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Volume 38, Issue 1

Characterization of gen. nov., sp. nov., a Stem-Nodulating Nitrogen-Fixing Bacterium Isolated from Free

Abstract

Twenty stem- and root-nodulating bacterial strains isolated from stem nodules of were compared by numerical analysis of 221 phenotypic features with nine strains which effectively nodulate only the roots of this plant and with representative strains from the genera and . Representative organisms from the different clusters were investigated further, together with possibly related organisms, by performing comparative gel electrophoresis of whole-cell proteins and by performing deoxyribonucleic acid (DNA)-DNA and DNA-ribosomal ribonucleic acid (rRNA) hybridizations. H-labeled rRNA was prepared from stem- and root-nodulating bacterial strain ORS 571 (T = type strain); [C]rRNA from NZP 5549 was also used. The following conclusions were drawn: (i) the root-nodulating bacterial strains are genuine rhizobia; (ii) the stem- and root-nodulating strains are quite different from and , and thus they constitute a separate rRNA subbranch on the rRNA branch in rRNA superfamily IV; and (iii) the closest relative of these organisms is , but they are phenotypically and genotypically sufficiently different from the latter genus to deserve a separate generic rank. Because the feature of free-living nitrogen fixation is quite discriminative, a new genus, , is proposed, with one species, . The type strain is strain ORS 571 (= LMG 6465).

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Copyright © 1988 International Union of Microbiological Societies
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1988-01-01
2024-04-25
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References

  1. Claus D., Schaab-Engels C.ed 1977 Catalogue of strains. Deutsche Sammlung von Mikroorganismen,, 2nd. Gesellschaft für Strahlen- und Umweltforschung mbH; Munich:
     [Google Scholar]
  2. De Ley J. 1970; Reexamination of the association between melting point, buoyant density, and chemical base composition of deoxyribonucleic acid. J. Bacteriol. 101:738–754
     [Google Scholar]
  3. De Ley J., Tytgat R. 1970; Evaluation of membrane filter methods for DNA:DNA hybridizations. Antonie van Leeuwenhoek J. Microbiol. Serol. 36:193–207
     [Google Scholar]
  4. De Ley J., Van Muylem J. 1963; Some applications of deoxyribonucleic acid base composition in bacterial taxonomy. Antonie van Leeuwenhoek J. Microbiol. Serol. 29:344–358
     [Google Scholar]
  5. De Smedt J., De Ley J. 1977; Intra- and intergeneric similarities of Agrobacterium ribosomal ribonucleic acid cistrons. Int. J. Syst. Bacteriol. 27:222–240
     [Google Scholar]
  6. De Vos P., Goor M., Gillis M., De Ley J. 1985; Ribosomal ribonucleic acid cistron similarities of phytopathogenic Pseudomonas species. Int. J. Syst. Bacteriol. 35:169–184
     [Google Scholar]
  7. Doetsch R. N. 1981 Determinative methods of light microscopy. 21–33 Gerhardt P., Murray R. G. E., Costilow R. N., Nester E. W., Wood W. A., Krieg N. R., Phillips G. B.ed Manual of methods for general bacteriology American Society for Microbiology; Washington, D.C:
     [Google Scholar]
  8. Dreyfus B., Alazard D., Dommergues Y. R. 1984 Stemnodulating rhizobia. 161–169 Klug M. C., Reddy C. E.ed Current perspectives in microbial ecology American Society for Microbiology; Washington, D.C:
     [Google Scholar]
  9. Dreyfus B., Dommergues Y. R. 1981; Nitrogen-flxing nodules induced by Rhizobium on the stem of the tropical legume Sesbania rostrata. FEMS Microbiol. Lett. 10:313–317
     [Google Scholar]
  10. Dreyfus B. L., Elmerich C., Dommergues Y. R. 1983; Free-living Rhizobium strains able to grow on N2 as the sole nitrogen source. Appl. Environ. Microbiol. 45:711–713
     [Google Scholar]
  11. Garcia J. L., Roussos S., Bensoussan M., Bianchi A., Mandel M. 1982; Taxonomie numérique de Bacillus thermophiles isolés de sols de rhizière de l’Ouest. Ann. Inst. Pasteur Paris 133A:471–488
     [Google Scholar]
  12. Garcia J. L., Roussos S., Gauthier D., Rinaudo G., Mandel M. 1983; Etude taxonomique de bactéries azotofixatrices libres isolées de l’endorhizosphère du riz. Ann. Inst. Pasteur Paris 134B:329–346
     [Google Scholar]
  13. Gebhardt C., Turner G. L., Gibson A. H., Dreyfus B. L., Bergersen F. J. 1984; Nitrogen-fixing growth in continuous culture of a strain of Rhizobium sp. isolated from stem-nodules on Sesbania rostrata. J. Gen. Microbiol. 130:843–846
     [Google Scholar]
  14. Gerhardt P., Murray R. G. E., Costilow R. N., Nester E. W., Wood W. A., Krieg N. R., Phillips G. B.ed 1981 Manual of methods for general bacteriology. American Society for Microbiology; Washington, D.C:
     [Google Scholar]
  15. Gillis M., De Ley J. 1980; Intra- and intergeneric similarities of the ribosomal ribonucleic acid cistrons of Acetobacter and Gluconobacter. Int. J. Syst. Bacteriol. 30:7–27
     [Google Scholar]
  16. Jarvis B. D. W., Gillis M., De Ley J. 1986; Intra- and intergeneric similarities between ribosomal ribonucleic acid cistrons of Rhizobium and Bradyrhizobium species and some related bacteria. Int. J. Syst. Bacteriol. 36:129–138
     [Google Scholar]
  17. Jordan D. C. 1984 Family III. Rhizobiaceae Conn 1938. 234–244 Krieg N. R., Holt J. G.ed Bergey’s manual of systematic bacteriology 1 The William & Wilkins Co.; Baltimore.:
     [Google Scholar]
  18. Juthner R. R., Lafferty R. M., Knackmuss H. J. 1975; A simple method for the determination of poly-β-hydroxybutyric acid in microbial biomass. Eur. J. Appl. Microbiol. 1:233–237
     [Google Scholar]
  19. Kersters K. 1985 Numerical methods in the classification of bacteria by protein electrophoresis. 337–368 Goodfellow M., Jones D., Priest F. G.ed Computer-assisted bacterial systematics Academic Press, Inc. (London), Ltd.; London:
     [Google Scholar]
  20. Kiredjian M., Holmes B., Kersters K., Guilvout I., De Ley J. 1986; Alcaligenes piechaudii, a new species from human clinical specimens and the environment. Int. J. Syst. Bacteriol. 36:282–287
     [Google Scholar]
  21. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227:680–685
     [Google Scholar]
  22. Lovelace T. E., Colwell R. R. 1968; A multipoint inoculator for petri dishes. Appl. Microbiol. 18:944–945
     [Google Scholar]
  23. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J. Mol. Biol. 5:109–118
     [Google Scholar]
  24. Nishimura Y., Yamanaka S., Iizuka H. I. 1983; Ubiquinone, fatty acid, polar lipid and DNA base composition in Pseudomonas radiora. J. Gen. Appl. Microbiol. 29:421–427
     [Google Scholar]
  25. Reinhold B., Hurek T., Fendrik I., Pot B., Gillis M., Kersters K., Thielemans S., De Ley J. 1987; Azospirillum halopraeferens sp. nov., a nitrogen-fixing organism associated with roots of Kallar grass (Leptochloa fusca (L.) Kunth). Int. J. Syst. Bacteriol. 37:43–51
     [Google Scholar]
  26. Rhodes M. E. 1958; The cytology of Pseudomonas spp. as revealed by a silver-plating staining method. J. Gen. Microbiol. 18:639–648
     [Google Scholar]
  27. Richards G. M. 1974; Modification of the diphenylamine reaction giving increased sensitivity and simplicity in the estimation of DNA. Anal. Biochem. 57:369–376
     [Google Scholar]
  28. Van Landschoot A., De Ley J. 1983; Intra- and intergeneric similarities of the rRNA cistrons of Alteromonas, Marinomonas(gen. nov.) and some other gram-negative bacteria. J. Gen. Microbiol. 129:3057–3074
     [Google Scholar]
  29. Vincent J. M. 1970 A manual for the practical study of root-nodule bacteria. 73–97 International Biological Programme Handbook Blackwell Scientific Publications, Ltd.; Oxford:
     [Google Scholar]
  30. Wiegel J. K. W., Schlegel H. G. 1984 Genus Xanthobacter Wiegel, Wilke, Baumgarten, Opitz and Schlegel. 325–333 Krieg N. R., Holt J. G.ed Bergey’s manual of systematic bacteriology 1 The Williams & Wilkins Co.; Baltimore.:
     [Google Scholar]
  31. Wiegel J., Wilke D., Baumgarten J., Opitz R., Schlegel H. G. 1978; Transfer of the nitrogen-fixing hydrogen bacterium Corynebacterium autotrophicum Baumgarten et al. to Xanthobacter gen. nov. Int. J. Syst. Bacteriol. 28:573–581
     [Google Scholar]
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Characterization of Azorhizobium caulinodans gen. nov., sp. nov., a Stem-Nodulating Nitrogen-Fixing Bacterium Isolated from Sesbania rostrata
Int J Syst Evol Microbiol 38, 89 (1988); https://doi.org/10.1099/00207713-38-1-89
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