1887

Abstract

Acholeplasma strains were isolated from the nasopharynx of a horse (strain PN525 [T = type strain]) and the feces of a rabbit (strain B1). One clone of strain PN525 and one clone of strain B1 were examined in detail. These clones were indistinguishable from each other and were serologically distinct from the previously described and spp. The strains had the following properties: Guanine-plus-cytosine content of 31 mol%; sterol was not required for growth, which occurred under both aerobic and anaerobic conditions; glucose was metabolized; and arginine was hydrolyzed. Strain PN525 (= NCTC 11723) is the type strain of a new species,

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-42-4-513
1992-10-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/42/4/ijs-42-4-513.html?itemId=/content/journal/ijsem/10.1099/00207713-42-4-513&mimeType=html&fmt=ahah

References

  1. Aluotto B. B., Wittier R. G., Williams C. O., Faber J. E. 1970; Standardized bacteriologic techniques for the characterization of Mycoplasma species. Int. J. Syst. Bacteriol. 20:35–58
    [Google Scholar]
  2. Angulo A. F., Doeksen M., Hill A. C., Polak-Vogelzang A. A. 1987; Isolation of Acholeplasmatales from rabbit faeces. Lab. Anim. 21:201–204
    [Google Scholar]
  3. Barber T. L., Fabricant J. 1971; Identification of Mycoplasmatales: characterization procedures. Appl. Microbiol. 21:600–605
    [Google Scholar]
  4. Boatman E. S. 1979 Morphology and ultrastructure of Mycoplasmatales,. 63–102 Barile M. F., Razin S.ed The mycoplasmas 1 Academic Press, Inc.; New York:
    [Google Scholar]
  5. Clyde W. A. Jr. 1964; Mycoplasma species identification based upon growth inhibition by specific antisera. J. Immunol. 92:958–965
    [Google Scholar]
  6. Edward D. G. ff 1971; Determination of sterol requirement for Mycoplasmatales. J. Gen. Microbiol. 69:205–210
    [Google Scholar]
  7. Edward D. G. ff., Fitzgerald W. A. 1951; Cholesterol in the growth of organisms of the pleuropneumonia group. J. Gen. Microbiol. 5:576–586
    [Google Scholar]
  8. Fabricant J., Freundt E. A. 1967; Importance of extension and standardization of laboratory tests for the identification and classification of mycoplasma. Ann. N.Y. Acad. Sci. 143:50–58
    [Google Scholar]
  9. Fallon R. J., Whittlestone P. 1969; Isolation, cultivation and maintenance of mycoplasmas. Methods Microbiol 3B:211–267
    [Google Scholar]
  10. Freundt E. A., Andrews B. E., Ernø H., Kunze M., Black F. T. 1973; The sensitivity of Mycoplasmatales to sodiumpolyanethol sulfonate and digitonin. Zentralbl. Bakteriol. Parasitenkd. Infektionskr. Hyg. Abt. 1 Grig. Reihe A 225:104–112
    [Google Scholar]
  11. Gross-Bellard M. J., Oudet P., Chambon P. 1973; Isolation of high molecular weight DNA from mammalian cells. Eur. J. Biochem. 36:32–38
    [Google Scholar]
  12. Herderscheê D. 1963; An improved medium for isolation of the Eaton agent. Antonie van Leeuwenhoek 29:154–156
    [Google Scholar]
  13. Hill A. C. 1971; Mycoplasma caviae, a new species. J. Gen. Microbiol. 65:109–113
    [Google Scholar]
  14. Hill A. C. 1977; The metabolic inhibition test for mycoplasmas based on phosphatase production. J. Hyg. 79:391–393
    [Google Scholar]
  15. Hill A. 1978; Demonstration of mycoplasmas in tissue by the immunoperoxidase technique. J. Infect. Dis. 137:152–154
    [Google Scholar]
  16. Klieneberger-Nobel E. 1962 Morphology of pleuropneumonialike organisms. 23–56 Klieneberger-Nobel E.ed Pleuropneumonia-like organisms (PPLO) Mycoplasmataceae Academic Press, Inc.; New York:
    [Google Scholar]
  17. Leach R. H. 1976; The inhibitory effect of arginine on growth of some mycoplasmas. J. Appl. Bacteriol. 41:259–264
    [Google Scholar]
  18. Manchee R. J., Taylor-Robinson D. 1968; Haemadsorption and haemagglutination by mycoplasmas. J. Gen. Microbiol. 50:465–478
    [Google Scholar]
  19. Morton H. E., Roberts R. J. 1967; Production of antimycoplasma (PPLO) antibodies in rabbits. Proc. Soc. Exp. Biol. Med. 125:538–543
    [Google Scholar]
  20. Mouches C., Bové J. M. 1983; Electrophoretic characterization of mycoplasma proteins. Methods Mycoplasmol. 1:241–255
    [Google Scholar]
  21. Polak-Vogelzang A. A., Hagenaars R., Nagel J. 1978; Evaluation of an indirect immunoperoxidase test for identification of Acholeplasma and Mycoplasma. J. Gen. Microbiol. 106:241–249
    [Google Scholar]
  22. Polak-Vogelzang A. A., Reijgers R., Hekkens F. E. N. 1980; Isolation of Mycoplasma hyorhinis and Mycoplasma fermentans from cell cultures. J. Biol. Stand. 8:243–254
    [Google Scholar]
  23. Purcell R. H., Taylor-Robinson D., Wong D. C., Chanock R. M. 1966; A color test for the measurement of antibody to the non-acid-forming human mycoplasma species. Am. J. Epidemiol. 84:51–66
    [Google Scholar]
  24. Purcell R. H., Taylor-Robinson D., Wong D. C., Chanock R. M. 1966; Color test for the measurement of antibody to T-strain mycoplasmas. J. Bacteriol. 92:6–12
    [Google Scholar]
  25. Razin S. 1983; Pigmented carotenoids. Methods Mycoplasmol. 1:375–376
    [Google Scholar]
  26. Razin S., Tully J. G. 1970; Cholesterol requirement of mycoplasmas. J. Bacteriol. 102:306–310
    [Google Scholar]
  27. Schildkraut C. L., Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its buoyant density in CsCl. J. Mol. Biol. 4:430–443
    [Google Scholar]
  28. Shepard M. C., Howard D. R. 1970; Identification of “T” mycoplasmas in primary agar cultures by means of a direct test for urease. Ann. N.Y. Acad. Sci. 174:809–819
    [Google Scholar]
  29. Taylor-Robinson D., Purcell R. H., Wong D. C., Chanock R. M. 1966; A colour test for the measurement of antibody to certain mycoplasma species based upon the inhibition of acid production. J. Hyg. 64:91–104
    [Google Scholar]
  30. Taylor-Robinson D., Williams M. H., Haig D. A. 1968; The isolation and comparative biological and physical characteristics of T-mycoplasmas of cattle. J. Gen. Microbiol. 54:33–46
    [Google Scholar]
  31. Timms L. 1967; Isolation and identification of avian mycoplasmas. J. Med. Lab. Technol. 24:79–89
    [Google Scholar]
  32. Tully J. G. 1965; Biochemical, morphological and serological characterization of mycoplasma of murine origin. J. Infect. Dis. 115:171–185
    [Google Scholar]
  33. Tully J. G. 1983; Tests for digitonin sensitivity and sterol requirements. Methods Mycoplasmol. 1:355–357
    [Google Scholar]
  34. Tully J. G., Razin S. 1969; Characteristics of a new sterol non-requiring mycoplasma. J. Bacteriol. 98:970–978
    [Google Scholar]
  35. Tully J. G., Whitcomb R. F., Clark H. F., Williamson D. L. 1977; Pathogenic mycoplasmas: cultivation and vertebrate pathogenicity of a new spiroplasma. Science 195:892–894
    [Google Scholar]
  36. Venable J. H., Coggeshall R. 1965; A simplified lead citrate stain for use in electron microscopy. J. Cell Biol. 25:407–408
    [Google Scholar]
  37. Williams C. O., Wittier R. G. 1971; Hydrolysis of esculin and phosphatase production by members of the order Mycoplasmatales which do not require sterol. Int. J. Syst. Bacteriol. 21:73–77
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-42-4-513
Loading
/content/journal/ijsem/10.1099/00207713-42-4-513
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error