Representative strains of the test tube brush bacterium , previously recovered from subgingival dental plaque of chronic adult periodontitis patients, were examined by determining their serological reactivities, protein electrophoretic patterns, deoxyribonucleic acid homologies, and bone resorptive activities in vitro. Our results indicate the existence of a third subspecies, subsp. subsp. nov., in addition to the two previously described subspecies, subsp. and subsp. . The third subspecies is phosphatase negative and asaccharolytic and does not stimulate bone resorption in vitro. The type strain of subsp. is strain ATCC 43716.


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