RT Journal Article SR Electronic(1) A1 Husson, M. O. A1 Trinel, P. A. A1 Mielcarek, C. A1 Gavini, F. A1 Caron, C. A1 Izard, D. A1 Leclerc, H.YR 1988 T1 Specificity of a Monoclonal Antibody for Alkaline Phosphatase in Escherichia coli and Shigella Species JF International Journal of Systematic and Evolutionary Microbiology, VO 38 IS 2 SP 201 OP 206 DO https://doi.org/10.1099/00207713-38-2-201 PB Microbiology Society, SN 1466-5034, AB The specificity of monoclonal antibody 2E5 for the alkaline phosphatase of Escherichia coli was studied against the alkaline phosphatases of 251 other bacterial strains. The organisms used included members of the six species of the genus Escherichia (E. coli, E. fergusonii, E. hermannii, E. blattae, E. vulneris, E. adecarboxylata), 41 species representing the family Enterobacteriaceae, and, in addition, Pseudomonas aeruginosa, Aeromonas spp., Plesiomonas shigelloides, Acinetobacter calcoaceticus, and Vibrio cholerae non-01. Three methods were used. An enzyme-linked immunosorbent assay was performed against 2IU of alkaline phosphatase per ml; immunofluorescence against bacterial cells and Western blotting against periplasmic proteins were also used. All of our experiments demonstrated the high specificity of monoclonal antibody 2E5. This antibody recognized only E. coli (118 strains tested) and the four species of the genus Shigella (S. sonnei, S. flexneri, S. boydii, S. dysenteriae; 12 strains tested)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/00207713-38-2-201