RT Journal Article SR Electronic(1) A1 Willems, A. A1 Gillis, M. A1 Kersters, K. A1 Van Den Broecke, L. A1 De Ley, J.YR 1987 T1 Transfer of Xanthomonas ampelina Panagopoulos 1969 to a New Genus, Xylophilus gen. nov., as Xylophilus ampelinus (Panagopoulos 1969) comb. nov. JF International Journal of Systematic and Evolutionary Microbiology, VO 37 IS 4 SP 422 OP 430 DO https://doi.org/10.1099/00207713-37-4-422 PB Microbiology Society, SN 1466-5034, AB Thirty-four strains of Xanthomonas ampelina, the causal agent of bacterial necrosis of grape vines, were examined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of their cellular proteins and by numerical analysis of 106 enzymatic features (API systems). These organisms formed a very homogeneous taxon. Generic and suprageneric relationships were determined by hybridizations between 23S 14C-labeled ribosomal ribonucleic acid from Xanthomonas ampelina NCPPB 2217T (T = type strain) and deoxyribonucleic acids from eight Xanthomonas ampelina strains, Xanthomonas campestris NCPPB 528T, and the type strains of 16 possibly related species. Xanthomonas ampelina was found to be a totally separate subbranch in ribosomal ribonucleic acid superfamily III, without any relatives at the generic level. It is not related to the genus Xanthomonas. Genetically its closest relatives are, among others, Pseudomonas acidovorans, Alcaligenes paradoxus, and Comamonas terrigena. We propose the transfer of Xanthomonas ampelina to a new genus, Xylophilus. The only species and thus the type species is Xylophilus ampelinus. The type strain is NCPPB 2217., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/00207713-37-4-422