1887

Abstract

Antigenic and genomic relatedness among , and was analyzed by enzyme-linked immunosorbent assay, Western blotting (immunoblotting) and DNA-DNA hybridization. and were serologically related, and their Western blot antigen profiles were nearly identical. Two antigens of corresponding to the 28- and 51-kDa antigens of were apparently larger than the antigens, and the 55-kDa antigen of appeared to be unique to this species. The 110-, 70-, and 44-kDa antigens of these two species were identical, as determined by the use of monospecific antibodies. DNA homology between these two species was high. On the other hand, was antigenically least reactive with the antisera to and . However, a dog convalescent-stage antiserum recognized antigens in the other two species which were different from those recognized by their homologous antisera. Similarly, homology between the DNA of and the DNAs of the other two species was very minimal. These results indicate that and are closely related both at the genomic and antigenic levels and that the relationship of these two species with is minimal.

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/content/journal/ijsem/10.1099/00207713-42-1-127
1992-01-01
2019-10-22
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http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-42-1-127
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