Nineteen gram-negative, aerobic, biodegradative isolates were identified by using a polyphasic taxonomic approach. The presence of the specific polyamine 2-hydroxyputrescine and the presence of a ubiquinone with eight isoprenoid units in the side chain (ubiquinone Q-8) allowed allocation of these organisms to the beta subclass of the . On the basis of the results of additional characterization experiments (i.e., API 20NE tests, determinations of soluble protein patterns, and DNA-DNA hybridization experiments), we classified six isolates as either , or subsp. . By using the same criteria we allocated two additional isolates to the genus . A comparison of a 16S rRNA fragment (positions 1220 to 1377; nomenclature) indicated that the remaining isolates should be allocated as follows: One is a member of and one is a member of , as confirmed by the results of additional DNA-DNA hybridizations; two others probably belong to the family ; six are related to “”; and one, strain NRRL 12228, occupies an isolated position.


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