1887

Abstract

A recently isolated, selenate-respiring microorganism (strain AX [T = type strain]) was classified by using a polyphasic approach in which both genotypic and phenotypic characteristics were determined. Strain AX is a motile, gram-negative, rod-shaped organism with a singlepolar flagellum. On the basis of phenotypic characteristics, this organism can be classified as a sp. However, a comparison of the 16S rRNA sequence of strain AX with the sequences of other organisms indicated that strain AX is most similar to members of the beta subclass (level of similarity, 86.8%) rather than to members of the gamma subclass (level of similarity, 80.2%) of the . The presence of the specific polyamine 2-hydroxyputrescine and the presence of a ubiquinone with eight isoprenoid units in the side chain (ubiquinone Q-8) excluded strain AX from the authentic genus and allowed placement in the beta subclass of the . Within the beta subclass, strain AX is related to . The phylogenetic distance (level of similarity, less than 90%), as well as a lack of common phenotypic characteristics between these organisms, prevents classification of strain AX as a member of the genus . In addition, strain AX possesses a unique mechanism for anaerobic respiration, which allows it to utilize selenate as an electron acceptor without interference by nitrate. Therefore, we propose that strain AX should be the first member of a new genus and species, .

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1993-01-01
2022-05-20
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