gen. nov., sp. nov., an Obligate Intracellular Bacterial Parasite of Small Free-Living Amoebae Free

Abstract

A new genus and species of obligate intracellular bacterial parasite of small free-living amoebae is described. This bacterium causes fatal infections in amoebae belonging to the group. It does not grow on any artificial substrate deprived of living amoeba cells. The entry of the bacterium into a host occurs by phagocytosis, but growth occurs in the cytoplasm, not in phagosomes. This parasite is readily distinguished from other kinds of previously recognized bacteria that live within amoeba cells on the basis of its host cell lytic activity. The bacterium is a gram-negative, short rod with tapered ends. It multiplies intracellularly by a transverse central pinching-off process. Cells are motile by means of a polar tuft of flagella. The bacterium is surrounded by a distinct multilayered cell envelope with a chemtype A1γ peptidoglycan. The peptidoglycan is unique in its high content of glucosamine residues with free amino groups. The DNA base composition of this organism is 43 mol% guanine plus cytosine. The name gen. nov., sp. nov. is proposed for this bacterium. The type strain of is strain L2 (= PCM 2298).

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1991-01-01
2024-03-28
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References

  1. Ball G. H. 1969 Organisms living on and in Protozoa,. 565–718 Chen T.-T.ed Research in protozoology 3 Pergamon Press; London:
    [Google Scholar]
  2. Band R. N. 1959; Nutritional and related biological studies on the free-living soil amoeba Hartmannella rhysodes. J. Gen. Microbiol. 20:80–95
    [Google Scholar]
  3. Buchanan R. E., Gibbons N. E.ed 1974 Bergey’s manual of determinative bacteriology. , 8th. The Williams & Wilkins Co.; Baltimore:
    [Google Scholar]
  4. Chapman-Andresen C. 1971; Biology of the large amoebae. Annu. Rev. Microbiol. 25:27–18
    [Google Scholar]
  5. Conn H. J., Cohen B., Fennison M. W., McClung L. S., Riker A. J. 1951 Staining methods, p. IV51-3-IV51-24. Manual of methods for pure culture study of bacteria Society of American Bacteriologists; Geneva, N.Y:
    [Google Scholar]
  6. Dolphin W. D. 1976; Effect of glucose on glycine requirement of Acanthamoeba castellanii. J. Protozool. 23:455–457
    [Google Scholar]
  7. Drozanski W. 1956; Fatal bacterial infection in soil amoebae. Acta Microbiol. Pol. 5:315–317
    [Google Scholar]
  8. Drozanski W. 1963; Studies of intracellular parasites of free-living amoebae. Acta Microbiol. Pol. 12:3–8
    [Google Scholar]
  9. Drozanski W. 1963; Observation on intracellular infection of amoebae by bacteria. Acta Microbiol. Pol. 12:9–24
    [Google Scholar]
  10. Drozanski W. 1965; Fatal bacterial infection of small free-living amoebae and related organisms. Abstr. Second Int. Conf. Protozool., London, 1965. 254–255
  11. Drozanski W., Chmielewski T. 1979; Electron microscopic studies of Acanthamoeba castellanii infected with obligate intracellular bacterial parasite. Acta Microbiol. Pol. 28:123–133
    [Google Scholar]
  12. Drozanski W., Drozanska D., Wicmska M. 1984; The cell wall of the obligate intracellular bacterial parasite of small free-living amoebae. I. Morphology and chemical composition of the rigid layer and peptidoglycan. Acta Microbiol. Pol. 33:195–206
    [Google Scholar]
  13. Drozanski W., Madra L., Chmielewski T., Schlecht S., Golecki J. R. 1990; Evidence for the presence of N-unsubstituted glucosamine residues in the peptidoglycan of Sarcobium lyticum- an intracellular bacterial parasite of amoebae. Syst. Appl. Microbiol. 13:220–226
    [Google Scholar]
  14. Gimenez D. F. 1964; Staining rickettsiae in yolk-sac cultures. Stain Technol. 39:135–140
    [Google Scholar]
  15. Hall J., Voelz H. 1985; Bacterial endosymbionts of Acanthamoeba sp. J. Protozool. 71:89–95
    [Google Scholar]
  16. Jeon K. W. 1980 Symbiosis of bacteria with amoeba. 245–262 Cook C. B., Pappas P., Rudolph E.ed Cellular interactions in symbiotic and parasitic relationships Ohio State University Press; Columbus:
    [Google Scholar]
  17. Jeon K. W., Jeon M. S. 1976; Endosymbiosis in amoebae: recently established endosymbionts have become required cytoplasmic components. J. Cell. Physiol. 89:337–344
    [Google Scholar]
  18. Jeon K. W., Lorch I. J. 1967; Unusual intracellular bacterial infection in large free-living amoebae. Exp. Cell. Res. 48:236–240
    [Google Scholar]
  19. Lapage S. P., Sneath P. H. A., Lessel E. F., Skerman V. B. D., Seeliger H. P. R., Clark W. A.ed 1975 International code of nomenclature of bacteria. 1975 Revision. American Society for Microbiology; Washington, D.C:
    [Google Scholar]
  20. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J. Mol. Biol. 3:208–218
    [Google Scholar]
  21. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J. Mol. Biol. 5:109–118
    [Google Scholar]
  22. Norris J. R., Swain H. 1971 Staining bacteria. 105–134 Norris J. R., Ribbons D. W.ed Methods in microbiology 5A Academic Press, Inc.; New York:
    [Google Scholar]
  23. Proca-Ciobanu M. D., Lupuscu G., Petrovici A., Ionescu M. D. 1975; Electron microscopic studies of phathogenic Acanthamoeba castellanii strain. The presence of bacterial endosymbionts. Int. J. Parasitol. 5:49–56
    [Google Scholar]
  24. Schlecht S., Drozanski W. 1987; Mass cultivation of an intracellular bacterial parasite (IBP) of small free-living amoebae. Syst. Appl. Microbiol. 10:92–97
    [Google Scholar]
  25. Van Bruggen J. J. A., Stumm C. K., Kor B. Z., Vogels G. D. 1985; Endosymbiotic methanogenic bacteria of the sapropelic amoeba Mastigella. FEMS Microb. Ecol. 31:187–192
    [Google Scholar]
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