Acholeplasma strains were isolated from the nasopharynx of a horse (strain PN525 [T = type strain]) and the feces of a rabbit (strain B1). One clone of strain PN525 and one clone of strain B1 were examined in detail. These clones were indistinguishable from each other and were serologically distinct from the previously described and spp. The strains had the following properties: Guanine-plus-cytosine content of 31 mol%; sterol was not required for growth, which occurred under both aerobic and anaerobic conditions; glucose was metabolized; and arginine was hydrolyzed. Strain PN525 (= NCTC 11723) is the type strain of a new species,


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