RT Journal Article SR Electronic(1) A1 Ochiai, Keiko A1 Uchida, Kazuhisa A1 Kawamoto, IsaoYR 1993 T1 Similarity of Ribosomal Proteins Studied by Two-Dimensional Coelectrophoresis for Identification of Gram-Positive Bacteria JF International Journal of Systematic and Evolutionary Microbiology, VO 43 IS 1 SP 69 OP 76 DO https://doi.org/10.1099/00207713-43-1-69 PB Microbiology Society, SN 1466-5034, AB A two-dimensional electrophoresis method was devised for comparing ribosomal proteins from different bacteria. First-dimension separation of the proteins was carried out by nonequilibrium pH gradient gel electrophoresis in glass capillary tubes. Next, the first-dimension gels were developed in a slab gel by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Within about 3 h (total running time), 40 to 48 distinct and compact ribosomal protein spots were detected in each of the 34 strains belonging to eight genera of gram-positive bacteria which we studied. Similarity coefficients (SAB values) among the proteins of Corynebacterium and Arthrobacter strains were calculated by determining the numbers of coincident spots obtained by coelectrophoresis. The SAB values between strains having DNA relatedness values of more than 61% (strains of the same species) were greater than 0.85, whereas the SAB values between strains of different species were less than 0.45. Ribosomal protein analysis by our improved method is a potential tool for species identification of bacteria., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/00207713-43-1-69