Sodium deoxycholate gel electrophoresis was used to analyze the lipopolysaccharides of a variety of species and strains of purple nonsulfur bacteria. The electrophoretic migration patterns found for various lipopolysaccharides indicated their S or R characters, differences in their O-chain lengths, the presence or absence of unsubstituted core stubs, and the approximate sizes of the repeating units. The gel patterns were similar for closely related species (e.g., and or and ). Species-specific migration patterns were noted for and . Identical electrophoretic mobilities were observed for different chemo-/serotypes within the same species (e.g., , and ). A good correlation between chemo-serotypes and migration patterns was observed. Thus, gel electrophoresis of lipopolysaccharides proved to be useful for chemotaxonomic investigations.


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