1887

Abstract

Cloned deoxyribonucleic acid (DNA) fragments, derived from a cosmid library of a Florida isolate of pv. 3041 were used to detect restriction fragment-length polymorphism among 93 strains of , which make up 26 pathovars. DNA clones were radiolabeled and hybridized against Southern blot transfers of genomic DNAs of different strains of digested with restriction endonucleases. Autoradiographs for DNA clones probed against genomic DNA revealed hybridization profiles which appeared to be highly conserved and unique for each pathovar tested. As a species, the population structure of appeared basically clonal. Variability appeared to depend on the DNA probe used; some probes represented DNA loci which were highly variable, and some represented DNA loci which were highly conserved at the species level. By using more than one DNA probe, or by digesting the genomic DNAs with different restriction endonucleases, we were able to differentiate all of the strains of described as belonging to a given pathovar. Differences among the pathovars were also confirmed by pathogenicity experiments on plants. Some phytopathogenic strains isolated from plants not previously described as susceptible to could be grouped as being related to previously described pathovars.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-37-3-214
1987-07-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/37/3/ijsem-37-3-214.html?itemId=/content/journal/ijsem/10.1099/00207713-37-3-214&mimeType=html&fmt=ahah

References

  1. Alvarez A. M., Benedict A. A., Mizumoto C. Y. 1985; Identification of xanthomonads and grouping of strains of Xanthomonas campestris pv. campestris with monoclonal antibodies. Phytopathology 75:722–728
    [Google Scholar]
  2. Barker D., Schafer M., White R. 1984; Restriction sites containing GpG show a higher frequency of polymorphism in human DNA. Cell 36:131–138
    [Google Scholar]
  3. Bradbury J. F. 1984 Xanthomonas Dowson 1939. 199–210 Krieg N. R., Holt J. G.ed Bergey’s manual of systematic bacteriology 1 The Williams and Wilkins Co.; Baltimore:
    [Google Scholar]
  4. Bufton L., Bruns G. A. P., Magenis R. E., Tomar D., Shaw D., Brook D., Litt M. 1986; Four restriction fragment length polymorphisms revealed by probes from a single cosmid map to chromosome 19. Am. J. Human Genet. 38:447–460
    [Google Scholar]
  5. Close T. J., Zaitlin D., Kado C. I. 1984; Design and development of amplifiable broad-host-range cloning vectors: analysis of the vir region of Agrobacterium tumefaciens plasmid pTiC58. Plasmid 12:111–118
    [Google Scholar]
  6. Daniels M. J., Barber C. E., Turner P. C., Sawczyc M. K., Byrde R. J. W., Fielding A. H. 1985; Cloning of genes involved in pathogenicity of Xanthomonas campestris pv. campestris using the broad host range cosmid pLAFRl. EMBO J. 13:3323–3328
    [Google Scholar]
  7. Daub M. E., Hagedorn D. J. 1981; Epiphytic populations of Pseudomonas syringae on susceptible and resistant bean lines. Phytopathology 71:547–550
    [Google Scholar]
  8. De Vos P., Goor M., Gillis M., De Ley J. 1985; Ribosomal ribonucleic acid cistron similarities of phytopathogenic Pseudomonas species. Int. J. Syst. Bacteriol. 35:169–184
    [Google Scholar]
  9. Dye D. W. 1958; Host specificity of Xanthomonas. Nature 182:1813–1814
    [Google Scholar]
  10. Ercolani G. L. 1978; Pseudomonas savastanoi and other bacteria colonizing the surface of olive leaves in the field. J. Gen. Microbiol. 109:245–257
    [Google Scholar]
  11. Gabriel D. W., Burges A., Lazo G. R. 1986; Gene-for-gene interactions of five cloned avirulence genes from Xanthomonas campestris pv. malvacearum with specific resistance genes in cotton. Proc. Natl. Acad. Sci. USA 83:6415–6419
    [Google Scholar]
  12. Goto M., Starr M. P. 1972; Phage-host relationships of Xanthomonas citri compared with those of other Xanthomonas. Ann. Phytopathol. Soc. Jpn. 38:226–248
    [Google Scholar]
  13. Gottlieb P., Rudner R. 1985; Restriction site polymorphism of ribosomal ribonucleic acid gene sets in members of the genus Bacillus. Int. J. Syst. Bacteriol. 35:244–252
    [Google Scholar]
  14. Helentjaris T., King G., Slocum M., Siedenstang C., Wegman S. 1985; Restriction fragment polymorphisms as probes for plant diversity and their development as tools for applied plant breeding. Plant Mol. Biol. 5:109–118
    [Google Scholar]
  15. Kamper S. M., French W. J., deKloet S. R. 1985; Genetic relationships of some fastidious xylem-limited bacteria. Int. J. Syst. Bacteriol. 35:185–188
    [Google Scholar]
  16. Lazo G. R., Gabriel D. W. 1987; Conservation of plasmid DNA sequences and pathovar identification of strains of Xanthomonas campestris. Phytopathology 77:448–453
    [Google Scholar]
  17. Leyns F., De Cleene M., Swing J., De Ley J. 1984; The host range of the genus Xanthomonas. Bot. Rev. 50:308–356
    [Google Scholar]
  18. Li W.-H. 1986; Evolutionary change of restriction cleavage sites and phylogenetic inference. Genetics 113:187–213
    [Google Scholar]
  19. Liew K. W., Alvarez A. M. 1981; Phage typing and lysotype distribution of Xanthomonas campestris. Phytopathology 71:274–275
    [Google Scholar]
  20. Litt M., White R. L. 1985; A highly polymorphic locus in human DNA revealed by cosmid-derived probes. Proc. Natl. Acad. Sci. USA 82:6206–6210
    [Google Scholar]
  21. Maas J. L., Finney M. M., Civerolo E. L., Sasser M. 1985; Association of an unusual strain of Xanthomonas campestris with apple. Phytopathology 75:438–445
    [Google Scholar]
  22. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory; Cold Spring Harbor, N.Y:
    [Google Scholar]
  23. McClean P. E., Hanson M. R. 1986; Mitochondrial DNA sequence divergence among Lycopersicon and related Solanum species. Genetics 112:649–667
    [Google Scholar]
  24. Minsavage G. V., Schaad N. W. 1983; Characterization of membrane proteins of Xanthomonas campestris pv. campestris. Phytopathology 73:747–755
    [Google Scholar]
  25. Mulrean E. N., Schroth M. M. 1982; Ecology of Xanthomonas campestris pv. juglandis on Persian (English) walnuts. Phytopathology 72:434–438
    [Google Scholar]
  26. Murata N., Starr M. P. 1973; A concept of the genus Xanthomonas and its species in the light of segmental homology of deoxyribonucleic acids. Phytopathol. Z. 77:285–323
    [Google Scholar]
  27. Nei M., Li W.-H. 1979; Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc. Natl. Acad. Sci. USA 76:5269–5273
    [Google Scholar]
  28. Schleifer K. H., Ludwig W., Kraus J., Festl H. 1985; Cloned ribosomal ribonucleic acid genes from Pseudomonas aeruginosa as probes for conserved deoxyribonucleic acid sequences. Int. J. Syst. Bacteriol. 35:231–236
    [Google Scholar]
  29. Schnathorst W. C. 1966; Unaltered specificity in several xanthomonads after repeated passage through Phaseolus vulgaris. Phytopathology 56:58–60
    [Google Scholar]
  30. Schroth M. N., Hildebrand D. C. 1983; Toward a sensible taxonomy of bacterial plant pathogens. Plant Dis. 67:128
    [Google Scholar]
  31. Silhavy T. J., Berman M. L., Enquist L. W. 1984 Experiments with gene fusions. Cold Spring Harbor Laboratory; Cold Spring Harbor, N.Y:
    [Google Scholar]
  32. Stall R. E., Loschke D. C., Jones J. B. 1986; Linkage of copper resistance and avirulence loci on a self-transmissible plasmid in Xanthomonas campestris pv. vesicatoria. Phytopathology 76:240–243
    [Google Scholar]
  33. Sytsma K. J., Gottlieb L. D. 1986; Chloroplast DNA evidence for the origin of the genus Heterogaura from a species of Clarkia (Onagraceae). Proc. Natl. Acad. Sci. USA 83:55545557
    [Google Scholar]
  34. Thaveechai N., Schaad N. W. 1984; Comparison of different immunogen preparations for serological identification of Xanthomonas campestris pv. campestris. Phytopathology 74:1065–1070
    [Google Scholar]
  35. Thaveechai N., Schaad N. W. 1986; Immunochemical characterization of a subspecies-specific antigenic determinant of a membrane protein extract of Xanthomonas campestris pv. campestris. Phytopathology 76:148–153
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-37-3-214
Loading
/content/journal/ijsem/10.1099/00207713-37-3-214
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error