1887

Abstract

Abstract

Most probable numbers for bacteria degrading stearate were 1.5 x 10 to 4.6 x 10, 2.4 × 10, and 4.6 × 10 cells per ml of municipal anaerobic digester sludge, swine waste lagoon sediment, and cow ruminai fluid, respectively. Strain SD2, which was isolated from digestor sludge in coculture with the H-utilizing bacterium sp. strain G-11, plus sulfate, was a long-chain saturated fatty-acid-using bacterium identified as a strain of the genus Strain SD2 differed from subsp. subsp. nov. in its usually smaller size and in its ability to catabolize C to C saturated fatty acids. It differed from in not needing relatively high concentrations of Ca and in its inability to catabolize unsaturated fatty acids, such as oleic or linoleic acid. A strain SD2 coculture was further adapted to grow on crotonate and was subsequently purified without sulfate, with cells of strain G-11 only rarely seen. A 16S ribosomal ribonucleic acid sequence analysis (H. Zhao, D. Yang, C. Woese, and M. P. Bryant, manuscript in preparation) indicated that strain SD2 is phylogenetically very closely related to Thus, we propose the name subsp. for this organism (strain DSM 4212) (T = type strain) because of its ecologically important ability to use stearate and other long-chain saturated fatty acids. Emended descriptions of the genus and species are given.

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1989-04-01
2024-11-06
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