1887

Abstract

Antigenic and genomic relatedness among , and was analyzed by enzyme-linked immunosorbent assay, Western blotting (immunoblotting) and DNA-DNA hybridization. and were serologically related, and their Western blot antigen profiles were nearly identical. Two antigens of corresponding to the 28- and 51-kDa antigens of were apparently larger than the antigens, and the 55-kDa antigen of appeared to be unique to this species. The 110-, 70-, and 44-kDa antigens of these two species were identical, as determined by the use of monospecific antibodies. DNA homology between these two species was high. On the other hand, was antigenically least reactive with the antisera to and . However, a dog convalescent-stage antiserum recognized antigens in the other two species which were different from those recognized by their homologous antisera. Similarly, homology between the DNA of and the DNAs of the other two species was very minimal. These results indicate that and are closely related both at the genomic and antigenic levels and that the relationship of these two species with is minimal.

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1992-01-01
2023-02-06
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References

  1. Brenner D. J. 1983; Impact of modern taxonomy on clinical microbiology. ASM News 49:58–63
    [Google Scholar]
  2. Dawson J., Anderson B., Fishbein D., Sanchez J., Goldsmith C., Wilson K., Duntley C. 1991 9th Sesquiannu. Meet. Am. Soc. Rickettsiol. Rickettsial Dis. abstr. 11 20
    [Google Scholar]
  3. Dutta S. K., Mattingly B. L., Shankarappa B. 1989; Antibody response to Ehrlichia risticii and antibody reactivity to component antigens in horses with induced Potomac horse fever. Infect. Immun. 57:2959–2962
    [Google Scholar]
  4. Dutta S. K., Myrup A. C., Rice R. M., Robi M. G., Hammond R. C. 1985; Experimental reproduction of Potomac horse fever in horses with newly isolated ehrlichia organism. J. Clin. Microbiol. 22:265–269
    [Google Scholar]
  5. Dutta S. K., Rice R. M., Hughes T. D., Savage P. K., Myrup A. C. 1987; Detection of serum antibodies against Ehrlichia risticii in Potomac horse fever by enzyme-linked immunosorbent assay. Vet. Immunol. Immunopathol. 14:85–92
    [Google Scholar]
  6. Dutta S. K., Shankarappa B., Mattingly-Napier B. L. 1991; Molecular cloning and analysis of recombinant major antigens of Ehrlichia risticii. Infect. Immun. 59:1162–1169
    [Google Scholar]
  7. Dutta S. K., Shankarappa B., Thaker S. R., Mattingly-Napier B. L. 1990; DNA restriction endonuclease cleavage pattern and protein antigen profile of Ehrlichia risticii. Vet. Microbiol. 25:29–38
    [Google Scholar]
  8. Harkess J. R., Ewing S. A., Crutcher J. M., Kudlac J., McKee G., Istre G. R. 1989; Human ehrlichiosis in Oklahoma. J. Infect. Dis. 159:576–579
    [Google Scholar]
  9. Holland C. J. 1988Annu. Symp. Potomac Horse FeverLaurel, Md.24 May 1988
    [Google Scholar]
  10. Holland C. J., Ristic M., Cole A. I., Johnson P., Baker G., Goetz T. 1985; Isolation, experimental transmission and characterization of the causative agent of Potomac horse fever. Science 277:522–526
    [Google Scholar]
  11. Holland C. J., Weiss E., Burgdorfer W., Cole A. I., Kakoma I. 1985; Ehrlichia risticii sp. nov.: etiological agent of equine monocytic ehrlichiosis (synonym, Potomac horse fever). Int. J. Syst. Bacteriol. 35:524–526
    [Google Scholar]
  12. Lauer B. A., Reller L. B., Mirrett S. 1981; Comparison of acridine orange and gram stains for detection of microorganisms in cerebrospinal fluid and other clinical specimens. J. Clin. Microbiol. 14:201–215
    [Google Scholar]
  13. Maeda K., Markowitz N., Hawley R. C., Ristic M., Cox D., McDade J. E. 1987; Human infection with Ehrlichia canis, a leukocytic rickettsia. N. Engl. J. Med. 316:853–856
    [Google Scholar]
  14. Nyindo M. B. A., Ristic M., Huxsoll D. L., Smith A. R. 1971; Tropical canine pancytopenia—in vitro cultivation of the causative agent, Ehrlichia canis. Am. J. Vet. Res. 32:1651–1658
    [Google Scholar]
  15. Oaks E. V., Rice R. M., Kelly D. J., Stover C. K. 1989; Antigenic and genetic relatedness of eight Rickettsia tsutsugamushi antigens. Infect. Immun. 57:3116–3122
    [Google Scholar]
  16. Peterson L. R., Sawyer L. A., Fishbein D. B., Kelley P. W., Thomas R. J., Magnarelli L. A., Redus M., Dawson J. E. 1989; An outbreak of ehrlichiosis in members of an army reserve unit exposed to ticks. J. Infect. Dis. 159:562–568
    [Google Scholar]
  17. Rikihisa Y. 1991; The tribe Ehrlichieae and ehrlichial diseases. Clin. Microbiol. Rev. 4:286–308
    [Google Scholar]
  18. Rikihisa Y., Perry B. D. 1985; Causative ehrlichial organisms in Potomac horse fever. Infect. Immun. 49:513–517
    [Google Scholar]
  19. Ristic M. 1986 Pertinent characteristics of leukocytic rickettsiae of humans and animals. 182–187 Leive L.ed Microbiology—1986 American Society for Microbiology; Washington, D.C:
    [Google Scholar]
  20. Ristic M. 1990 Current strategies in research on ehrlichiosis. 136–153 Williams J. C., Kakoma I.ed Ehrlichiosis: a vector-borne disease of animals and humans Kluwer Academic Publishers; Boston:
    [Google Scholar]
  21. Ristic M., Holland C. J., Dawson J. E., Sessions J., Palmer J. 1986; Diagnosis of equine monocytic ehrlichiosis (Potomac horse fever) by indirect immunofluorescence. J. Am. Vet. Med. Assoc. 189:39–46
    [Google Scholar]
  22. Ristic M., Huxsoll D. L. 1984 Ehrlichieae. 704–709 Krieg N. R., Holt J. G.ed Bergey’s manual of systematic bacteriology 1 The Williams & Wilkins Co.; Baltimore:
    [Google Scholar]
  23. Ristic M., Huxsoll D. L., Tachibana N., Rapmund G. 1981; Evidence of a serologic relationship between Ehrlichia canis and Ehrlichia sennetsu. Am. J. Trop. Med. Hyg. 30:1324–1328
    [Google Scholar]
  24. Shankarappa B. 1990 Ph.D. dissertation University of Maryland; College Park:
  25. Shankarappa B., Dutta S. K. 1989; Production and characterization of monoclonal antibodies to Ehrlichia risticii. Am. J. Vet. Res. 50:1145–1149
    [Google Scholar]
  26. Stephenson E. H. 1990 Experimental ehrlichiosis in nonhuman primates. 93–99 Williams J. C., Kakoma I.ed Ehrlichiosis: a vector-borne disease of animals and humans Kluwer Academic Publishers; Boston:
    [Google Scholar]
  27. Tachibana N. 1986 Sennetsu fever: the disease, diagnosis, and treatment. 205–208 Leive L.ed Microbiology—1986 American Society for Microbiology; Washington, D.C:
    [Google Scholar]
  28. Weiss E. 1982; The biology of Rickettsiae. Annu. Rev. Microbiol. 36:345–370
    [Google Scholar]
  29. Weiss E., Coolbaugh J. C., Williams J. C. 1975; Separation of viable Rickettsia typhi from yolk sac and L cell host components by Renografin density gradient centrifugation. Appl. Microbiol. 30:456–463
    [Google Scholar]
  30. Weiss E., Dasch G. A., Kang Y., Westfall H. N. 1988; Substrate utilization by Ehrlichia sennetsu and Ehrlichia risticii separated from host constituents by Renografin gradient centrifugation. J. Bacteriol. 170:5012–5017
    [Google Scholar]
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