1887

Abstract

A mollicutes isolated from a guinea pig vagina was shown to be serologically distinct from previously recognized and species. Colonies isolated from 10 different guinea pigs were cloned and examined in detail. These strains were closely related and had the following properties: Guanine-plus-cytosine content of 36 mol%, no requirement for sterol, and aerobic growth. Glucose was not metabolized, and arginine and urea were not hydrolyzed. Strain GP3 (= NCTC 11727) is the type strain of a new species,

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-42-4-589
1992-10-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/42/4/ijs-42-4-589.html?itemId=/content/journal/ijsem/10.1099/00207713-42-4-589&mimeType=html&fmt=ahah

References

  1. Aluotto B. B., Wittier R. G., Williams C. O., Faber J. E. 1970; Standardized bacteriologic techniques for the characterization of Mycoplasma species. Int. J. Syst. Bacteriol. 20:35–58
    [Google Scholar]
  2. Barber T. L., Fabricant J. 1971; Identification of Mycoplasmatales: characterization procedures. Appl. Microbiol. 21:600–605
    [Google Scholar]
  3. Boatman E. S. 1979 Morphology and ultrastructure of the Mycoplasmatales,. 63–102 Barile M. F., Razin S.ed The Mycoplasmas 1 Academic Press, Inc.; New York:
    [Google Scholar]
  4. Clyde W. A. Jr. 1964; Mycoplasma species identification based upon growth inhibition by specific antisera. J. Immunol. 92:958–965
    [Google Scholar]
  5. Edward D. G. ff. 1971; Determination of sterol requirement for Mycoplasmatales. J. Gen. Microbiol. 69:205–210
    [Google Scholar]
  6. Edward D. G. ff., Fitzgerald W. A. 1951; Cholesterol in the growth of organisms of the pleuropneumonia group. J. Gen. Microbiol. 5:576–586
    [Google Scholar]
  7. Fabricant J., Freundt E. A. 1967; Importance of extension and standardization of laboratory tests for the identification and classification of mycoplasma. Ann. N.Y. Acad. Sci. 143:50–58
    [Google Scholar]
  8. Fallon R. J., Whittlestone P. 1969 Isolation, cultivation and maintenance of mycoplasmas. 211–267 Norris J. R., Ribbins D. W.ed Methods in microbiology 3B Academic Press, Inc.; New York:
    [Google Scholar]
  9. Freundt E. A., Andrews B. E., Ernø H., Kunze M., Black F. T. 1973; The sensitivity of Mycoplasmatales to sodium-polyanethol sulfonate and digitonin. Zentralbl. Bakteriol. Parasitenkd. Infectionskr. Hyg. Abt. 1 Orig. Reihe A 225:104–112
    [Google Scholar]
  10. Gross-Bellard M. J., Oudét P., Chambon P. 1973; Isolation of high molecular weight DNA from mammalian cells. Eur. J. Biochem. 36:32–38
    [Google Scholar]
  11. Hill A. C. 1971; Mycoplasma caviae, a new species. J. Gen. Microbiol. 65:109–113
    [Google Scholar]
  12. Hill A. C. 1971; The isolation of two further species of mycoplasma from guinea pigs. Vet. Rec. 83:225
    [Google Scholar]
  13. Hill A. C. 1974 Mycoplasmas of small animal hosts. 311–316 Bove J. M., Duplan J. F.ed Les Mycoplasmes/ Mycoplasmas 33 Institut National de la Sante et de la Recherche Medicale; Paris, France:
    [Google Scholar]
  14. Hill A. C. 1977; The metabolic inhibition test for mycoplasmas based on phosphatase production. J. Hyg. 79:391–393
    [Google Scholar]
  15. Hill A. C. 1984; Mycoplasma cavipharyngis, a new species isolated from the nasopharynx of guinea-pigs. J. Gen. Microbiol. 130:3183–3188
    [Google Scholar]
  16. Klieneberger-Nobel E. 1962 Morphology of pleuropneumonialike organisms. 23–56 Klieneberger-Nobel E.ed Pleuropneumonia-like organisms (PPLO). Mycoplasmataceae Academic Press, Inc.; New York:
    [Google Scholar]
  17. Leach R. H. 1976; The inhibitory effect of arginine on growth of some mycoplasmas. J. Appl. Bacteriol. 41:259–264
    [Google Scholar]
  18. Manchee R. J., Taylor-Robinson D. 1968; Hemadsorption and hemagglutination by mycoplasmas. J. Gen. Microbiol. 50:465–478
    [Google Scholar]
  19. Morton H. E., Roberts R. J. 1967; Production of antimycoplasma (PPLO) antibodies in rabbits. Proc. Soc. Exp. Biol. Med. 125:538–543
    [Google Scholar]
  20. Mouches C., Bové J. M. 1983; Electrophoretic characterization of mycoplasma proteins. Methods Mycoplasmol. 1:241–255
    [Google Scholar]
  21. Polak-Vogelzang A. A., Hagenaars R., Nagel S. 1978; Evaluation of an indirect immunoperoxidase test for identification of Acholeplasma and Mycoplasma. J. Gen. Microbiol. 106:241–249
    [Google Scholar]
  22. Purcell R. H., Taylor-Robinson D., Wong D. C., Chanock R. M. 1966; A color test for the measurement of antibody to the non-acid-forming human mycoplasma species. Am. J. Epidemiol. 84:51–66
    [Google Scholar]
  23. Purcell R. H., Taylor-Robinson D., Wong D. C., Chanock R. M. 1966; Color test for the measurement of antibody to T-strain mycoplasmas. J. Bacteriol. 92:6–12
    [Google Scholar]
  24. Razin S., Tully J. G. 1970; Cholesterol requirement of mycoplasmas. J. Bacteriol. 102:306–310
    [Google Scholar]
  25. Schildkraut C. L., Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its buoyant density in CsCl. J. Mol. Biol. 4:430–443
    [Google Scholar]
  26. Shepard M. C., Howard D. R. 1970; Identification of “T” mycoplasmas in primary agar cultures by means of a direct test for urease. Ann. N.Y. Acad. Sci. 174:809–819
    [Google Scholar]
  27. Subcommittee on the Taxonomy of Mollicutes 1979; Proposal of minimal standards for descriptions of new species of the class Mollicutes. Int. J. Syst. Bacteriol. 29:172–180
    [Google Scholar]
  28. Taylor-Robinson D., Purcell R. H., Wong D. C., Chanock R. M. 1966; A colour test for the measurement of antibody to certain mycoplasma species based upon the inhibition of acid production. J. Hyg. 64:91–104
    [Google Scholar]
  29. Taylor-Robinson D., Williams M. H., Haig D. A. 1968; The isolation and comparative biological and physical characteristics of T-mycoplasmas of cattle. J. Gen. Microbiol. 54:33–46
    [Google Scholar]
  30. Timms L. 1967; Isolation and identification of avian mycoplasmas. J. Med. Lab. Technol. 24:79–89
    [Google Scholar]
  31. Tully J. G. 1965; Biochemical, morphological and serological characteristics of mycoplasma of murine origin. J. Infect. Dis. 115:171–185
    [Google Scholar]
  32. Tully J. G., Razin S. 1969; Characteristics of a new sterol-nonrequiring Mycqp/asma. J. Bacteriol. 98:970–978
    [Google Scholar]
  33. Venable J. H., Coggeshall R. 1965; A simplified lead citrate stain for use in electron microscopy. J. Cell Biol. 25:407
    [Google Scholar]
  34. Williams C. O., Wittier R. G. 1971; Hydrolysis of esculin and phosphatase production by members of the order Mycoplasmatales which do not require sterol. Int. J. Syst. Bacteriol. 21:73–77
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-42-4-589
Loading
/content/journal/ijsem/10.1099/00207713-42-4-589
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error