1887

Abstract

Clustering and ordination were used to analyze the results of a study involving phenotypic characterization (61 tests) of 197 strains of gram-negative, oxidase-positive, rod-shaped bacteria isolated from carcharhinid sharks. Also included in the data set were data for 39 reference strains representing the genera , and . Unweighted pair group mathematical averaging cluster analysis resolved nine major groups of operational taxonomic units (OTUs) at simple matching coefficient (S) values of ≥66.5%. In turn, each group contained one or more OTU clusters (phena) at S values of ≥72.6%. A total of 27 phena were resolved; 14 phena were identified as species and contained 182 of the 236 OTUs in the study. Additional phena were identified as members of the genus (two phena), the genus (three phena), the genus (two phena), and (one phenon). One phenon resembled descriptions of the genera and and . The remaining four phena were not identified. The largest phena were identified as (52 OTUs) and (48 OTUs). and formed subclusters (S values, ≥82%) within the third largest phenon (24 OTUs), which was resolved at an S value of ≥72.6%. Human pathogens (e.g., ) and fish pathogens (e.g., , and were isolated from the sharks. Approximately 58% of all of the strains isolated and tested in this study were urease positive.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-43-1-88
1993-01-01
2024-06-18
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/43/1/ijs-43-1-88.html?itemId=/content/journal/ijsem/10.1099/00207713-43-1-88&mimeType=html&fmt=ahah

References

  1. Austin B., Priest F. 1986; Modern bacterial taxonomy Van Nostrand Reinhold (UK) Co., Ltd. Wokingham, Berkshire, England
    [Google Scholar]
  2. Baumann P., Baumann L. 1984; Genus II. Photobacterium Beijerinck 1889. p. 539–545 In Krieg N. R., Holt J. G. (ed.) Bergey’s manual of systematic bacteriology vol. 1 The Williams & Wilkins Co.; Baltimore:
    [Google Scholar]
  3. Baumann P., Fumiss A. L., Lee J. V. 1984; Genus I.Vibrio Pacini 1854. p. 518–538 In Krieg N. R., Holt J. G. (ed.) Bergey’s manual of systematic bacteriology vol. 1: The Williams & Wilkins Co; Baltimore:
    [Google Scholar]
  4. Baumann P., Gauthier M. J., Baumann L. 1984; Genus Alteromonas Baumann, Baumann, Mandel and Allen 1972. p. 343–352 In Krieg N. R., Holt J. G. (ed.) Bergey’s manual of systematic bacteriology vol. 1 The Williams & Wilkins Co.; Baltimore:
    [Google Scholar]
  5. Beaver J. L., Grimes D. J., Colwell R. R. Unpublished data
  6. Bochner B. (Biolog, Inc., Hayward, Calif.) Personal communication
    [Google Scholar]
  7. Carter G. R. 1984; Genus I.Pasteurella Trevisan 1887. p. 552–558 In Krieg N. R., Holt J. G. (ed.) Bergey’s manual of systematic bacteriology vol. 1 The Williams & Wilkins Co; Baltimore:
    [Google Scholar]
  8. Cheung P. J., Ruggieri G. D. 1983; Dermophthirius nigrellii n. sp. (Monogenea: Microbothriidae), an ectoparasite from the skin of the lemon shark, Negaprion brevirostris. Trans. Am. Microsc. Soc 102:129–134
    [Google Scholar]
  9. Grimes D. J. 19901; Review of human pathogenic bacteria in marine animals with emphasis on sharks. p. 63–69 In Pratt H. L. Jr., Gruber S. H., Taniuchi T. (ed.) Elasmobranchs as living resources: advances in the biology, ecology, system- atics, and the status of the fisheries. National Oceanic and Atmospheric Administration Technical Report 90 U.S. Department of Commerce; Washington, D.C:
    [Google Scholar]
  10. Grimes D. J., Brayton P., Colwell R. R., Gruber S. H. 1985; Vibrios as autochthonous flora of neritic sharks. Syst. Appl. Microbiol 6:221–226
    [Google Scholar]
  11. Grimes D. J., Gruber S. H., May E. B. 1985; Experimental infection of lemon sharks, Negaprion brevirostris (Poey), with Vibrio species. J. Fish Dis 8:173–180
    [Google Scholar]
  12. Grimes D. J., Stemmier J., Hada H., May E. B., Maneval D., Hetrick F. M., Jones R. T., Stoskopf M., Colwell R. R. 1984; Vibrio species associated with mortality of sharks held in captivity. Microb. Ecol 10:271–282
    [Google Scholar]
  13. Jacobs D. 1990; SAS/GRAPH software and numerical taxonomy. p. 1413–1418 In Proceedings of the 15th Annual SAS Users Group International Conference SAS Institute, Inc.; Cary, N.C:
    [Google Scholar]
  14. Janssen W. A., Surgalla M. J. 1968; Morphology, physiology, and serology of a Pasteurella species pathogenic for white perch (Roccus americanus ). J. Bacteriol 96:1606–1610
    [Google Scholar]
  15. Kaper J. B., Lockman H., Remmers E. F., Kristensen K., Colwell R. R. 1983; Numerical taxonomy of vibrios isolated from estuarine environments. Int. J. Syst. Bacteriol 33:229–255
    [Google Scholar]
  16. Kita-Tsukamoto K., Oyaizu H., Nanba K., Simidu U. 1993; Phylogenetic relationships of marine bacteria, mainly members of the family Vibrionaceae, determined on the basis of 16S rRNA sequences. Int. J. Syst. Bacteriol 43:8–19
    [Google Scholar]
  17. Knight I. T., Grimes D. J., Colwell R. R. 1988; Bacterial hydrolysis of urea in the tissues of carcharhinid sharks. Can. J. Fish. Aquat. Sci 45:357–360
    [Google Scholar]
  18. Krichevsky M. I., Walczak A. 1982; Computer-aided selection of group descriptors as exemplified by data on Cap- nocytophaga species. Curr. Microbiol 7:199–204
    [Google Scholar]
  19. Love M., Teebken-Fisher D., Hose J. E., III Farmer J. J., Hickmanl F. W., Fanning G. R. 1981; Vibrio damsela, a marine bacterium, causes skin ulcers on the damselfish, Chromis punc- tipinnis. Science 214:1139–1140
    [Google Scholar]
  20. MacDonell M. T., Colwell R. R. 1985; Phylogeny of the Vibrionaceae, and recommendation for two new genera, Lis- tonella and Shewanella. Syst. Appl. Microbiol 6:171–182
    [Google Scholar]
  21. MacDonell M. T., Swartz D. G., Ortiz-Conde B. A., Last G. A., Colwell R. R. 1986; Ribosomal RNA phylogenies for the vibrio-enteric group of eubacteria. Microbiol. Sci 3:172–178
    [Google Scholar]
  22. Pielou E. C. 1984; The interpretation of ecological data. John Wiley & Sons, Inc; New York:
    [Google Scholar]
  23. Pillidge C. J., Colwell R. R. 1988; Nucleotide sequence of the 5S rRNA from Listonella (Vibrio ) ordalii ATCC 33509 and Listonella (Vibrio ) tubiashii ATCC 19105. Nucleic Acids Res 16:3111
    [Google Scholar]
  24. Pillidge C. J., MacDonell M. T., Colwell R. R. 1987; Nucleotide sequence of the 5S rRNA from Listonella (Vibrio) aestuarianus ATCC 35048. Nucleic Acids Res 15:1879
    [Google Scholar]
  25. Popoff M. 1984; Genus III. Aeromonas Kluyver and Van Niel 1936. p. 545–548 In Krieg N. R., Holt J. G. (ed.) Bergey’s manual of systematic bacteriology vol. 1 The Williams & Wilkins Co.; Baltimore:
    [Google Scholar]
  26. Simidu U., Tsukamoto K. 1985; Habitat segregation and biochemical activities of marine members of the family Vibrion-aceae. Appl. Environ. Microbiol 50:781–790
    [Google Scholar]
  27. Singleton F. L. Personal communication
  28. Smith S. K., Sutton D. C., Fuerst J. A., Reichelt J. L. 1991; Evaluation of the genus Listonella and reassignment of Listonella damsela (Love et al.)MacDonell and Colwell to the genus Photobacterium as Photobacterium damsela comb. nov. with an emended description. Int. J. Syst. Bacteriol 41:529–534
    [Google Scholar]
  29. Sneath P. H. A., Sokal R. R. 1973; Numerical taxonomy. W. H. Freeman and Co.; San Francisco:
    [Google Scholar]
  30. Snieszko S. F., Bullock G. L., Hollis E., Boone J. G. 1964; Pasteurella sp. from an epizootic of white perch (Roccus americanus) in Chesapeake Bay tidewater areas. J. Bacteriol 88:1814–1815
    [Google Scholar]
  31. West P. A., Brayton P. R., Bryant T. N., Colwell R. R. 1986; Numerical taxonomy of vibrios isolated from aquatic environments. Int. J. Syst. Bacteriol 36:531–543
    [Google Scholar]
  32. West P. A., Brayton P. R., Twilley R. R., Bryant T. N., Colwell R. R. 1985; Numerical taxonomy of nitrogen-fixing “decarboxylase-negative” Vibrio species isolated from aquatic environments. Int. J. Syst. Bacteriol 35:198–205
    [Google Scholar]
  33. West P. A., Colwell R. R. 1984; Identification and classification of Vibrionaceae —an overview. p. 285–363 In Colwell R. R. (ed.) Vibrios in the environment John Wiley & Sons; New York:
    [Google Scholar]
  34. West P. A., Lee J. V., Bryant T. N. 1983; A numerical taxonomic study of species of Vibrio isolated from the aquatic environment and birds in Kent, England. J. Appl. Bacteriol 55:263–282
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-43-1-88
Loading
/content/journal/ijsem/10.1099/00207713-43-1-88
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error