1887

Abstract

Actinomycete ribosomal protein AT-L30 exhibits electrophoretic mobility that is specific for each genus. On the basis of this fact, we analyzed ribosomal AT-L30 proteins from 26 type strains of species belonging to the genera and . The electrophoretic mobilities of AT-L30 preparations from these strains, as determined by two-dimensional polyacrylamide gel electrophoresis, revealed that they could be divided into two groups, one group with relative electrophoretic mobilities of 14.0 to 41.5 and another group with relative electrophoretic mobilities of -6.5 to 0. The first group corresponded to the genus , and the second group corresponded to the genus . Partial amino acid sequencing of AT-L30 preparations from several strains proved that we were indeed dealing with the specified protein homologous to ribosomal protein L30 of . Our results strongly supported the conclusions of previous work and thus proved the efficacy of ribosomal protein analysis as a novel approach for taxonomy of actinomycetes.

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1991-04-01
2022-09-25
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References

  1. Adoutte-Panvier A., Davies J. E., Gritz L. R., Littlewood B. S. 1980; Studies of ribosomal proteins of yeast species and their hybrids: gel electrophoresis and immunochemical cross-reactions. Mol. Gen. Genet. 179:273–282
    [Google Scholar]
  2. Bock A. 1985; Analysis of ribosomal proteins by two-dimensional gel electrophoresis. Methods Microbiol. 18:109–122
    [Google Scholar]
  3. Fowler V. J., Ludwig W., Stackebrandt E. 1985 Ribonucleic acid cataloguing in bacterial systematics: the phylogeny of Actinomadura. 17–40 Goodfellow M., Minnikin D. E.ed Chemical methods in bacterial systematics Academic Press, Inc. (London), Ltd.; London:
    [Google Scholar]
  4. Geisser M., Tischendorf G. W., Stôffler G. 1973; Comparative immunological and electrophoretic studies on ribosomal proteins of Bacillaceae. Mol. Gen. Genet. 127:129–145
    [Google Scholar]
  5. Geisser M., Tischendorf G. W., Stôffler G., Wittmann H. G. 1973; Immunological and electrophoretical comparison of ribosomal proteins from eight species belonging to Entero-bacteriaceae. Mol. Gen. Genet. 127:111–128
    [Google Scholar]
  6. Goff V. L., Begueret J. 1984; Immunological comparison of individual ribosomal proteins in six species of genus Podospora. Mol. Gen. Genet. 193:143–148
    [Google Scholar]
  7. Goodfellow M. 1989 Suprageneric classification of actinomycètes. 2333–2339 Williams S. T., Sharpe M. E., Holt J. G.ed Bergey’s manual of systematic bacteriology 4 The Williams & Wilkins Co.; Baltimore:
    [Google Scholar]
  8. Goodfellow M. 1989 Maduromycetes. 2509–2551 Williams S. T., Sharpe M. E., Holt J. G.ed Bergey’s manual of systematic bacteriology 4 The Williams & Wilkins Co.; Baltimore:
    [Google Scholar]
  9. Greiner-Mai E., Kroppenstedt R. M., Korn-Wendisch F., Kutzner H. J. 1987; Morphological and biochemical characterization and emended descriptions of thermophilic actinomycète species. Syst. Appl. Microbiol. 9:97–109
    [Google Scholar]
  10. Hardy S. J. S., Kurland C. G., Voynow P., Mora G. 1969; The ribosomal proteins of Escherichia coli. I. Purification of the 30S ribosomal proteins. Biochemistry 8:2897–2905
    [Google Scholar]
  11. Hori H., Higo K., Osawa S. 1977; The rates of evolution in some ribosomal components. J. Mol. Evol. 9:191–201
    [Google Scholar]
  12. Hori H., Lim B. L., Ohama T., Kumazaki T., Osawa S. 1985 Evolution of organisms deduced from 5S rRNA sequences. 324–335 Ohta T., Aoki K.ed Population genetics and molecular evolution Japan Scientific Society Press/Elsevier; Tokyo:
    [Google Scholar]
  13. Itoh T., Kudo T., Seino A. 1987; Chemotaxonomic studies on new genera of actinomycètes proposed in Chinese paper. Actinomycetologica 1:43–59
    [Google Scholar]
  14. Kaltschmidt E., Wittmann H. G. 1970; Ribosomal proteins. VII. Two-dimensional polyacrylamide gel electrophoresis for finger-printing of ribosomal proteins. Anal. Biochem 36:401–412
    [Google Scholar]
  15. Kroppenstedt R. M., Stackebrandt E., Goodfellow M. 1990; Taxonomic revision of the actinomycète genera Actinomadura and Microtetraspora. Syst. Appl. Microbiol. 13:148–160
    [Google Scholar]
  16. Mikulik K., Janda I., Weiser J., Jiranova A. 1982; Ribosomal proteins of Streptomyces aureofaciens producing tetracycline. Biochim. Biophys. Acta 699:203–210
    [Google Scholar]
  17. Miyadoh S., Amano S., Shomura T. 1989 Taxonomic relationship between Actinomadura atramentaria and “Parvopolyspora pallida,”. 5–8 Koyama Y.ed Trends in actinomycetology in Japan Society for Actinomyces; Tokyo:
    [Google Scholar]
  18. Miyadoh S., Anzai H., Amano S., Shomura T. 1989; Actinomadura malachitica and Microtetraspora viridis are synonyms, and should be transferred as Actinomadura viridis comb. nov.. Int. J. Syst. Bacteriol. 39:152–158
    [Google Scholar]
  19. Ochi K. 1989; Heterogeneity of ribosomal proteins among Streptomyces species and its application to identification. J. Gen. Microbiol. 135:2635–2642
    [Google Scholar]
  20. Ochi K. 1989; Taxonomic characterization of Streptomyces lavendulae by electrophoretic analysis of ribosomal proteins. Actinomycetologica 3:111–114
    [Google Scholar]
  21. Ochi K. 1990; Streptomyces relC mutants with an altered ribosomal protein ST-L11 and genetic analysis of a Streptomyces griseus relC mutant. J. Bacteriol. 172:4008–4016
    [Google Scholar]
  22. Ochi K. Manuscript in preparation
  23. Poschner J., Kroppenstedt R. M., Fischer A., Stackebrandt E. 1985; DNA-DNA reassociation and chemotaxonomic studies on Actinomadura, Microbispora, Microtetraspora, Micropolyspora and Nocardiopsis. Syst. Appl. Microbiol. 6:264–270
    [Google Scholar]
  24. Quiros L. M., Parra F., Hardisson C., Salas J. A. 1989; Structural and functional analysis of ribosomal subunits from vegetative mycelium and spores of Streptomyces antibioticus. J. Gen. Microbiol. 135:1661–1670
    [Google Scholar]
  25. Ritter E., Wittmann-Liebold B. 1975; The primary structure of protein L30 from Escherichia coli ribosomes. FEBS Lett. 60:153–155
    [Google Scholar]
  26. Stackebrandt E., Woese C. R. 1981; Towards a phylogeny of the actinomycètes and related organisms. Curr. Microbiol. 4:197–202
    [Google Scholar]
  27. Wittmann H. G. 1976; Structure, function and evolution of ribosomes. Eur. J. Biochem. 61:1–13
    [Google Scholar]
  28. Wittmann H. G. 1982; Components of bacterial ribosomes. Annu. Rev. Biochem. 51:155–183
    [Google Scholar]
  29. Wittmann H. G. 1983; Architecture of prokaryotic ribosomes. Annu. Rev. Biochem. 52:35–65
    [Google Scholar]
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