Eleven sterol-nonrequiring mollicute strains isolated from gut contents of representatives of nine insect genera and two strains isolated from flower surfaces ( sp.) were found to be serologically similar to an insect-derived acholeplasma (strain TAC [T = type strain]) described briefly in an earlier report. Strain TAC, isolated from gut fluids of a tabanid fly () and shown in earlier studies to belong to the class , lacked a sterol requirement for growth and catabolized glucose, but did not hydrolyze arbutin, arginine, or urea. Most strains of the strain TAC cluster grew on serum-free medium alone or on serum-free medium supplemented with a 0.04% Tween 80 fatty acid mixture. The strains grew over a temperature range of 23 to 32°C (optimum, 30°C) but did not grow at 37°C. The guanine-plus-cytosine content of the deoxyribonucleic acid was 30 mol%. Strain TAC was serologically unrelated to the ten previously-established species in the genus and to six other unclassified sterol-nonrequiring strains isolated from plant or insect hosts. The data suggest that this group of new acholeplasmas is widespread in insects and may be disseminated among various plant surfaces during feeding excursions. Strain TAC (= ATCC 43706) is proposed as the type strain of sp. nov.


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