A total of 46 glutamate-degrading strains of the genus Clostridium, including 36 new isolates, were examined for deoxyribonucleic acid (DNA)-DNA homology, protein patterns produced by polyacrylamide gel electrophoresis, and physiological characteristics in order to determine the differences between Clostridium cochlearium and “Clostridium tetanomorphum.” Thirty-four newly isolated strains could be assigned to the “C. tetanomorphum” group; two strains were classified as nontoxigenic Clostridium tetani strains. The levels of DNA-DNA homology between C. cochlearium and “C. tetanomorphum” were only 10 to 19%, and the protein patterns showed that there were great differences between both groups and other glutamate-degrading species. Biochemical tests supported these results. In contrast to C. cochlearium, all “C. tetanomorphum”, strains fermented carbohydrates (at least glucose and maltose); they also produced lipase and degraded ethanolamine. Moreover, C. cochlearium, like other glutamate-degrading species, needed a higher concentration of yeast extract than “C. tetanomorphum” when it was grown on glutamate. On the basis of our results we propose C. tetanomorphum sp. nov., nom. rev. The type strain is strain NCTC 543 (= DSM 4474).
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BarkerH. A.1939; The use of glutamic acid for the isolation and identification of Clostridium cochlearium and Cl. tetanomorphum. Arch. Mikrobiol. 10:376–384
BergeyD. H.,
HarrisonF. C.,
BreedR.,
HammerB. W.,
HuntoonF. M.
(ed.) 1923Bergey's manual of determinative bacteriology, 1st ed.. The Williams & Wilkins Co.; Baltimore:
BreedR. S.,
MurrayE. G. D.,
SmithN. R.
(ed.). 1957Bergey's manual of determinative bacteriology, 7th ed..634–693 The Williams & Wilkins Co.; Baltimore:
BullochW.,
BullochW. E.,
DouglasS. R.,
HenryH.,
McIntoshJ.,
O'BrienR. A.,
RobertsonM.,
WolfC. G. L.1919; Report on the anaerobic infections of wounds and the bacteriological and serological problems arising therefrom. Med. Res. Comm. (Great Britain) Spec. Rep. Ser. 39:1–182
CatoE. P.,
GeorgeW. L.,
FinegoldS. M.1986; Genus Clostridium Prazmowski 1880, 23AL. 1141–1200 In
SneathP. H. A.,
MairN. S.,
SharpeM. E.,
HoltJ. G.
(ed.) Bergey's manual of systematic bacteriology vol. 2 The Williams & Wilkins Co.; Baltimore:
GottschalkG.,
AndreesenJ. R.,
HippeH.1981; The genus Clostridium. 1767–1803 In
StarrM. P.,
StolpH.,
TriiperH. G.,
BalowsA.,
SchlegelH. G.
(ed.) The prokaryotes Springer-Verlag; Berlin:
GottwaldM.,
HippeH.,
GottschalkG.1984; Formation of n-butanol from D-glucose by strains of the “Clostridium tetanomorphum” group. Appl. Environ. Microbiol. 48:573–576
HoldemanL. V.,
CatoE. P.,
MooreW. E. C.
(ed.) 1977Anaerobe laboratory manual, 4th ed.. Virginia Polytechnic Institute and State University; Blacksburg:
HussV. A. R.,
FestlH.,
SchleiferK. H.1983; Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst. Appl. Microbiol. 4:184–192
KoranskyJ. R.,
AllenS. D.,
DowellV. R.1978; Use of ethanol for selective isolation of sporeforming microorganisms. Appl. Environ. Microbiol. 35:762–765
MandelM.,
MarmurJ.1968; Use of ultraviolet absorbance-temperature for determining the guanine plus cytosine content of DNA. Methods Enzymol. 12:195–206
MooreW. E. C.,
HashD. E.,
HoldemanL. V.,
CatoE. P.1980; Polyacrylamide slab gel electrophoresis of soluble proteins for studies of bacterial floras. Appl. Environ. Microbiol. 39:900–907
NeuhoffV.,
StammR.,
EiblH.1985; Clear background and highly sensitive protein staining with Coomassie blue dies in polyacrylamide gels: an uptemative analysis. Electrophoresis 6:427–449
SmibertR. M.,
KriegN. R.1981; General characterization. 409–443 In
GerhardtP.,
MurrayR. G. E.,
CostilowR. N.,
NesterE. W.,
WoodW. A.,
KriegN. R.,
PhillipsG. B.
(ed.) Manual of methods for general bacteriology American Society for Microbiology; Washington, D.C.:
SmithL. D.,
HobbsG.1974; Clostridium Prazmowski, 1880, 23. 551–572 In
BuchananR. E.,
GibbonsN. E.
(ed.) Bergey's manual of determinative bacteriology, 8th ed.. The Williams & Wilkins Co.; Baltimore:
WachsmanJ. T.,
BarkerH. A.1955; The accumulation of formamide during the fermentation of histidine by Clostridium tetanomorphum. J. Bacteriol. 69:83–88
WeissN.,
SchleiferK. H.,
KandierO.1981; The peptidoglycan types of gram-positive anaerobic bacteria and their taxonomic implications. Rev. Inst. Pasteur Lyon 14:3–12