A psychrotrophic sp., sp. nov., was isolated from the adherent bacterial epiflora of Atlantic halibut ( L.) eggs and was shown to be an opportunistic pathogen for halibut eggs and larvae. The strains which we isolated had the enzymatic capacity to dissolve both the chorion and the zona radiata of the egg shells. A total of 35 isolates were characterized by using morphological and biochemical tests. These strains were rod shaped, gram negative, Kovacs oxidase positive, and pale yellow and exhibited gliding motility. They did not produce acid from any of the wide range of carbohydrates tested. Our isolates had the ability to degrade gelatin, tyrosine, DNA, and Tween 80. Starch, cellulose, and chitin were not degraded. The strains were catalase and nitrate reductase positive, did not produce HS, and did not grow under anaerobic conditions. resembles , but differs from the latter species in several biochemical and physiological characteristics. DNAs from strains had guanine-plus-cytosine contents which ranged from 30.3 to 32.0 mol% (strains EKC001, EKD002 [T = type strain], and VKB004), and DNA-DNA hybridization studies revealed levels of relatedness between EKD002 and NCMB 2154 and NCMB 2153 of 42.7 and 30.0%, respectively. Compared with previously described and spp. with low guanine-plus-cytosine contents, constitutes a new species. Strain EKD002 (= NCIMB 13127) is the type strain of the new species.


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