Most probable numbers for -like bacteria degrading stearate were 1.5 x 10 to 4.6 x 10, 2.4 x 10, and 4.6 x 10 cells per ml of municipal anaerobic digester sludge, swine waste lagoon sediment, and cow ruminal fluid, respectively. Strain SD2, which was isolated from digestor sludge in coculture with the H-utilizing bacterium sp. strain G-11, plus sulfate, was a long-chain saturated fatty-acid-using bacterium identified as a strain of the . Strain SD2 differed from subsp. subsp. nov. in its usually smaller size and in its ability to catabolize C to C saturated fatty acids. It differed from in not needing relatively high concentrations of Ca and in its inability to catabolize unsaturated fatty acids, such as oleic or linoleic acid. A strain SD2 coculture was further adapted to grow on crotonate and was subsequently purified without sulfate, with cells of strain G-11 only rarely seen. A 16S ribosomal ribonucleic acid sequence analysis (H. Zhao, D. Yang, C. Woese, and M. P. Bryant, manuscript in preparation) indicated that strain SD2 is phylogenetically very closely related to . Thus, we propose the name subsp. for this organism (strain DSM 4212) (T = type strain) because of its ecologically important ability to use stearate and other long-chain saturated fatty acids. Emended descriptions of the genus and species are given.


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