A new Saccharopolyspora species isolated from soil collected in a sugar mill rum still is described. This organism is characterized by pale yellowish pink aerial hyphae that bear long chains of spores encased in distinctive spiny spore sheaths. Fragmentation occurs when the organism is cultured in liquid media. The new species contains meso-diaminopimelic acid, arabinose, and galactose (cell wall chemotype IV), has whole-cell sugar pattern type A, contains no mycolic acids, and contains phosphatidylcholine (phospholipid pattern type PIII). The single isolate has chemical and morphological properties consistent with the genus Saccharopolyspora. A comparison with previously described species of the genus in which we used standard techniques plus fatty acid analyses showed that this organism differs from previously described species. The name proposed for this new species is Saccharopolyspora spinosa. The type strain is strain A83543.1 (= NRRL 18395). The description is based on a single isolate.
AldersonG.1985 The application and relevance of nonhierarchic methods in bacterial taxonomy. 227–263GoodfellowM., JonesD., PriestF. G.edComputer-assisted bacterial systematics Academic Press, Inc.; New York:
BeckerB., LechevalierM. P., GordonR. E., LechevalierH. E.1964; Rapid differentiation between Norcardia and Streptomyces by paper chromatography of whole-cell hydrolysates. Appl. Microbiol. 12:421–423
GordonR. E., BarnettD. A., HanderhanJ. E., PangC.1974; Nocardia coeliaca, Nocardia autotrophica, and the Nocardin strain. Int. J. Syst. Bacteriol. 24:54–63
IwasakiA., HisakatsuI., MoriT.1979; A new broadspectrum aminoglycoside antibiotic complex, sporaricin. II. Taxonomic studies on the sporaricin producing strain Saccharopolyspora hirsuta subsp. kobensis nov. subsp. J. Antibiot. 32:180–186
KroppenstedtR. M.1985 Fatty acid and menaquinone analysis of actinomycetes and related organisms. 173–196GoodfellowM., MinnikinD. E.edChemical methods in bacterial systematics Academic Press, Inc.; New York:
LabedaD.1987; Transfer of the type strain of Streptomyces erythraeus (Waksman 1923) Waksman and Henrici 1948 to the genus Saccharopolyspora Lacey and Goodfellow 1975 as Saccharopolyspora erythraea sp. nov., and designation of a neotype strain for Streptomyces erythraeus. Int. J. Syst. Bacteriol. 37:19–22
LechevalierM. P., LechevalierH.1970; Chemical composition as a criterion in the classification of aerobic actinomycetes. Int. J. Syst. Bacteriol. 20:435–443
LechevalierM. P., PrauserH., LabedaD. P., RuanJ. S.1986; Two new genera of nocardioform actinomycetes: Amycolata gen. nov. and Amycolatopsis gen. nov. Int. J. Syst. Bacteriol. 36:29–37
LechevalierM. P., StemA. E., LechevalierH. A.1981 Phospholipids in the taxonomy of actinomycetes. 111–116SchaalK. P., PulvererG.edActinomycetes Gustav Fischer Verlag; Stuttgart, Federal Republic of Germany:
MinnikinD. E., HutchinsonI. G., CaldicottA. B.1980; Thin-layer chromatography of methanolysates of mycolic acidcontaining bacteria. J. Chromatogr. 188:221–233
United States Department of Commerce National Bureau of Standards1958 ISCC-NBS centroid color charts. Standard sample no. 2106. U.S. Department of Commerce; Washington, D.C: