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Clostridium intestinalis sp. nov. is described on the basis of five strains isolated from the feces of cattle and pigs. The isolates are aerotolerant, gram-positive, sporeforming, motile rods. They differ from all the validly described aerotolerant species of the genus Clostridium by carbohydrate fermentation pattern, guanine-plus-cytosine content of the deoxyribonucleic acid, and deoxyribonucleic acid homologies. The fermentation products from peptone-yeast extract-Fildes solution-1 % (wt/vol) glucose broth are major amounts of acetic and butyric acids. The guanine-plus-cytosine content of the deoxyribonucleic acid is 26 to 28 mol%. The type strain of Clostridium intestinalis is Catt39 (= ATCC 49213 and JCM 7506).
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