On the basis of phenotypical characteristics and analysis of 16S rRNA sequence, a new species belonging to a new genus is described, and the name Marinobacter hydrocarbonoclasticus is proposed. This organism, isolated from Mediterranean seawater near a petroleum refinery, is a gram-negative, aerobic, rod-shaped bacterium. It grows at NaCl concentrations of 0.08 to 3.5 M and uses various hydrocarbons as the sole source of carbon and energy. Its DNA has a guanine-plus-cytosine content of 52.7 mol%. The 16S rRNA analysis shows a clear affiliation between M. hydrocarbonoclasticus and the gamma group of the phylum Proteobacteria. A close phylogenetic relationship appears among the species Marinomonas vaga, Oceanospirillum linum, Halomonas elongata, and Pseudomonas aeruginosa. Because of the impossibility of finding a single most closely related species, we suggest that this bacterium be assigned to a new genus, at least temporarily. The possibility of a revision of this status when new data appear is, however, not excluded. The type strain is M. hydrocarbonoclasticus SP.17 (=ATCC 49840).
Al-MallahM., GoutxM., MilleG., BertrandJ. C.1990; Production of emulsifying agents during growth of a marine Alteromonas in seawater with eicosane as carbon source, a solid hydrocarbon. Oil Chern. Pollut. 6:289–305
BaumannP., GauthierM. J., BaumannL.1984 Genus Alteromonas Baumann, Baumann, Mandel and Allen, 1972, 418. 243–352KriegN. R., HoltJ. G.edBergey’s manual of systematic bacteriology1 The Williams & Wilkins Co.; Baltimore:
BertrandJ. C., BazinH., AzoulayE.1976 Isolement et étude d’une bactérie marine se développant sur hydrocarbures. II. Etude de la lyse et de la viabilité. Ann. Microbiol Institut Pasteur; 127B393–409
BertrandJ. C., Al-MallahM., AcquavivaM., MilleG.1990; Biodegradation of hydrocarbons by an extremely halophilic archaebacterium. Lett. Appl. Microbiol. 11:260–263
ChanY. K., KnowlesR.1979; Measurement of denitrification in freshwater sediments by an in situ acetylene inhibition method. Appl. Environ. Microbiol. 37:1067–1072
CostertonJ. W., FosbergC., MatulaT. I., BuckmireF. L. A., McLeodR. A.1967; Nutrition and metabolism of marine bacteria. XVI. Formation of protoplasts, spheroplasts, and related forms from a Gram-negative marine bacterium. J. Bacteriol. 94:1764–1777
DenisM., RichaudP.1982; Dynamics of carbon monoxide recombination to fully reduced cytochrome c oxidase in plant mitochondria after low-temperature flash photolysis. Biochem. J. 206:379–385
De VoeI. W., OginskyE. L.1969; Antagonistic effect of monovalent cations in maintenance of cellular integrity of a marine bacterium. J. Bacteriol. 98:1355–1367
GauthierM. J., BreittmayerV. A.1992 The genera Alteromonas and Marinomonas,. 3046–3070BalowsA., TrüperH. G., DworkinM., HarderW., SchleiferK.-H.edThe prokaryotes3 Springer-Verlag; New York:
JahnK.1986 Isolation and characterisation of fimbriae from Escherichia coli,. 381–388SussmanM.edThe virulence of Escherichia coli. Reviews and methods. The Society for General Microbiology Academic Press; London:
JohnsonJ. L.1981 Genetic characterization. 450–472GerhardtP., MurrayR. G. E., CostilowR. N., NesterE. W., WoodW. A., KriegN. R., PhillipsG. B.edManual of methods for general bacteriology American Society for Microbiology; Washington, D.C:
KerstersK., De LeyJ.1984 Genus Alcaligenes Castellani and Chalmers 1919, 936AL. 361–373KriegN. R., HoltJ. G.edBergey’s manual of systematic bacteriology1 The Williams & Wilkins Co.; Baltimore:
KhannaG., De VoeL., BrownL., NivenD. F., McLeodR. A.1984; Relationship between ion requirements for respiration and membrane transport in a marine bacterium. J. Bacteriol. 157:59–63
KriegN. R.1984 Genus Oceanospirillum Hylemon, Wells, Krieg and Jannasch 1973, 361AL. 104–110KriegN. R., HoltJ. G.edBergey’s manual of systematic bacteriology1 The Williams & Wilkins Co.; Baltimore:
QuL. H., MichotB., BachellerieJ. P.1983; Improved method for structure probing in large RNAs: a rapid heterologous sequencing approach is coupled to the direct mapping of nuclease accessible sites. Application to the 5′ terminal domain of eukaryotic 28S rRNA. Nucleic Acids Res. 11:5903–5920
RaymanM. K., McLeodR. A.1975; Interaction of Mg2+with peptidoglycan and its relation to the prevention of lysis of a marine pseudomonad. J. Bacteriol. 122:650–659
SmibertR. M., KriegN. R.1981 General characterization. 409–443GerhardtP., MurrayR. G. E., CostilowR. N., NesterE. W., WoodW. A., KriegN. R., PhillipsG. B.edManual of methods for general bacteriology American Society for Microbiology; Washington, D.C:
SmithA. B., LafayB., ChristenR. Comparative variation of morphological and molecular evolution through geologic time: 28S ribosomal RNA versus morphology in echinoids. Philos. Trans. Royal Soc. London B in press
StackebrandtE.1988; Phylogenetic relationships vs. phenotypic diversity: how to achieve a phylogenetic classification of the eubacteria. Can. J. Microbiol. 34:552–556
StackebrandtE., MurrayR. G. E., TrüperH. G.1988; Proteobacteria classis nov., a name for the phylogenetic taxon that includes the “purple bacteria and their relatives.”. Int. J. Syst. Bacteriol. 38:321–325
StevensonP. M., RuettingerR. T., FulcoA. J.1983; Cytochrome P-450 revealed: the effect of the respiratory cytochromes on the spectrum of bacterial cytochrome P-450. Biochem. Biophys. Res. Commun. 112:927–934
SwoffordD.1990 PAUP: phylogenetic analysis using parsimony, version 3.0. Computer program distributed by the Illinois Natural History Survey; Champaign:
TiedjeJ. M.1988 Ecology of denitrification and dissimilatory nitrate reduction to ammonium. 179–244ZehnderA. J. B.edBiology of anaerobic microorganisms, tome I John Wiley & Sons, Inc.; New York:
Van de PeerY., NeefsJ., De WachterR.1990; Small ribosomal subunit RNA sequences, evolutionary relationships among different life forms, and mitochondrial origins. J. Mol. Evol. 30:463–476
VreelandR. H.1984 Genus Halomonas Vreeland, Litchfield, Martin and Elliot, 1980, 494. 340–343KriegN. R., HoltJ. G.edBergey’s manual of systematic bacteriology1 The Williams & Wilkins Co.; Baltimore:
VreelandR. H., LitchfieldC. D., MartinE. L., ElliotE.1980; Halomonas elongata, gen. nov.: a previously uncharacterized bacterial genus isolated from a Caribbean solar salt facility. Int. J. Syst. Bacteriol. 30:485–495
VreelandR. H., MatinE. L.1980; Growth characteristics, effects of temperature, and ion specificity of the halo tolerant bacterium Halomonas elongata. Can. J. Microbiol. 26:746–752
WoeseC. R., KandlerO., WheelisM. L.1990; Towards a natural system of organisms: proposal for the domains Archaea, Bacteria and Eucarya. Proc. Natl. Acad. Sci. USA 87:4576–4579