@article{mbs:/content/journal/ijsem/10.1099/00207713-43-1-77, author = "Dobson, S. J. and Colwell, R. R. and Mcmeekin, T. A. and Franzmann, P. D.", title = "Direct Sequencing of the Polymerase Chain Reaction-Amplified 16S rRNA Gene of Flavobacterium gondwanense sp. nov. and Flavobacterium salegens sp. nov., Two New Species from a Hypersaline Antarctic Lake", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "1993", volume = "43", number = "1", pages = "77-83", doi = "https://doi.org/10.1099/00207713-43-1-77", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/00207713-43-1-77", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = "Phenotypic data and phospholipid ester-linked fatty acid profiles indicate that pigmented bacterial strains isolated from a hypersaline Antarctic lake are members of the “flavobacterium-bacteroides” phylum and may represent new taxa. Nearly complete 16S rRNA sequences were obtained for representative strains by directly sequencing the polymerase chain reaction-amplified 16S rRNA gene. Sequence signatures confirmed that these organisms were members of the flavobacterium-bacteroides phylum. A phylogenetic analysis, in which the sequences of the Antarctic strains were compared with a large number of sequences available for members of the flavobacterium-bacteroides phylum, showed that the Antarctic strains were phylogenetically distinct. The new species cluster with a group of organisms that contains the type species of the genus Flavobacterium, Flavobacterium aquatile. Two new species are described, for which the names Flavobacterium gondwanense and Flavobacterium salegens are proposed; strains ACAM 44 (= DSM 5423) and ACAM 48 (= DSM 5424) are the type strains of F. gondwanense and F. salegens, respectively.", }