The specificity of monoclonal antibody 2E5 for the alkaline phosphatase of was studied against the alkaline phosphatases of 251 other bacterial strains. The organisms used included members of the six species of the genus (), 41 species representing the family , and, in addition, spp., , and non-01. Three methods were used. An enzyme-linked immunosorbent assay was performed against 2IU of alkaline phosphatase per ml; immunofluorescence against bacterial cells and Western blotting against periplasmic proteins were also used. All of our experiments demonstrated the high specificity of monoclonal antibody 2E5. This antibody recognized only (118 strains tested) and the four species of the genus ; 12 strains tested).


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