1887

Abstract

A new species of rapidly growing, nonphotochromogenic mycobacteria, , is described. The inclusion of this organism in the genus is based on its acid fastness, its mycolate pattern, and its G+C content. A study of six strains showed that they form a homogeneous group with an internal phenotypic similarity value of 97 ± 2.22%. DNA relatedness studies showed that the six strains which we studied form a single DNA hybridization group which is less than 49% related to 14 other species of the genus the δTm values determined for the strains which exhibited higher levels of DNA homology were all greater than 7.9°C. A Iipid analysis showed that tuberculostearic acid was present. Docosanoic and tetracosanoic acid methyl esters were detected as mycolic acid cleavage products. All six isolates which we tested contained α-mycolic acids and relatively large amounts of a new kind of mycolic acid containing a methoxy group at ω-1 position, a characteristic that has not been described previously in mycobacteria. Strain CR-21 is the type strain; a culture of this strain has been deposited in the Collection Nationale de Cultures de Microorganismes de I’Institut Pasteur, Paris, France, as strain CIP 103464.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-42-4-529
1992-10-01
2024-07-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/42/4/ijs-42-4-529.html?itemId=/content/journal/ijsem/10.1099/00207713-42-4-529&mimeType=html&fmt=ahah

References

  1. Brennan P. J. 1988 Mycobacterium and other actinomycetes. 203–298 Ratledge C., Wilkinson S. G.ed Microbial lipids Academic Press; London:
    [Google Scholar]
  2. Canetti G., Rist N., Grosset J. 1963; Mesure de la sensibilité du bacille tuberculeux aux drogues antibacillaires par la méthode des proportions. Rev. Tuberc. Pneumol. 27:217–272
    [Google Scholar]
  3. Crosa J. H., Brenner D. J., Falkow S. 1973; Use of a single-strand-specific nuclease for analysis of bacterial and plasmid deoxyribonucleic acid homo- and heteroduplexes. J. Bacteriol. 115:904–911
    [Google Scholar]
  4. Daffé M., Lanéelle M. A., Asselineau C., Lévy-Frébault V., David H. 1983; Intérêt taxonomique des acides gras des mycobactéries: proposition d’une méthode d’analyse. Ann. Inst. Pasteur Microbiol 134B:241–256
    [Google Scholar]
  5. Daffé M., Lanéelle M. A., Promé D., Asselineau C. 1981; Étude des lípides de Mycobacterium gordonae comparativement a ceux de M. leprae et des quelques mycobactéries scotocromogénes. Ann. Inst. Pasteur Microbiol 132B:3–12
    [Google Scholar]
  6. Daffé M., Lanéelle M. A., Puzo G., Asselineau C. 1981; Acide mycolique époxydique: un nouveau type d’acide mycolique. Tetrahedron Lett. 22:4515–4516
    [Google Scholar]
  7. David H. L., Jahan M. T. 1977; β-Glucosidase activity in mycobacteria. J. Clin. Microbiol. 13:6–9
    [Google Scholar]
  8. David H. L., Lévy-Frébault V., Thorel M. F. 1989 Méthodes de laboratoire pour mycobactériologie clinique. Instituí Pasteur; Paris:
    [Google Scholar]
  9. David H. L., Traore I., Feuillet A. 1981; Differential identification of Mycobacterium fortuitum and Mycobacterium chelonei. J. Clin. Microbiol. 5:383–384
    [Google Scholar]
  10. Dobson G., Minnikin D. E., Minnikin S. M., Parlett J. H., Goodfellow M., Ridell M., Magnusson M. 1985 Systematic analysis of complex mycobacterial lipids. 237–265 Goodfellow M., Minnikin D. E.ed Chemical methods in bacterial systematics Academic Press, Inc. (London), Ltd.; London:
    [Google Scholar]
  11. Goodfellow M. E., Minnikin D. E. 1984 Circumscription of the genus. 1–24 Kubica G. P., Wayne L. G.ed The mycobacteria. A sourcebook, part A Marcel Dekker, Inc.; New York:
    [Google Scholar]
  12. Goodfellow M. E., Orlean P. A. B., Collins M. D., Alshamoany L., Minnikin D. E. 1978; Chemical and numerical taxonomy of strains received as Gordona aurantiaca. J. Gen. Microbiol. 109:57–68
    [Google Scholar]
  13. Grimont P. A. D., Popoff M. Y., Grimont F., Coynault C., Lemelin M. 1980; Reproducibility and correlation study of three deoxyribonucleic acid hybridization procedures. Curr. Microbiol. 4:325–330
    [Google Scholar]
  14. Kazda J., Stackebrandt E., Smida J., Minnikin D. E., Daffé M., Parlett J. H., Pitulle C. 1990; Mycobacterium cookii sp. nov. Int. J. Syst. Bacteriol. 40:217–223
    [Google Scholar]
  15. Kubica G. P., David H. L. 1980 The mycobacteria. 1693–1730 Sonnenwirth A. C., Jarett L.ed Gradwohl’s clinical laboratory methods and diagnosis The C. V. Mosby Co.; St. Louis:
    [Google Scholar]
  16. Kubica G. P., Good R. C. 1981 The genus Mycobacterium,. 1962–1984 Starr M. P., Stolp H., Trüper H. G., Balows A., Schlegel H. G.ed The prokaryotes: a handbook on habitats, isolation and identification of bacteria SpringerVerlag; Berlin:
    [Google Scholar]
  17. Kubica G. P., Pool G. L. 1960; Studies on the catalase activity of acid fast bacilli. I. An attempt to subgroup these organisms on the basis of their catalase activities at different temperatures and pH. Am. Rev. Respir. Dis. 81:387–391
    [Google Scholar]
  18. Lambert M. A., Moss C. W., Silcox V. A., Good R. 1986; Analysis of mycolic acid cleveage products and cellular fatty acids of Mycobacterium species by capillary gas chromatography. J. Clin. Microbiol. 23:731–736
    [Google Scholar]
  19. Lanéelle M. A., Lacave C., Daffé M., Lanéelle G. 1988; Mycolic acids of Mycobacterium aurum. Structure and biogenetic implications. Eur. J. Biochem. 177:631–635
    [Google Scholar]
  20. Larsson L., Jantzen E., Johnsson J. 1985; Gas chromatographic fatty acid profiles for characterization of mycobacteria: an interlaboratory methodological evaluation. Eur. J. Clin. Microbiol. 4:483–487
    [Google Scholar]
  21. Lévy-Frébault V., Goh K. S., David H. L. 1986; Mycolic acid analysis for clinical identification of Mycobacterium avium and related mycobacteria. J. Clin. Microbiol. 24:835–839
    [Google Scholar]
  22. Lévy-Frébault V., Grimont F., Grimont P. A. D., David H. L. 1986; Deoxyribonucleic acid relatedness study of the Mycobacterium fortuitum-Mycobacterium chelonae complex. Int. J. Syst. Bacteriol. 36:458–460
    [Google Scholar]
  23. Lévy-Frébault V., Pangon B., Buré A., Katlama C., Marche C., David H. L. 1987; Mycobacterium simiae and Mycobacterium avium-intracellulare mixed infection in acquired immune deficiency syndrome. J. Clin. Microbiol. 25:154–157
    [Google Scholar]
  24. Lévy-Frébault V., Rafidinarivo J., Promé J. C., Grandry J., Boisvert H., David H. L. 1983; Mycobacterium fallax sp. nov. Int. J. Syst. Bacteriol. 33:333–346
    [Google Scholar]
  25. Luquin M., Ausina V., López-Calahorra F., Belda F., García-Barceló M., Celma C., Prats G. 1991; Evaluation of practical chromatographic procedures for identification of clinical isolates of mycobacteria. J. Clin. Microbiol. 29:120–130
    [Google Scholar]
  26. Luquin M., Lanéelle M. A., Ausina V., García-Barceló M., Belda F., Alonso C., Prats G. 1991; Distribution of a novel mycolic acid in species of the genus Mycobacterium. Int. J. Syst. Bacteriol. 41:390–394
    [Google Scholar]
  27. Luquin M., Margarit L., Comdom M. J., Ausina V. 1987; Mycolic acids of Mycobacterium porcinum. Int. J. Syst. Bacteriol. 37:75–77
    [Google Scholar]
  28. Luquin M., Roussel J., López-Calahorra F., Lanéelle G., Ausina V., Lanéelle M. A. 1990; A novel mycolic acid in a Mycobacterium sp. from the environment. Eur. J. Biochem. 192:753–759
    [Google Scholar]
  29. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory; Cold Spring Harbor, N.Y:
    [Google Scholar]
  30. Minnikin D. E., Hutchinson I. A., Caldicott A. B., Goodfellow M. 1980; Thin-layer chromatography of methanolysates of mycolic acid containing bacteria. J. Chromatogr. 188:221–233
    [Google Scholar]
  31. Minnikin D. E., Minnikin S. M., Goodfellow M. 1982; The oxygenated mycolic acids of Mycobacterium fortuitum, Mycobacterium farcinogenes and Mycobacterium senegalense. Biochim. Biophys. Acta 712:616–620
    [Google Scholar]
  32. Minnikin D. E., Minnikin S. M., Parlett J. H., Goodfellow M. 1985; Mycolic acids patterns of some rapidly-growing species of Mycobacterium. Zentralbl. Bakteriol. Parasitenkd. Infektionskr. Hyg. Abt. 1 Orig. Reihe A 259:446–460
    [Google Scholar]
  33. Minnikin D. E., Minnikin S. M., Parlett J. H., Goodfellow M., Magnusson M. 1984; Mycolic acid patterns of some species of Mycobacterium. Arch. Microbiol. 139:225–231
    [Google Scholar]
  34. Minnikin D. E., Parlett J. H., Magnusson M., Ridell M., Lind A. 1984; Mycolic acid patterns of representatives of Mycobacterium bovis BCG. J. Gen. Microbiol. 130:2733–2736
    [Google Scholar]
  35. Minnikin D. E., Polgar N. 1967; The methoxymycolic and ketomycolic acids from human tubercle bacilli. Chern. Commun. (J. Chern. Soc. Sect. D) 1967:1172–1174
    [Google Scholar]
  36. Moore W. E. C., Moore L. V. H. 1989 Index of the bacterial and yeast nomenclature changes. 41 American Society for Microbiology; Washington, D.C:
    [Google Scholar]
  37. Owen R. J., Pitcher D. 1985 Current methods for estimating DNA base composition and levels of DNA-DNA hybridization. 67–93 Goodfellow M., Minnikin D. E.ed Chemical methods in bacterial systematics Academic Press, Inc. (London), Ltd.; London:
    [Google Scholar]
  38. Portaels F., Asselineau C., Baess I., Daffé M., Dobson G., Draper P., Gregory D., Hall R. M., Imaeda T., Jenkins P. A., Lanéelle M. A., Larsson L., Magnusson M., Minnikin D. E., Pattyn S. R., Wieten G., Wheeler P. R. 1986; A cooperative taxonomic study of mycobacteria isolated from armadillo infected with Mycobacterium leprae. J. Gen. Microbiol. 132:2693–2707
    [Google Scholar]
  39. Silcox V. A., Good R. C., Floyd M. M. 1981; Identification of clinically significant Mycobacterium fortuitum complex isolates. J. Clin. Microbiol. 14:686–691
    [Google Scholar]
  40. Skerman V. B. D., McGowan V., Sneath P. H. A.ed 1980; Approved lists of bacterial names. Int. J. Syst. Bacteriol. 30:225–420
    [Google Scholar]
  41. Sokal R. R., Sneath P. H. A. 1963 Principles of numerical taxonomy. W. H. Freeman and Co.; San Francisco:
    [Google Scholar]
  42. Szabo I., Vandra E. 1963; Mycobacterium minetti (Penso et al., 1952): bacteriological and epidemiological observations. Acta Microbiol. Acad. Sci. Hung. 10:215–223
    [Google Scholar]
  43. Tomiyasu J., Yano I. 1984; Separation and analysis of novel polyunsaturated mycolic acids from a psicrophilic, acid fast bacterium, Gordona aurantiaca. Eur. J. Biochem. 139:173–180
    [Google Scholar]
  44. Tsukamura M. 1967; Identification of mycobacteria. Tubercle 48:311–338
    [Google Scholar]
  45. Valero-Guillen P. L., Pacheco F., Martin-Luengo F. 1985; Fatty acid composition and mycolic acid pattern of some chromogenic mycobacteria. J. Appl. Bacteriol. 59:113–126
    [Google Scholar]
  46. Vestal A. 1975 Procedures for the isolation and identification of mycobacteria. Publication (CDC) 76-8230. Centers for Disease Control; Atlanta:
    [Google Scholar]
  47. Wayne L. G., Engbaek H. C., Engel H. W. B., Froman S., Gross W., Hawkins J., Käppler W., Karlson A. G., Kleeberg H. H., Krasnow I., Kubica G. P., McDurmont C., Nel E. E., Pattyn S. R., Schröder K. H., Showalter S., Tamok I., Tsukamura M., Vergmann B., Wolinsky E. 1974; Highly reproducible techniques for use in systematic bacteriology in the genus Mycobacterium•, tests for pigment, urease, resistance to sodium chloride, hydrolysis of Tween 80, and β-galactosidase. Int. J. Syst. Bacteriol. 24:412–419
    [Google Scholar]
  48. Wayne L. G., Engel H. W. B., Grassi C., Gross W., Hawkins J., Jenkins P. A., Käppler W., Kleeberg H. H., Krasnow I., Nel E. E., Pattyn S. R., Richards P. A., Showalter S., Slosarek M., Szabo I., Tarnok I., Tsukamura M., Vergmann B., Wolinsky E. 1976; Highly reproducible techniques for use in systematic bacteriology in the genus Mycobacterium•, tests for niacin and catalase and for resistance to isoniazid, thiophene-2-carboxylic acid hydrazide, hydroxylamine, and p-nitrobenzoate. Int. J. Syst. Bacteriol. 26:311–318
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-42-4-529
Loading
/content/journal/ijsem/10.1099/00207713-42-4-529
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error