1887

Abstract

Strain 831-C4 (T = type strain), isolated from the surface of lettuce plants () obtained from a retail food market, was shown to be a sterol-requiring mollicute. Morphological examination of this organism by electron and dark-field microscopic techniques showed that it consists of small, nonhelical, nonmotile, pleomorphic coccoid cells, with individual cells surrounded by a single cytoplasmic membrane. No evidence of a cell wall was observed. The organism grew rapidly in all conventional culture medium formulations for mollicutes in either aerobic or anaerobic environments. The optimum temperature for growth was 30°C, but multiplication occurred at 18 to 37°C. Strain 831-C4 catabolized glucose, but hydrolysis of arginine or urea could not be demonstrated. The genome size of strain 831-C4 was determined to be about 569 megadaltons, while the base composition (guanine-plus-cytosine content) of the DNA was 30.0 mol%. Recent studies in which we compared the 16S rRNA sequences of strain 831-C4 with those of more than 40 other mollicutes indicated that this organism is phylogenetically related to the clade. Strain 831-C4 was serologically unrelated to the type strains of previously described species and to 18 other unclassified sterol-requiring isolates cultivated from various animal, plant, or insect sources. Strain 831-C4 (= ATCC 49193) is the type strain of sp. nov.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-40-2-138
1990-04-01
2022-12-09
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/40/2/ijsem-40-2-138.html?itemId=/content/journal/ijsem/10.1099/00207713-40-2-138&mimeType=html&fmt=ahah

References

  1. Aluotto B. B., Wittier R. G., Williams C. O., Faber J. E. 1970; Standardized bacteriologic techniques for characterization of Mycoplasma species. Int. J. Syst. Bacteriol. 20:35–58
    [Google Scholar]
  2. Carle P., Bové J. M. 1983; Genome size determination. Methods Mycoplasmol. 1:309–311
    [Google Scholar]
  3. Carle P., Saillard C., Bové J. M. 1983; DNA extraction and purification. Methods Mycoplasmol. 1:295–299
    [Google Scholar]
  4. Carle P., Saillard C., Bové J. M. 1983; Determination of guanine plus cytosine content of DNA. Methods Mycoplasmol. 1:301–308
    [Google Scholar]
  5. Clark T. B., Tully J. G., Rose D. L., Henegar R., Whitcomb R. F. 1986; Acholeplasmas and similar nonsterol-requiring mollicutes from insects: missing link in microbial ecology. Curr. Microbiol. 13:11–16
    [Google Scholar]
  6. Clyde W. A. Jr. 1983; Growth inhibition tests. Methods Mycoplasmol. 1:405–410
    [Google Scholar]
  7. Desantis D., Tryon V. V., Pollack J. D. 1989; Metabolism of mollicutes: the Embden-Meyerhof-Pamas pathway and the hexose monophosphate shunt. J. Gen. Microbiol. 135:683–691
    [Google Scholar]
  8. Eden-Green S., Tully J. G. 1979; Isolation of Acholeplasma spp. from coconut palms affected by lethal yellowing disease in Jamaica. Curr. Microbiol. 2:311–316
    [Google Scholar]
  9. Edward D. G. ff. 1947; A selective medium for pleuropneumonia-like organisms. J. Gen. Microbiol. 1:238–243
    [Google Scholar]
  10. Freundt E. A. 1983; Film and spot production. Methods Mycoplasmol. 1:373–374
    [Google Scholar]
  11. Gardella R. S., Del Giudice R. A. 1983; Hemagglutination, hemadsorption, and hemolysis. Methods Mycoplasmol. 1:379384
    [Google Scholar]
  12. Gardella R. S., Del Giudice R. A., Tully J. G. 1983; Immunofluorescence. Methods Mycoplasmol. 1:431–439
    [Google Scholar]
  13. International Committee on Systematic Bacteriology Subcommittee on the Taxonomy of Mollicutes 1979; Proposal of minimal standards for descriptions of new species of the class Mollicutes. Int. J. Syst. Bacteriol. 29:172–180
    [Google Scholar]
  14. McCoy R. E., Basham H. G., Tully J. G., Rose D. L., Carle P., Bové J. M. 1984; Acholeplasma florum: a new species isolated from plants. Int. J. Syst. Bacteriol. 34:11–15
    [Google Scholar]
  15. McCoy R. E., Williams D. S., Thomas D. L. 1979 Isolation of mycoplasmas from flowers. 75–80 McCoy R. E., Su H.ed Proceedings of the Republic of China-United States Cooperative Science Seminar Symposium Series I National Science Council; Taipei, Taiwan:
    [Google Scholar]
  16. Senterfit L. B. 1983; Preparation of antigens and antisera. Methods Mycoplasmol. 1:401–404
    [Google Scholar]
  17. Somerson N. L., Kocka J. P., Rose D., Del Giudice R. A. 1982; Isolation of acholeplasmas and a mycoplasma from vegetables. Appl. Environ. Microbiol. 43:412–417
    [Google Scholar]
  18. Tully J. G. 1983; Cloning and filtration techniques for mycoplasmas. Methods Mycoplasmol. 1:173–177
    [Google Scholar]
  19. Tully J. G. 1983; Tests for digitonin sensitivity and sterol requirement. Methods Mycoplasmol. 1:355–362
    [Google Scholar]
  20. Tully J. G. 1984 The family Acholeplasmataceae, genus Acholeplasma. 781–787 Krieg N. R., Holt J. M.ed Bergey’s manual of systematic bacteriology 1 The Williams & Wilkins Co.; Baltimore:
    [Google Scholar]
  21. Tully J. G. 1989 Class Mollicutes: new perspectives from plant and arthropod studies. 1–31 Whitcomb R. F., Tully J. G.ed The mycoplasmas 5 Academic Press; Inc., New York:
    [Google Scholar]
  22. Tully J. G., Rose D. L., Carle P., Bové J. M., Hackett K. J., Whitcomb R. F. 1988; Acholeplasma entomophilum sp. nov. from gut contents of a wide range of host insects. Int. J. Syst. Bacteriol. 38:164–167
    [Google Scholar]
  23. Tully J. G., Rose D. L., Hackett K. J., Whitcomb R. F., Carle P., Bove J. M., Colflesh D. E., Williamson D. L. 1989; Mycoplasma ellychniae sp. nov., a sterol-requiring mollicute from the firefly beetle Ellychnia corrusca. Int. J. Syst. Bacteriol. 39:284–289
    [Google Scholar]
  24. Tully J. G., Rose D. L., McCoy R. E., Carle P., Bove J. M., Whitcomb R. F., Weisburg W. G. 1990; Mycoplasma melaleucae sp. nov., a sterol-requiring mollicute from flowers of several tropical plants. Int. J. Syst. Bacteriol. 40:143–147
    [Google Scholar]
  25. Tully J. G., Rose D. L., Whitcomb R. F., Hackett K. J., Clark T. B., Henegar R. B., Clark E., Carle P., Bove J. M. 1987; Characterization of some new insect-derived acholeplasmas. Isr. J. Med. Sci. 23:699–703
    [Google Scholar]
  26. Weisburg W. G., Tully J. G., Rose D. L., Petzel J. P., Oyaizu H., Yang D., Mandelco L., Sechrest J., Lawrence T. G., Van Etten J., Maniloff J., Woese C. R. 1989; A phylogenetic analysis of the mycoplasmas: basis for their classification. J. Bacteriol. 171:6455–6467
    [Google Scholar]
  27. Whitcomb R. F. 1983; Culture media for spiroplasmas. Methods Mycoplasmoi. 1:147–158
    [Google Scholar]
  28. Whitcomb R. F., Tully J. G., Rose D. L., Stephens E. B., Barile M. F., Smith A. J., McCoy R. E. 1982; Wall-less prokaryotes from fall flowers in central United States and Maryland. Curr. Microbiol. 7:291–296
    [Google Scholar]
  29. Williamson D. L., Tully J. G., Rose D. L., Hackett K. J., Henegar R., Carle P., Bové J. M., Colflesh D. E., Whitcomb R. F. 1990; Mycoplasma somnilux sp. nov., Mycoplasma luminosum sp. nov., and Mycoplasma lucivorax sp. nov., new sterol-requiring moflientes from firefly beetles (Coleóptera: Lampyridae). Int. J. Syst. Bacteriol. 40:160–164
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-40-2-138
Loading
/content/journal/ijsem/10.1099/00207713-40-2-138
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error