Chemotaxonomic, genetic, and phenotypic characteristics of strain VKM Ac-1250T (T = type strain), which was isolated from an association with Streptomyces galilaeus INA 5888, revealed that this actinomycete is a new Glycomyces species, for which the name Glycomyces tenuis is proposed. This new species could be differentiated from Glycomyces harbinensis and Glycomyces rutgersensis by the following characteristics: Thin hyphae (diameter, 0.15 to 0.40 μm) on the substrate mycelium, absence of aerial hyphae, major menaquinone composition [MK-9(H6), MK-10(H6), and MK-11(H6)], certain physiological properties, and high levels of susceptibility to some antibiotics and growth inhibitors. The type strain of G. tenuis is strain VKM Ac-1250 (= INA n-5888).
BurtonK.1956; A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem. J. 62:315–323
EvtushenkoL. I., JanushkeneN. A., StreshinskayaG. M., NaumovaI. B., AgreN. S.1984; Occurrence of teichoic acids in representatives of Actinomycetales. Dokl. Acad. Nauk SSSR 278:237–239 (In Russian.)
GoodfellowM., LechevalierM. P.1986 Genus Nocardia,. 1459–1471SneathP. H. A., MairN. S., SharpeM. E., HoltJ. G.edBergey’s manual of systematic bacteriology2 The Williams & Wilkins Co.; Baltimore:
GordonR. E., BarnettD. A., HanderhanJ. E., PangC. H.1974; Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. Int. J. Syst. Bacteriol. 24:54–63
LechevalierM. P., LechevalierH. A.1980 The chemotaxonomy of actinomycetes. 227–291DietzA., ThayerW. D.edActinomycete taxonomy. Society for Industrial Microbiology, special publication, no. 6 Society for Industrial Microbiology; Arlington, Va:
MeyerS. A., SchleiferK. H.1978; Deoxyribonucleic acid reassociation in the classification of coagulase-positive staphylococci. Arch. Microbiol. 117:183–188
OkamiC., SuzukiM.1958; A simple method for microscopical observation for Streptomyces and criteria of Streptomyces grouping with references to aerial structure. J. Antibiot. 11:250–253
OwenR. J., PitcherD.1985 Current methods for estimating DNA base composition and levels of DNA-DNA hybridization. 67–93GoodfellowM., MinnikinD. E.edChemical methods in bacterial systematics Academic Press, Inc. (London), Ltd.; London:
StackebrandtE., Wunner-FusslB., FowlerV. J., SchleiferK.-H.1981; Deoxyribonucleic acid homologies and ribosomal ribonucleic acid similarities among sporeforming members of the order Actinomycetales. Int. J. Syst. Bacteriol. 31:420–431
StreshinskayaG. M., NaumovaI. B., PaninaL. S.1980; Chemical composition of the cell wall of Streptomyces chrysomallus producing antibiotic auranthine. Mikrobiologiya 48:814–819 (In Russian.)
ZakharovaO. D., TerekhovaL. P., AgreN. S.1980; Production of asporogenic secondary colonies by actinomycetes and the properties of variants isolated from them. Mikrobiologiya 49:976–981 (In Russian.)