Analysis of and Related Organisms by DNA-DNA Hybridization and Restriction Endonuclease Fingerprinting Free

Abstract

The objective of this study was to determine the degree of genetic relatedness of to selected members of the family , with particular emphasis on species commonly associated with swine. Free-solution DNA-DNA hybridization studies revealed that representative strains of all 12 serotypes of formed a homogeneous group, sharing 74 to 90% sequence homology with serotype 1. All serotypes of tested demonstrated a high degree of genetic relatedness (66 to 79%) to the type species of the genus . Little homology (<20%) was detected between strains and selected spp. and spp. Since free-solution hybridization methods are technically demanding and require large amounts of highly purified DNA, restriction endonuclease fingerprinting (REF) was examined to determine whether it could be a useful taxonomic tool for classification of members of the family . REF profiles were compared, and the degree of similarity between organisms was quantitated by calculating Jaccard similarity coefficients. There was a significant positive relationship between the REF Jaccard coefficients and the DNA homology values determined from free-solution hybridization experiments.

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1991-01-01
2024-03-28
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References

  1. Biberstein E. L. 1981 Haemophilus-Pasteurella-Actinobacillus: their significance in veterinary medicine. 61–73 Kilian M., Frederiksen W., Biberstein E. L.ed Haemophilus, Pasteurella, and Actinobacillus Academic Press, Inc. (London), Ltd.; London:
    [Google Scholar]
  2. Borr J. 1989 M.S. thesis University of Guelph; Guelph, Ontario, Canada:
  3. Bradbury W. C., Murray R. G. E., Mancini C., Morris V. L. 1985; Bacterial chromosomal restriction endonuclease analysis of the homology of Bacteroides species. J. Clin. Microbiol. 21:24–28
    [Google Scholar]
  4. Christiansen C., Hansen E., Friis-Moller A. 1981 Homology between DNA from selected strains of the genera Pasteurella, Actinobacillus, and Haemophilus,. 158–160 Kilian M., Frederiksen W., Biberstein E. L.ed Haemophilus, Pasteurella, and Actinobacillus Academic Press, Inc., (London), Ltd.; London:
    [Google Scholar]
  5. Colwell R. R., Austin B. 1981 Numerical taxonomy. 444–449 Gerhardt P., Murray R. G. E., Costilow R. N., Nester E. W., Wood W. A., Krieg N. R., Phillips G. B.ed Manual of methods for general bacteriology American Society for Microbiology; Washington, D.C:
    [Google Scholar]
  6. Crosa J. H., Brenner D. J., Falkow S. 1973; Use of a single-strand specific nuclease for analysis of bacterial and plasmid deoxyribonucleic acid homo- and heteroduplexes. J. Bacteriol. 115:904–911
    [Google Scholar]
  7. De Ley J., Mannheim W., Mutters R., Piechulla K., Tytgat R., Segers P., Bisgaard M., Frederiksen W., Hinz K.-H., Vanhouke M. 1990; Inter- and intrafamilial similarities of rRNA cistrons of the Pasteurellaceae. Int. J. Syst. Bacteriol. 40:126–137
    [Google Scholar]
  8. Frey J., Nicolet J. 1990; Hemolysin patterns of Actinobacillus pleuropneumoniae. J. Clin. Microbiol. 28:232–236
    [Google Scholar]
  9. Gonzales H. F., Bingham D. P. 1983; Genetic relatedness of Haemophilus somnus to select genera of bacteria. Am. J. Vet. Res. 44:1793–1795
    [Google Scholar]
  10. Grimont P. A. D., Popoff M. Y., Grimont F., Coynault C., Lemelin M. 1980; Reproducibility and correlation study of three deoxyribonucleic acid hybridization procedures. Curr. Microbiol. 4:325–330
    [Google Scholar]
  11. Johnson J. L. 1981 Genetic characterization. 450–472 Gerhardt P. C., Murray R. G. E., Costilow R. N., Nester E. W., Wood W. A., Krieg N. R., Phillips G. B.ed Manual of methods for general bacteriology American Society for Microbiology; Washington, D.C:
    [Google Scholar]
  12. Kielstein V. P., Flossmann K. K., Rohrmann B., Bocklisch H. 1985; Genotypische Untersuchungen an Erregem der haemorrhagischnekrotisierenden Pleuropneumonie des Schweines. Zentralbl. Veterinaermed. Reihe B 32:93–100
    [Google Scholar]
  13. Kilian M. 1976; A taxonomic study of the genus Haemophilus, with the proposal of a new species. J. Gen. Microbiol. 93:9–62
    [Google Scholar]
  14. Kilian M., Nicolet J., Biberstein E. L. 1978; Biochemical and serological characterization of Haemophilus pleuropneumoniae (Matthews and Pattison 1961) Shope 1964 and proposal of a neotype strain. Int. J. Syst. Bacteriol. 28:20–26
    [Google Scholar]
  15. Kowalski D., Kroeker W. D., Laskowski M. 1976; Mung bean nuclease I. Physical, chemical, and catalytic properties. Biochemistry 15:4457–4462
    [Google Scholar]
  16. Kristiansen B.-E., Sørensen B., Bjorvatn B., Falk E. S., Fosse E., Bryn K., Frøholm L. O., Gaustad P., Bøvre K. 1986; An outbreak of group B meningococcal disease: tracing the causative strain of Neisseria meningitidis by DNA fingerprinting. J. Clin. Microbiol. 23:764–767
    [Google Scholar]
  17. Kroeker W. D., Kowalski D., Laskowski M. 1978; Mung bean nuclease I. Terminally directed hydrolysis of native DNA. Biochemistry 15:4463–4467
    [Google Scholar]
  18. Maclnnes J. I., Borr J. D. 1990; The family Pasteurellaceae’. modem approaches to taxonomy. Can. J. Vet. Res 54:S6–S11
    [Google Scholar]
  19. Maclnnes J. I., Borr J. D., Massoudi M., Rosendal S. 1990; Analysis of southern Ontario Actinobacillus (Haemophilus) pleuropneumoniae isolates by restriction endonuclease fingerprinting. Can. J. Vet. Res. 54:244–250
    [Google Scholar]
  20. Maclnnes J. I., Rosendal S. 1987; Analysis of major antigens of Haemophilus (Actinobacillus) pleuropneumoniae and related organisms. Infect. Immun. 55:1626–1634
    [Google Scholar]
  21. Maclnnes J. L, Trust T. J., Crosa J. H. 1979; DNA relationships among members of the genus Aeromonas. Can. J. Microbiol. 25:579–586
    [Google Scholar]
  22. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory; Cold Spring Harbor, N.Y:
    [Google Scholar]
  23. Maniatis T., Jeffery A., van Desande H. 1975; Chain length determination of small double-stranded and single-stranded DNA molecules by polyacrylamide gel electrophoresis. Biochemistry 14:3787–3794
    [Google Scholar]
  24. Mannheim W., Carter G. R., Kilian M., Biberstein E. L., Phillips J. E. 1984 Family III Pasteurellaceae,. 550–575 Krieg N. R., Holt J. G.ed Bergey’s manual of systematic bacteriology I The Williams & Wilkins Co.; Baltimore:
    [Google Scholar]
  25. McClenaghan M., Herring A. J., Aitken I. D. 1984; Comparison of Chlamydia psittaci isolates by DNA restriction endonuclease analysis. Infect. Immun. 45:384–389
    [Google Scholar]
  26. Morozumi T., Pauli U., Braun R., Nicolet J. 1986; Deoxyribonucleic acid relatedness among strains of Haemophilus parasuis and other Haemophilus spp. of swine origin. Int. J. Syst. Bacteriol. 36:17–19
    [Google Scholar]
  27. Newberry A., Maclnnes J. I. Unpublished data
  28. Nicolet J. 1988 Haemophilus infections. 426–436 Leman A. D., Straw B., Glock R. D., Mengeling W. L., Penny R. H. C., Scholl E.ed Diseases of swine, 6th. Iowa State University Press; Ames:
    [Google Scholar]
  29. Nielsen R. 1988; Seroepidemiology of Actinobacillus pleuropneumoniae. Can. Vet. J. 29:580–582
    [Google Scholar]
  30. Niven D. F., O’Reilly T. 1990; Significance of V-factor dependency in the taxonomy of Haemophilus species and related organisms. Int. J. Syst. Bacteriol. 40:1–4
    [Google Scholar]
  31. O’Reilly T., Niven D. F. 1986; Tryptone-yeast extract broth as a culture medium for Haemophilus pleuropneumoniae and Haemophilus parasuis to be used as challenge inocula. Can. J. Vet. Res. 50:441–443
    [Google Scholar]
  32. Pohl S. 1981 DNA relatedness among members of Haemophilus, Pasteurella, and Actinobacillus,. 245–253 Kilian M., Frederiksen W., Biberstein E. L.ed Haemophilus, Pasteurella, and Actinobacillus Academic Press, Inc. (London), Ltd.; London:
    [Google Scholar]
  33. Pohl S., Bertschinger H. U., Frederiksen W., Mannheim W. 1983; Transfer of Haemophilus pleuropneumoniae and the Pasteurella haemolytica-like organism causing porcine necrotic pleuropneumonia to the genus Actinobacillus (Actinobacillus pleuropneumoniae comb, nov.) on the basis of phenotypic and DNA relatedness. Int. J. Syst. Bacteriol. 33:510–514
    [Google Scholar]
  34. Rapp V. J., Munson R. S., Ross R. F. 1986; Outer membrane protein profiles of Haemophilus pleuropneumoniae. Infect. Immun. 52:414–420
    [Google Scholar]
  35. Rapp V. J., Ross R. F., Young T. F. 1985; Characterization of Haemophilus spp. isolated from healthy swine and evaluation uf cross-reactivity of complement-fixing antibodies to Haemophilus pleuropneumoniae and Haemophilus taxon “minor group.”. J. Clin. Microbiol. 22:945–950
    [Google Scholar]
  36. Rapp V. J., Ross R. F., Zimmermann Erickson B. 1985; Serotyping of Haemophilus pleuropneumoniae by rapid slide agglutination and indirect fluorescent antibody tests in swine. Am. J. Vet. Res. 46:185–192
    [Google Scholar]
  37. Rosendal S., Boyd D. A., Gilbride K. A. 1985; Comparative virulence of porcine Haemophilus pleuropneumoniae bacteria. Can. J. Comp. Med. 46:68–74
    [Google Scholar]
  38. Rosendal S., Mittal K. R. 1985; Serological cross-reactivity between a porcine Actinobacillus strain and Haemophilus pleuropneumoniae. Can. J. Comp. Med. 49:164–170
    [Google Scholar]
  39. Sammons D. W., Adams L. D., Nishizawa E. E. 1981; Ultrasensitive silver-based color staining of polypeptides in polyacrylamide gels. Electrophoresis 2:135–141
    [Google Scholar]
  40. Scherer S., Stevens D. A. 1987; Application of DNA typing methods to epidemiology and taxonomy of Candida species. J. Clin. Microbiol. 25:675–679
    [Google Scholar]
  41. Sebunya T. N. K., Saunders J. R. 1983; Haemophilus pleuropneumoniae infection in swine: a review. J. Am. Vet. Med. Assoc. 15:1331–1337
    [Google Scholar]
  42. Smart N. L., Miniats O. P., Machines J. I. 1988; Analysis of Haemophilus parasuis isolates from southern Ontario swine by restriction endonuclease fingerprinting. Can. J. Vet. Res. 52:319–324
    [Google Scholar]
  43. Sneath P. H. A., Johnson R. 1973; Numerical taxonomy of Haemophilus and related bacteria. Int. J. Syst. Bacteriol 23:405–418
    [Google Scholar]
  44. Sneath P. H. A., Stevens M. 1990; Actinobacillus rossii sp. nov., Actinobacillus seminis sp. nov., nom. rev., Pasteurella bettii sp. nov., Pasteurella lymphangitidis sp. nov., Pasteurella mairi sp. nov., and Pasteurella trehalosi sp. nov. Int. J. Syst. Bacteriol. 40:148–153
    [Google Scholar]
  45. Srivastava M. S., Carter E. M. 1983 An introduction to applied multivariate statistics. North-Holland Publishing Co.; New York:
    [Google Scholar]
  46. Stahl M., Molin G., Persson A., Ahrne S., Stahl S. 1990; Restriction endonuclease patterns and multivariate analysis as a classification tool for Lactobacillus spp. Int. J. Syst. Bacteriol. 40:189–193
    [Google Scholar]
  47. Steel R. G. D., Torrie J. H. 1980 Principles and procedures of statistics. McGraw-Hill Book Co.; New York:
    [Google Scholar]
  48. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E., Stackenbrandt E., Starr M. P., Truper H. G. 1987; Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int. J. Syst. Bacteriol. 37:463–464
    [Google Scholar]
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