1887

Abstract

The type strain ATCC 27155 was examined for deoxyribonucleic acid (DNA) relatedness to 54 strains of the “ complex,” to 23 strains of other and species, and to 50 reference strains of 49 different species in other genera of the family . The DNA-DNA hybridization values (at the optimal renaturation temperature; nitrocellulose filter method) showed that 24 strains were highly related to ATCC 27155 (62 to 97% DNA relatedness) and formed a genotypic group provisionally called DNA hybridization group 27155. These strains were received as (including strains of DNA hybridization groups V and XIII [D. J. Brenner, G. R. Fanning, J. K. Leete Knutson, A. G. Steigerwalt, and M. I. Krichevsky, Int. J. Syst. Bacteriol. 34:45-55, 1984]); as , including the type strain NCPPB 2971; as , including the type strain NCPPB 2519; and as yellow-pigmented strains. Numerical analysis of the protein electropherograms of these strains revealed the existence of seven protein electrophoretic groups, each showing a characteristic protein pattern. These seven groups separated at an infraspecific level and allowed assignment of 37 additional strains, received as , and , to DNA hybridization group 27155. The resulting group hybridized below 55% DNA relatedness with all remaining phenotypic or genotypic groups of the “ complex” and below 57% DNA relatedness with other and species. DNA binding with 49 other species of the family was less than 38%. Since DNA hybridization group 27155 contains the type strains of , and , these species names are subjective synonyms, and the specific epithet has priority. Further genotypic studies with several closely related genera are required for final placement of this species in a genus. The description of , and is emended.

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1988-01-01
2024-06-25
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