1887

Abstract

Two new species of aerobic, gram-negative, peritrichously flagellated or nonmotile marine bacteria usually forming star-shaped aggregates were isolated from northeastern Atlantic Ocean bottom sediments. These organisms resembled eight star-shaped-aggregate-forming bacterial species from the Baltic Sea originally ascribed to the genus but not included on the because of their questionable relationships to true agrobacteria. These two sets of star-shaped-aggregate-forming bacteria were compared by means of phenotypic data, DNA base compositions, DNA-DNA relatedness, and one-dimensional electrophoretic analysis of low-molecular-weight RNAs (5S rRNA and tRNA). According to the results of genotyping, the northeastern Atlantic Ocean isolates and three of the Baltic Sea species formed a group of closely related bacteria that could not be excluded from the genus with certainty. Until more genotypic data are available, these five marine species are regarded as a distinct subdivision of the genus consisting of sp. nov. (type strain, 1480 = DSM 5823), sp. nov. (type strain, 1513 = DSM 5824), sp. nov. nom. rev. emend. (type strain, ATCC 25652), sp. nov. nom. rev. emend. (type strain, ATCC 25655), and sp. nov. nom. rev. emend, (type strain, ATCC 15215). “” proved to be a later subjective synonym of , which had priority. The remaining four Baltic Sea species, “,” and “,” could not be placed in the new subdivision of .

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-42-1-133
1992-01-01
2024-12-06
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/42/1/ijs-42-1-133.html?itemId=/content/journal/ijsem/10.1099/00207713-42-1-133&mimeType=html&fmt=ahah

References

  1. Ahrens R. 1968; Taxonomische Untersuchungen an sternbildenden Agrobacterium-Arten aus der westlichen Ostsee. Kieler Meeresforsch. 24:147–173
    [Google Scholar]
  2. Ahrens R., Rheinheimer G. 1967; Über einige sternbildende Bakterien aus der Ostsee. Kieler Meeresforsch. 23:127–136
    [Google Scholar]
  3. Allen O. N., Holding A. J. 1974 Genus II. Agrobacterium Conn 1942, 359. Nom. gen. cons. Opin. 33, Jud. Comm. 1970, 10. 264–267 Buchanan R. E., Gibbons N. E.ed Bergey’s manual of determinative bacteriology, 8th. The Williams & Wilkins Co.; Baltimore:
    [Google Scholar]
  4. Baumann L., Bowditch R. D., Baumann P. 1983; Description of Deleya gen. nov. created to accommodate the marine species Alcaligenes aestus, A. pacificus,A. cupidus,A. venustus, and Pseudomonas marina. Int. J. Syst. Bacteriol. 33:793–802
    [Google Scholar]
  5. De Ley J. 1968; Molecular biology and bacterial phylogeny. EVol. Biol. 2:103–156
    [Google Scholar]
  6. De Ley J. 1970; Reexamination of the association between melting point, buoyant density, and chemical base composition of deoxyribonucleic acid. J. Bacteriol. 101:738–754
    [Google Scholar]
  7. De Ley J. 1978; Modern molecular methods in bacterial taxonomy: evaluation, application, prospects. 347–357Proceedings of the 4th International Conference on Plant Pathology and BacteriologyAngers1978
    [Google Scholar]
  8. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur. J. Biochem. 12:133–142
    [Google Scholar]
  9. De Smedt J., De Ley J. 1977; Intra- and intergeneric similarities of Agrobacterium ribosomal ribonucleic acid cistrons. Int. J. Syst. Bacteriol. 27:222–240
    [Google Scholar]
  10. Gillis M., De Ley J., De Cleene M. 1970; The determination of molecular weight of bacterial genome DNA from renaturation rates. Eur. J. Biochem. 12:143–153
    [Google Scholar]
  11. Grierson D. 1982 Gel electrophoresis of RNA. 1–38 Rickwood D., Hames B. D.ed Gel electrophoresis of nucleic acids IRL Press Ltd.; Oxford:
    [Google Scholar]
  12. Grimont P. A. D. 1988; Use of DNA reassociation in bacterial classification. Can. J. Microbiol. 34:541–546
    [Google Scholar]
  13. Hamana K., Matsuzaki S. 1990; Polyamines and their biosynthetic activities in nonphytopathogenic marine agrobacteria. Can. J. Microbiol. 36:567–572
    [Google Scholar]
  14. Hartford T., Sneath P. H. A. 1988; Distortion of taxonomic structure from DNA relationships due to different choice of reference strains. Syst. Appl. Microbiol. 10:241–250
    [Google Scholar]
  15. Hartford T., Sneath P. H. A. 1990; Experimental error in DNA-DNA pairing: a survey of the literature. J. Appl. Bacteriol. 68:527–542
    [Google Scholar]
  16. Hoile M. G. 1988; Identification of bacteria by low molecular weight RNA profiles: a new chemotaxonomic approach. J. Microbiol. Methods 8:235–248
    [Google Scholar]
  17. Hofle M. G. 1990; Transfer RNAs as genotypic fingerprints of eubacteria. Arch. Microbiol. 153:299–304
    [Google Scholar]
  18. Hofle M. G. 1990 RNA chemotaxonomy of bacterial isolates and natural microbial communities. 129–158 Overbeck J., Chrost R.ed Aquatic microbial ecology—biochemical and molecular approaches Springer-Verlag; New York:
    [Google Scholar]
  19. Huss V. A. R., Festl H., Schleifer K. H. 1983; Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst. Appl. Microbiol. 4:184–192
    [Google Scholar]
  20. Jenni B., Aragno M., Wiegel J. K. W. 1987; Numerical analysis and DNA-DNA hybridization studies on Xanthobacter and emendation of Xanthobacter flavus. Syst. Appl. Microbiol. 9:247–253
    [Google Scholar]
  21. Jennings A. V. 1898-1900; On a new genus of bacteria (Astrobacter). Proc. R. Irish Acad. Dublin Ser. 3 5:312–316 and plates VI and VIL
    [Google Scholar]
  22. Jordan D. C. 1984 Family III. Rhizobiaceae Conn 1938, 321. 234–235 Krieg N. R., Holt J. G.ed Bergey’s manual of systematic bacteriology 1 The Williams & Wilkins Co.; Baltimore:
    [Google Scholar]
  23. Kersters K., De Ley J. 1984 Genus III. Agrobacterium Conn 1942, 359. 244–254 Krieg N. R., Holt J. G.ed Bergey’s manual of systematic bacteriology 1 The Williams & Wilkins Co.; Baltimore:
    [Google Scholar]
  24. Kersters K., De Ley J. 1984 Genus Alcaligenes Castellani and Chalmers 1919, 936. 361–373 Krieg N. R., Holt J. G.ed Bergey’s manual of systematic bacteriology 1 The Williams & Wilkins Co.; Baltimore:
    [Google Scholar]
  25. Knosel D. 1984 Genus IV. Phyllobacterium (ex Knbsel 1962) nom. rev. (Phyllobacterium Knosel 1962, 96). 254–256 Krieg N. R., Holt J. G.ed Bergey’s manual of systematic bacteriology 1 The Williams & Wilkins Co.; Baltimore:
    [Google Scholar]
  26. Kolodny G. M. 1984; An improved method for increasing the resolution and sensitivity of silver staining of nucleic acid bands in polyacrylamide gels. Anal. Biochem. 138:66–67
    [Google Scholar]
  27. Koops H.-P., Harms H. 1985; Deoxyribonucleic acid homologies among 96 strains of ammonia-oxidizing bacteria. Arch. Microbiol. 141:214–218
    [Google Scholar]
  28. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J. Mol. Biol. 3:208–218
    [Google Scholar]
  29. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J. Mol. Biol. 5:109–118
    [Google Scholar]
  30. Palleroni N. J. 1984 Genus Pseudomonas Migula 1894, 237. 141–199 Krieg N. R., Holt J. G.ed Bergey’s manual of systematic bacteriology 1 The Williams & Wilkins Co.; Baltimore:
    [Google Scholar]
  31. Rüger H.-J. 1985; Benthic studies of the northwest African upwelling region: structures and catabolic potentials of mesophilic bacterial communities. Veröff. Inst. Meeresforsch. Bremerhaven 21:97–114
    [Google Scholar]
  32. Rüger H.-J. 1988; Substrate-dependent cold adaptations in some deep-sea sediment bacteria. Syst. Appl. Microbiol. 11:90–93
    [Google Scholar]
  33. Rüger H.-J., Richter G. 1979; Bacillus globisporus subsp. marinus subsp. nov. Int. J. Syst. Bacteriol. 29:196–203
    [Google Scholar]
  34. Rüger H.-J., Tan T. L. 1983; Separation of Alcaligenes denitrificans sp. nov., nom. rev. from Alcaligenes faecalis on the basis of DNA base composition, DNA homology, and nitrate reduction. Int. J. Syst. Bacteriol. 33:85–89
    [Google Scholar]
  35. Rüger H.-J., Tan T. L., Hentzschel G., Naguib M. 1983; New denitrifying Agrobacterium and Alcaligenes strains from Weser estuary sediments: taxonomy and physiology. Veröff. Inst. Meeresforsch. Bremerhaven 19:229–243
    [Google Scholar]
  36. Schleifer K. H., Stackebrandt E. 1983; Molecular systematics of prokaryotes. Annu. Rev. Microbiol. 37:143–187
    [Google Scholar]
  37. Siebert G., Schwartz W. 1956; Untersuchungen über das Vorkommen von Mikroorganismen in entstehenden Sedimenten. Arch. Hydrobiol. 52:321–366
    [Google Scholar]
  38. Skerman V. B. D., McGowan V., Sneath P. H. A.ed 1980; Approved lists of bacterial names. Int. J. Syst. Bacteriol. 30:225–420
    [Google Scholar]
  39. Slade P. J., Collins-Thompson D. L. 1991; Differentiation of the genus Listeria from other gram-positive species based on low molecular weight (LMW) RNA profiles. J. Appl. Bacteriol. 70:355–360
    [Google Scholar]
  40. Stackebrandt E., Murray R. G. E., Trüper H. G. 1988; Proteobacteria classis nov., a name for the phylogenetic taxon that includes the “purple bacteria and their relatives.”. Int. J. Syst. Bacteriol. 38:321–325
    [Google Scholar]
  41. Stapp C., Knosel D. 1954; Zur Genetik stembildender Bakterien. Zentralbl. Bakteriol. Parasitenkd. Infektionskr. Hyg. Abt. 2 108:243–259
    [Google Scholar]
  42. Weyland H., Rüger H.-J., Schwarz H. 1970; Zur Isolierung und Identifizierung mariner Bakterien: ein Beitrag zur Standardisierung und Entwicklung adäquater Methoden. Veröff. Inst. Meeresforsch. Bremerhaven 12:269–296
    [Google Scholar]
/content/journal/ijsem/10.1099/00207713-42-1-133
Loading
/content/journal/ijsem/10.1099/00207713-42-1-133
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error