A new species, Brachybacterium nesterenkovii, is proposed for a group of coryneform bacteria that were isolated from milk products. These organisms have morphological, biochemical, and chemotaxonomic characteristics that are peculiar to the genus Brachybacterium. In contrast to strains of the only previously described species of the genus, Brachybacterium faecium, the representatives of the new species lack glycine in their peptidoglycan, although the peptidoglycan is of the same general type, and have large amounts of rhamnose in their cells. The strains of B. nesterenkovii exhibit no serological relationship with strains of B. faecium; in a numerical phenotypic analysis the two species were easily separated and formed clear-cut clusters. DNA-DNA hybridization between the type strains of B. faecium and B. nesterenkovii showed a level of homology of 22%. Strain 35 (= IMV Ac-752) is the type strain of B. nesterenkovii.
AndreevL. V., GalchenkoV. R.1978; Fatty acid composition and identification of methanotrophic bacteria. Dokl. Akad. Nauk SSSR 239:1465–1468 In Russian.
Bab’evaI. P.1966 Study of soil microorganisms in pure cultures. 68–69Methods for studying soil microorganisms and their metabolites Moscow State University; Moscow: In Russian.
Baird-ParkerA. C.1979 Methods for identifying staphylococci and micrococci. 201–210SkinnerF. A., LovelockD. W.edIdentification methods for microbiologists Academic Press; London:
BogdanovV. M., KorolyovaN. S., BannikovaL. A.1967 Microbiological control on the enterprises of dairy industries. Pishchevaya Promstyshlennost; Moscow: In Russian.
BogdanovskyD., Interschick-NieblerE., SchleiferK. H., FiedlerF., KandlerO.1971; y-Glutamyl-glutamic acid, an interpeptide bridge in murein of some micrococci and Arthrobacter sp. Eur. J. Biochem. 22:173–178
CollinsM. D., BrownJ., JonesD.1988; Brachybacterium faecium gen. nov., sp. nov., a coryneform bacterium from poultry deep litter. Int. J. Syst. Bacteriol. 38:45–48
GordonR. E., SmithM. M.1953; Rapidly growing acid-fast bacteria. I. Species descriptions of Mycobacterium phlei Lehmann and Neumann and Mycobacterium smegmatis (Trevisan) Lechmann and Neumann. J. Bacteriol. 66:41–48
JankeD., Al-MofarjiT., StraubeG., SchumannP., PrauserH.1988; Critical steps in degradation of chloroaromatics by rhodococci. I. Initial enzyme reactions involved in catabolism of aniline, phenol and benzoate by Rhodococcus sp. An 117 and An 213. J. Basic Microbiol. 28:509–518
KvasnikovE. I., GvozdyakO. R., SchkarubaV. V.1987; Distribution of gram-positive aerobic cocci in the environment. Mikrobiol. Zh. (Kiev) 49:48–51 In Russian.
MarmurJ., DotyP.1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J. Mol. Biol. 5:109–118
MeyerS. A., SchleiferK. H.1978; Deoxyribonucleic acid reassociation in the classification of coagulase-positive staphylococci. Arch. Microbiol. 117:183–188
MinnikinD. E., AlshamaonyL., GoodfellowM.1975; Determination of Mycobacterium, Nocardia and related taxa by thin-layer chromatographic analysis of whole-organism methanolysates. J. Gen. Microbiol. 88:200–204
NesterenkoO. A., KvasnikovE. I., VasyurenkoZ. P., GvozdyakO. R., StognyI. P, NoginaT. M., ShkarubaV. V., KishchenkoV. A.1986; Biological characteristics of coryneform bacteria with A 4-y peptidoglycan variation. Deposited in VINITI 30.10.86. N 7488-B86. (In Russian).
NoginaT. M., GvozdyakO. R., SadovnikovYu. S., ShkarubaV. V., KuberskayaS. L.1991; Phenotypical study and numerical analysis of the strains of coryneform bacteria with A 4y peptidoglycan variation. Mikrobiol. Zh. (Kiev) 53:3–10 In Russian.
NoginaT. M., StognyI. P., GvozdyakO. R., NesterenkoO. A., KvasnikovE. I.1987; Rapid method of diaminopimelic acid determination in bacterial cells. Mikrobiol. Zh. (Kiev) 49:98–100 In Russian.
NoginaT. M., VarbanetsL. D., NesterenkoO. A., StognyI. P., GvozdyakO. R., KishchenkoV. A.1989; Amino acid composition of peptidoglycans obtained by different methods from coryneform bacteria cells. Mikrobiol. Zh. (Kiev) 51:16–19 In Russian.
SchleiferK. H.1981; Die Klassifikation von Staphylococcus und Micrococcus. Ein Beispiel fur die moderne Bakteriensystematik. Forum Mikrobiol. 4:272–274
SierraG.1957; A simple method for the detection of lipolytic activity of microorganisms and some observations on the influence of the contact between cells and fatty substrates. Antonie van Leeuwenhoek J. Microbiol. SeroL 23:15–22