of Glycomyces rutgersensis. FEMS Microbiol. Lett. 44:215–219
    [Google Scholar]
  4. Collins M. D., Kroppenstedt R. M., Tamaoka J., Komagata K., Kinoshita T. 1988; Structures of tetrahydrogenated menaquinones from Actinomadura angiospora, Faenia rectivirgula, and Saccharothrix australiensis. Curr. Microbiol. 17:275–79
    [Google Scholar]
  5. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E. 1977; Distribution of menaquinones in actinomycetes and corynebacteria. J. Gen. Microbiol. 100:221–30
    [Google Scholar]
  6. Dekio S., Yamasaki R., Jidoi J., Hori H., Osawa S. 1984; Secondary structure and phylogeny of Staphylococcus and Micrococcus 5S rRNAs. J. Bacteriol. 159:233–37
    [Google Scholar]
  7. Donis-Keller H. 1980; Phy M: an RNase activity specific for U and A residues useful in RNA sequence analysis. Nucleic Acids Res. 8:3133–142
    [Google Scholar]
  8. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int. J. Syst. Bacteriol. 39:224–229
    [Google Scholar]
  9. Fowler V. J., Ludwig W., Stackebrandt E. 1985; Ribosomal ribonucleic acid cataloguing in bacterial systematics: the phylogeny of Actinomadura. 17–40 Goodfellow M., Minnikin D. E. Chemical methods in bacterial systematics Academic Press, Ltd.; London:
    [Google Scholar]
  10. Goodfellow M. 1971; Numerical taxonomy of some nocardio-form bacteria. J. Gen. Microbiol. 69:33–80
    [Google Scholar]
  11. Goodfellow M. 1989; The actinomycetes. I. Suprageneric classification of actinomycetes. 2333–2339 Williams S. T., Sharpe M. E., Holt and J. G. Bergey’s manual of systematic bacteriology 4 Williams & Wilkins; Baltimore:
    [Google Scholar]
  12. Goodfellow M., Cross T. 1984; Classification. 7–164 Goodfellow M., Mordarski M., Williams S. T. The biology of the actinomycetes Academic Press, Inc. (London), Ltd.; London:
    [Google Scholar]
  13. Goodfellow M., Stanton L. J., Simpson K. E., Minnikin D. E. 1990; Numerical and chemical classification of Actinoplanes and some related actinomycetes. J. Gen. Microbiol. 136:19–36
    [Google Scholar]
  14. Gordon R. E., Barnett D. A., Handerhan J. E., Pang C. H.-N. 1974; Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. Int. J. Syst. Bacteriol. 24:54–63
    [Google Scholar]
  15. Hayakawa M., Nonomura H. 1987; Humic acid-vitamin agar, a new medium for the selective isolation of soil actinomycetes. J. Ferment. Technol. 65:501–509
    [Google Scholar]
  16. Hori H., Osawa S. 1979; Evolutionary change in 5S RNA secondary structure and a phylogenic tree of 54 5S RNA species. Proc. Natl. Acad. Sci. USA 76:381–385
    [Google Scholar]
  17. Howarth O. W., Grand E., Kroppenstedt R. M., Collins M. D. 1986; Structural determination of a new naturally occurring cyclic vitamin K. Biochem. Biophys. Res. Commun. 140:916–923
    [Google Scholar]
  18. Jacobson E., Grauville W. C., Fogs C. E. 1958 Color harmony manual, 4th ed.. Container Corporation of America; Chicago:
    [Google Scholar]
  19. Johnson J. L. 1984; Bacterial classification. III. Nucleic acids in bacterial classification. 8–11 Krieg N. R., Holt J. G. Bergey’s manual of systematic bacteriology 1 Williams & Wilkins; Baltimore:
    [Google Scholar]
  20. Kaneko T., Katoh K., Fujimoto M., Kumagai M., Tamaoka J., Katayama-Fujimura Y. 1986; Determination of the nucleotide composition of a deoxyribonucleic acid by high-performance liquid chromatography of its enzymatic hydrolysate: a review. J. Microbiol. Methods 4:229–240
    [Google Scholar]
  21. Kawamoto I., Oka T., Nara T. 1981; Cell wall composition of Micromonospora olivoasterospora, Micromonospora sagamiensis, and related organisms. J. Bacteriol. 146:527–534
    [Google Scholar]
  22. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J. Mol. Evol. 16:111–120
    [Google Scholar]
  23. Kroppenstedt R. M. 1985; Fatty acid and menaquinone analysis of actinomycetes and related organisms. 173–199 Goodfellow M., Minnikin D. E. Chemical methods in bacterial systematics Academic Press, Ltd.; London:
    [Google Scholar]
  24. Kroppenstedt R. M. 1992; The genus Nocardiopsis. 1139–1156 Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K.-H. The prokaryotes Springer-Verlag New York, Inc.; New York:
    [Google Scholar]
  25. Kroppenstedt R. M., Stackebrandt E., Goodfellow M. 1990; Taxonomic revision of the actinomycete genera Actinomadura and Microtetraspora. Syst. Appl. Microbiol. 13:148–160
    [Google Scholar]
  26. Kudo T., Scino A. 1987; Transfer of Streptosporangium indianense Gupta 1965 to the genus Streptomyces as Streptomyces indiaensis (Gupta 1965) comb. nov. Int. J. Syst. Bacteriol. 37:241–244
    [Google Scholar]
  27. Lechevalier M. P., De Bievre C., Lechevalier H. 1977; Chemotaxonomy of aerobic actinomycetes: phospholipid composition. Biochem. Syst. Ecol. 5:249–260
    [Google Scholar]
  28. Lechevalier M. P., Lechevalier H. 1970; Chemical composition as a criterion in the classification of aerobic actinomycetes. Int. J. Syst. Bacteriol. 20:435–443
    [Google Scholar]
  29. Lechevalier M. P., Stern A. E., Lechevalier H. A. 1981; Phospholipids in the taxonomy of actinomycetes. Zentralbl. Bakteriol. Microbiol. Hyg. Abt. 1 Suppl. 11:111–116
    [Google Scholar]
  30. Meyertons J. L., Labeda D. P., Cote G. L., Lechevalier M. P. 1988; A new thin-layer chromatographic method for whole-cell sugar analysis of Micromonospora species. Actinomycetes 20:182–192
    [Google Scholar]
  31. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J. Microbiol. Methods 2:233–241
    [Google Scholar]
  32. Miyadoh S., Amano S., Tohyama H., Shomura T. 1990; A taxonomic review of the genus Microbispora and a proposal to transfer two species to the genus Actinomadura and to combine ten species into Microbispora rosea. J. Gen. Microbiol. 136:1905–1913
    [Google Scholar]
  33. Park Y.-H., Hori H., Suzuki K.-I., Osawa S., Komagata K. 1987; Phylogenetic analysis of the coryneform bacteria by 5 S rRNA sequences. J. Bacteriol. 169:1801–1806
    [Google Scholar]
  34. Park Y.-H., Yim D.-G., Kim E., Kho Y.-H., Mheen T.-I., Lonsdale J., Goodfellow M. 1991; Classification of acidophilic, neutrotolerant and neutrophilic streptomycetes by nucleotide sequencing of 5S ribosomal RNA. J. Gen. Microbiol. 137:2265–2269
    [Google Scholar]
  35. Peattie D. A. 1979; Direct chemical method for sequencing RNA. Proc. Natl. Acad. Sci. USA 76:1760–1764
    [Google Scholar]
  36. Saito H., Miura K. 1963; Preparation of transforming deoxyribonucleic acid by phenol treatment. Biochim. Biophys. Acta 72:619–629
    [Google Scholar]
  37. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4:406–425
    [Google Scholar]
  38. Sneath P. H. A., Sokal R. R. 1973 Numerical taxonomy W. H. Freeman & Co.; San Francisco:
    [Google Scholar]
  39. Staneck J. L., Roberts G. D. 1974; Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl. Microbiol. 28:226–231
    [Google Scholar]
  40. Stevenson I. L. 1967; Utilization of aromatic hydrocarbons by Arthrobacter spp. Can. J. Microbiol. 13:205–211
    [Google Scholar]
  41. Suzuki K., Komagata K. 1983; Taxonomic significance of cellular fatty acid composition in some coryneform bacteria. Int. J. Syst. Bacteriol. 33:188–200
    [Google Scholar]
  42. Tamaoka J., Katayama-Fujimura Y., Kuraishi H. 1983; Analysis of bacterial menaquinone mixtures by high performance liquid chromatography. J. Appl. Bacteriol. 54:31–36
    [Google Scholar]
  43. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol. Lett. 25:125–128
    [Google Scholar]
  44. Tomiyasu I. 1982; Mycolic acid composition and thermally adaptative changes in Nocardia asteroides. J. Bacteriol. 151:828–837
    [Google Scholar]
  45. Uchida K., Aida K. 1984; An improved method for the glycolate test for simple identification of the acyl type of bacterial cell walls. J. Gen. Appl. Microbiol. 30:131–134
    [Google Scholar]
  46. Yamada Y., Inouye G., Tahara Y., Kondo K. 1977; On the chemical structure of menaquinones with the tetrahydrogenated isoprenoid side chain. Biochim. Biophys. Acta 486:195–203
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-43-2-319
Loading
/content/journal/ijsem/10.1099/00207713-43-2-319
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error