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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 39, Issue 1

Genotypic Relationships and Taxonomic Localization of Unclassified and Strains by Deoxyribonucleic Acid:Ribosomal Ribonucleic Acid Hybridizations Free

Abstract

Abstract

The deoxyribonucleic acid (DNA):ribosomal ribonucleic acid (rRNA) hybridization technique was used to reveal the relationships and taxonomic positions of an additional 83 strains belonging to 43 saprophytic or pathogenic species and 29 named and unnamed like strains. The DNA:rRNA hybrids were characterized by the following two parameters: (i) the temperature at which one-half of the hybrid was eluted and (ii) the percentage of rRNA binding (the amount of rRNA bound per 100 pg of filter-fixed DNA). We also used, for a limited number of strains, numerical analysis of carbon assimilation tests to delineate the finer taxonomic relationships of organisms. Of the 83 strains examined, 78 could be definitely assigned either to an rRNA branch or to an rRNA superfamily within the Only 25 of our strains belong in the genus sensu stricto (our rRNA branch). In general, about two-thirds of the named species have been misclassified and are distributed over at least seven genera all through the These organisms need to be reclassified and generically renamed according to their phylogenetic relationships. However, more detailed phenotypic and genotypic studies are necessary before definite nomenclatural proposals can be made. A comprehensive list of the phylogenetic affiliations of the species is included.

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Copyright 1989, International Association of Microbiological Societies
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1989-01-01
2024-04-20
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Genotypic Relationships and Taxonomic Localization of Unclassified Pseudomonas and Pseudomonas-Like Strains by Deoxyribonucleic Acid:Ribosomal Ribonucleic Acid Hybridizations
Int J Syst Evol Microbiol 39, 35 (1989); https://doi.org/10.1099/00207713-39-1-35
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