1887

Abstract

The DNA base compositions of 78 alkaliphilic strains were determined. These strains were grouped as follows: DNA group A, guanine-plus-cytosine (G+C) content of 34.0 to 37.5 mol% (17 strains); DNA group B, G+C content of 38.2 to 40.8 mol% (33 strains); and DNA group C, G+C content of 42.1 to 43.9 mol% (28 strains). DNA group A includes the type strain of Vedder 1934. DNA-DNA hybridization studies with DNA group A strains revealed that only one strain, strain DSM 2526, exhibited a high level of DNA homology with DSM 485 (T = type strain). Neither strain DSM 485 nor any other DNA group A strain is homologous to any of the type strains with comparable base compositions. Six strains formed a distinct group containing three highly homologous strains and three strains exhibiting >50% DNA homology.

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1990-01-01
2024-03-04
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References

  1. Anderson A. 1984; Search begins for superbugs. Nature (London) 310:172–173
    [Google Scholar]
  2. Aunstrup K., Outtrup H., Andresen O., Dambmann C. 1972 Proteases from alkalophilic Bacillus species. 299–305 Terui G.ed Proceedings of the Fourth International Symposium on Fermentation Technology Society of Fermentation Technology; Osaka, Japan:
    [Google Scholar]
  3. Boyer E., Ingle M. B., Mercer G. D. 1973; Bacillus alkalophilus subsp. halodurans subsp. nov.: an alkaline-amylase-producing alkalophilic organism. Int. J. Syst. Bacteriol. 23:238–242
    [Google Scholar]
  4. Bredemann G., Werner W. 1933; Botanische Beschreibung häufiger am Buttersäureabbau beteiligter sporenbildender Bakterienspezies. Zentralbl. Bakteriol. Parasitenkd. Infektionskr. Hyg. Abt. 2 87:446–475
    [Google Scholar]
  5. Chislett M. E., Kushner D. J. 1961; A strain of Bacillus circulans capable of growing under highly alkaline conditions. J. Gen. Microbiol. 24:187–190
    [Google Scholar]
  6. Claus D., Berkeley R. C. W. 1986 Genus Bacillus Cohn 1872. 1105–1139 Sneath P. H. A.ed Bergey’s manual of systematic bacteriology 2 The Williams & Wilkins Co.; Baltimore:
    [Google Scholar]
  7. De Ley J. 1970 Molecular techniques and applications in bacterial taxonomy. 317–327 Prauser H.ed The Actinomycetales Jena, German Democratic Republic;
    [Google Scholar]
  8. De Ley J. 1978; Modem molecular methods in bacterial taxonomy: evaluation, application, prospects. 347–357Proceedings of the Fourth International Conference on Plant Pathology and Bacteriology
    [Google Scholar]
  9. Erickson R. J. 1976 Industrial applications of the bacilli: a review and prospectus. 406–419 Schlessinger D.ed Microbiology—1976 American Society for Microbiology; Washington, D. C.:
    [Google Scholar]
  10. Fahmy F. A., Flossdorf J., Claus D. 1985; The DNA base composition of the type strains of the genus Bacillus. Syst. Appl. Microbiol. 6:60–65
    [Google Scholar]
  11. Feng P. C. S., Hartman P. A. 1982; Fluorogenic assays for immediate confirmation of Escherichia coli. Appl. Environ. Microbiol. 43:1320–1329
    [Google Scholar]
  12. Flossdorf J. 1983; A rapid method for the determination of the base composition of bacterial DNA. J. Microbiol. Methods 1:305–311
    [Google Scholar]
  13. Fogarty W. M., Griffin P. J., Joyce A. M. 1974; Enzymes of Bacillus species. Proc. Biochem.JulyAugust11–24
    [Google Scholar]
  14. Fogarty W. M., Griffin P. J., Joyce A. M. 1974; Enzymes of Bacillus species. II. Proc. Biochem.September27–35
    [Google Scholar]
  15. Gibson T. 1935; The urea-decomposing microflora of soils. I. Description and classification of the organisms. Zentralbl. Bakteriol. Parasitenkd. Infektionskr. Hyg. Abt. 2 92:364–380
    [Google Scholar]
  16. Gordon R. E., Haynes W. C., Pang C. H. 1973; The genus Bacillus. U.S. Dep. Agric. Agric. Handb. 427:
    [Google Scholar]
  17. Gordon R. E., Hyde J. L. 1982; The Bacillus firmus-Bacillus lentus complex and pH 7.0 variants of some alkalophilic strains. J. Gen. Microbiol. 128:1109–1116
    [Google Scholar]
  18. Gordon R. E., Hyde J. C., Moore J. A. 1977; Bacillus firmus-Bacillus lentus: a series or one species?. Int. J. Syst. Bacteriol. 27:256–262
    [Google Scholar]
  19. Guffanti A. A., Blanco R., Benenson R. A., Krulwich T. A. 1980; Bioenergetic properties of alkaline-tolerant and alkalophilic strains of Bacillus firmus. J. Gen. Microbiol. 119:79–86
    [Google Scholar]
  20. Horikoshi K., Akiba T. 1982 Alkalophilic microorganisms, a new microbial world. Japan Scientific Societies Press; Tokyo:
    [Google Scholar]
  21. Jensen G. 1972; Bacillus derived detergent enzymes. Proc. BiochemAugust
    [Google Scholar]
  22. Jordan E. O. 1890 A report on certain species of bacteria observed in sewage. 821–844Sedge wicked A report of the biological work of the Lawrence Experiment Station, including an account of methods employed and results obtained in the microscopical and bacteriological investigation of sewage and water Report on water supply and sewerage, part 2 Massachusetts State Board of Health; Boston:
    [Google Scholar]
  23. Krych V. A., Johnson J. L., Yousten A. A. 1980; Deoxyribonucleic acid homologies among strains of Bacillus sphaericus. Int. J. Syst. Bacteriol. 30:476–484
    [Google Scholar]
  24. Kutzner H. 1976 The family Streptomycetaceae,. 2028–2090 The procaryotes 2 Springer-Verlag KG; Berlin:
    [Google Scholar]
  25. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J. Mol. Biol. 3:208–218
    [Google Scholar]
  26. Meyer A., Neide E. 1904 Zentralbl. Bakteriol. Parasitenkd. Infektionskr. Hyg. Abt. 2 12:337–352
    [Google Scholar]
  27. Morgan F. J., Adams K. R., Priest F. G. 1979; A cultural method for the detection of pullulan-degrading enzymes in bacteria and its application to the genus Bacillus. J. Appl. Bacteriol. 46:291–294
    [Google Scholar]
  28. Nakamura L. K., Swezey J. 1983; Taxonomy of Bacillus circulans Jordan 1890: base composition and reassociation of deoxyribonucleic acid. Int. J. Syst. Bacteriol. 33:46–52
    [Google Scholar]
  29. Proom H., Knight B. C. J. G. 1950; Bacillus pantothenticus (n. sp.). J. Gen. Microbiol. 4:539–541
    [Google Scholar]
  30. Rüger H.-J. 1983; Differentiation of Bacillus globisporus, Bacillus marinus comb, nov., Bacillus aminovorans, and Bacillus insolitus. Int. J. Syst. Bacteriol. 33:157–161
    [Google Scholar]
  31. Sierra G. 1957; A simple method for the detection of lipolytic activity of microorganisms and some observations on the influence of the contact between cells and fatty substrates. Antonie van Leeuwenhoek J. Microbiol. Serol. 23:15–22
    [Google Scholar]
  32. Skerman V. B. D., McGowan V., Sneath P. H. A.ed 1980 Approved lists of bacterial names. Int. J. Syst. Bacteriol. 30:225–420
    [Google Scholar]
  33. Smith N. R., Gordon R. E., Clark F. E. 1952 Aerobic sporeforming bacteria. U.S. Department of Agriculture Monograph no. 16. U.S. Department of Agriculture; Washington, D.C:
    [Google Scholar]
  34. Smith R. E. 1977; Rapid tube test for detecting fungal cellulase production. Appl. Environ. Microbiol. 33:980–981
    [Google Scholar]
  35. Sneath P. H. A. 1977; The maintenance of large numbers of strains of microorganisms, and the implication for culture collections. FEMS Microbiol. Lett. 1:333–334
    [Google Scholar]
  36. Thornley M. J. 1960; The differentiation of Pseudomonas from other gram negative bacteria on the basis of arginine metabolism. J. Appl. Bacteriol. 23:37–52
    [Google Scholar]
  37. Vedder A. 1934; Bacillus alcalophilus n. sp.; benevens enkele ervaringen met sterk alcalische voedingsbodems. Antonie van Leeuwenhoek J. Microbiol. Serol. 1:143–147
    [Google Scholar]
  38. Weisser J., Trüper H.-G. 1985; Osmoregulation in a new haloalkaliphilic Bacillus from the Wadi Natrun (Egypt). Syst. Appl. Microbiol. 6:7–11
    [Google Scholar]
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