Tests allowing the screening of large numbers of enterobacterial strains for the presence of the glucose oxidation pathway (glucose, gluconate, and 2-ketogluconate dehydrogenases) were devised or adapted. A total of 506 strains representing 111 taxa (named species or subspecies and unnamed genomic groups) were studied. The members of the genera Budvicia, Edwardsiella, Leminorella, Providencia, and Xenorhabdus and the species Citrobacter freundii, Erwinia carnegeana, Erwinia carotovora, Erwinia chrysanthemi, Erwinia nigrifiuens, Erwinia Salicis, Moellerella wisconsensis, Proteus penneri, Proteus vulgaris, Yersinia intermedia, Yersinia pestis, Yersinia pseudotuberculosis, and Yersinia ruckeri were negative in all tests. Five species, Erwinia cypripedii, Ewingella americana, Rahnella aquatilis, Serratia marcescens (at 20°C), and Tatumella ptyseos produced 2,5-diketogluconate from glucose without a requirement for pyrroloquinoline quinone (PQQ). When PQQ was provided (required for glucose oxidation), Serratia grimesii and Serratia liquefaciens produced 2,5-diketogluconate from glucose at 20°C. Escherichia blattae had gluconate- and 2-ketogluconate dehydrogenases without glucose dehydrogenase. The members of the genera Hafnia, Obesumbacterium, and Pragia had only gluconate dehydrogenase. Other species had glucose dehydrogenase (with or without a requirement for PQQ) with or without gluconate dehydrogenase. Classification and identification may take advantage of tests exploring the glucose oxidation pathway.
Published Online:
Copyright 1989, International Association of Microbiological Societies
BascombS.,
LapageS. P.,
CurtisM. A.,
WilcoxW. R.1973; Identification of bacteria by computer: identification of reference strains. J. Gen. Microbiol. 77:291–315
BouvetO. M. M.,
GrimontP. A. D.1987; Diversity of the phosphoenolpyruvate/glucose phosphotransferase system in the Enterobacteriaceae. Ann. Inst. Pasteur (Paris) 138:3–13
BouvetO. M. M.,
GrimontP. A. D.1988; Extracellular oxidation of p-glucose by some members of the Enterobacteriaceae. Ann. Inst. Pasteur (Paris) 139:59–77
BrennerD. J.,
FanningG. R.,
KnudsonJ. K. Leete,
SteigerwaltA. G.,
KricevskyM. I.1984; Attempts to classify herbicola group-Enterobacter agglomerons strains by deoxyribonucleic acid hybridization and phenotypic tests. Int. J. Syst. Bacteriol. 34:45–55
GosselF.,
SwingsJ.,
De LeyJ.1980; A rapid, simple, and simultaneous detection of 2-keto-, 5-keto- and 2,5-diketogluconic acids by thin layer chromatography in culture media of acetic acid bacteria. Zentralbl. Bakteriol. Parasitenkd. Infektionskr. Hyg. Abt. 1 Orig. Reihe C 1:178–181
HommesR. W. J.,
PostmaP. W.,
NeijsselO. M.,
TempestD. W.,
DokterP.,
DuineJ. A.1984; Evidence of a quinoprotein glucose dehydrogenase apoenzyme in several strains of Escherichia coli. FEMS Microbiol. Lett. 24:329–333
HughR,
LeifsonE.1953; The taxonomic significance of fermentative versus oxidative metabolism of carbohydrates by various gram-negative bacteria. J. Bacteriol. 66:24–26
MatsushitaK.,
ShinagawaE.,
InoueT.,
AdachiO.,
AmeyamaM.1986; Immunological evidence for two types of PQQ-dependent *sc*D*/sc*-glucose dehydrogenase in bacterial membranes and the location of the enzyme in Escherichia coli. FEMS Microbiol. Lett. 37:141–144
MeadowN. D.,
RevueltaR.,
ChenV. N.,
ColwellR. R.,
RosemanS.1987; Phosphoenolpyruvatetglucose phosphotransferase system in species of Vibrio, a widely distributed marine bacterial genus. J. Bacteriol. 169:4893–4900
NeijsselO. M,
TempestD. W.,
PostmaP. W.,
DuineJ. A.,
JznJ. Frank.
1983; Glucose metabolism by K+-limited Klebsiella aerogenes: evidence for the involvement of a quinoprotein glucose dehydrogenase. FEMS Microbiol. Lett. 20:35–39
RomanoA. H.,
TrifoneJ. D.,
BrustolonM.1979; Distribution of the phosphoenolpyruvate:glucose phosphotransferase system in fermentative bacteria. J. Bacteriol. 139:93–97
SonoyamaT.,
KageyamaB.,
YagiS.1987; Distribution of microorganisms capable of reducing 2,5-diketo*sc*-D*/sc*-gluconate to 2-keto*sc*-L*/sc*-gulonate. Agric. Biol. Chem 51:2003–2004