1887

Abstract

Four strains of a novel species were isolated from the stomachs of cheetahs ( ) with gastritis. These isolates were phenotypically similar to The isolates were gram-negative, spiral bacteria which grew under microaerophilic conditions at 37°C, but not at 25 or 42°C, and produced urease, catalase, oxidase, alkaline phosphatase, and gamma-glutamyl transpeptidase. The isolates did not ferment glucose, mannitol, inositol, sorbitol, rhamnose, sucrose, melibiose, amygdalin, or arabinose; hydrolyze hippurate or indoxyl acetate; or reduce nitrate. They did not produce HS from triple sugar iron agar, and they did not grow in the presence of 1.0% glycine or 1.5% NaCl. They were resistant to nalidixic acid and sensitive to cephalothin and metronidazole. Cells were typically 0.3 by 2.0 μm and possessed tufts of two to five sheathed, monopolar flagella. The G+C content of strain 90-119 was 30 mol%. Cluster analysis of densitometry scans of polyacrylamide protein gels revealed more than 70% similarity of the cheetah isolates to , less than 60% similarity to and less than 50% similarity to Complete 16S rRNA sequences were determined for two of the cheetah isolates. Phylogenetic analysis was performed by comparing the cheetah sequences to those of 19 reference strains, inluding (two strains), subsp. , a sp. (pig isolate), , and The 16S rRNA sequences for 13 of the 19 reference species have not previously been reported. Phylogenetic analysis demonstrated that the cheetah isolates were most closely related to (97.4% similarity), (96.1% similarity), and (93.4% similarity). On the basis of these findings, we propose that these isolates represents a novel species of which we designate The type strain is 90-119 (CCUG 29263, ATCC 51101).

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1993-01-01
2024-03-02
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