1887

Abstract

The objective of this study was to determine the degree of genetic relatedness of to selected members of the family , with particular emphasis on species commonly associated with swine. Free-solution DNA-DNA hybridization studies revealed that representative strains of all 12 serotypes of formed a homogeneous group, sharing 74 to 90% sequence homology with serotype 1. All serotypes of tested demonstrated a high degree of genetic relatedness (66 to 79%) to the type species of the genus . Little homology (<20%) was detected between strains and selected spp. and spp. Since free-solution hybridization methods are technically demanding and require large amounts of highly purified DNA, restriction endonuclease fingerprinting (REF) was examined to determine whether it could be a useful taxonomic tool for classification of members of the family . REF profiles were compared, and the degree of similarity between organisms was quantitated by calculating Jaccard similarity coefficients. There was a significant positive relationship between the REF Jaccard coefficients and the DNA homology values determined from free-solution hybridization experiments.

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/content/journal/ijsem/10.1099/00207713-41-1-121
1991-01-01
2019-10-19
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http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-41-1-121
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