1887

Abstract

Mycoplasma strain 163K was isolated from the gills of a tench () with red disease. The cells are elongated, ovoid or flask shaped, consisting of a thicker body and a more slender part ending in a hemispherical terminal structure, that is apparently stabilized by a cytoskeleton. They are able to attach to inert surfaces and living cells. The most exciting property of the organisms is their fast gliding motion, which occurs on glass and plastic surfaces and which is not interrupted by resting periods. Growth occurs between 17 and 30°C, with an optimum at 25°C, in modified Hayflick medium with horse or bovine serum and in medium with swine serum. Acid is produced from several carbohydrates, but arginine and urea are not catabolized. The organism reduces tetrazolium chloride, produces film and spots and hydrogen peroxide, and is able to hemolyze and to adsorb to erythrocytes of several animal species. Indirect immunofluorescence test, growth inhibition, and metabolism inhibition tests indicate that the mycoplasma represents a new species for which the name is proposed. Strain 163K (ATCC 43663) is the type strain.

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1987-07-01
2024-11-11
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References

  1. Albers A. C., Fletcher R. D. 1982; Simple method for quantitation of viable mycoplasmas. Appl. Environ. Microbiol. 43:958–960
    [Google Scholar]
  2. Allen T. C., Stevens J. O., Florance E. R., Hampton R. O. 1970; Ultrastructure of Mycoplasma gallisepticum isolate 1056. J. Ultrastruct. Res. 33:318–331
    [Google Scholar]
  3. Aluotto B. B., Wittier R. G., Williams C. O., Faber J. E. 1970; Standardized bactériologie techniques for the characterization of Mycoplasma species. Int. J. Syst. Bacteriol. 20:35–58
    [Google Scholar]
  4. Eiberfeld G., Biberfeld P. 1970; Ultrastructural features of Mycoplasma pneumoniae. J. Bacteriol. 102:855–861
    [Google Scholar]
  5. Eredt W. 1979 Motility. 141–155 Barile M. F., Razin S.ed The mycoplasmas I Academic Press, Inc.; New York:
    [Google Scholar]
  6. Entier M., Knight B. C. J. G. 1956; Growth curves of strains of pleuropneumonia-like organisms. J. Gen. Microbiol. 14:vi–vii
    [Google Scholar]
  7. Chanock R. M., Hayflick L., Barile M. F. 1962; Growth on artificial medium of an agent associated with atypical pneumo nia and its identification as a pleuropneumonia-like organism. Proc. Natl. Acad. Sei. USA 48:41–48
    [Google Scholar]
  8. Clyde W. A. 1964; Mycoplasma species identification based upon growth inhibition by specific antisera. J. Immunol 92:958–965
    [Google Scholar]
  9. Del Giudice R. A., Robillard F., Carski T. R. 1967; Immunofluorescence identification of mycoplasma on agar by use of incident illumination. J. Bacteriol. 93:1205–1209
    [Google Scholar]
  10. Del Giudice R. A., Tully J. G., Rose D. L., Cole R. M. 1985; Mycoplasma pirum sp. nov., a terminal structured mollicute from cell cultures. Int. J. Syst. Bacteriol. 35:285–291
    [Google Scholar]
  11. Flossdorf J. 1983; A rapid method for the determination of the base composition of bacterial DNA. J. Microbiol. Methods 1:305–311
    [Google Scholar]
  12. Freundt E. A., Andrews B. E., Ernp H., Kunze M., Black F. T. 1973; The sensitivity of Mycoplasmatales to sodium polyanethol-sulfonate and digitonin. Zentralbl. Bakteriol. Abt. 1 Orig. Reihe A 225:104–112
    [Google Scholar]
  13. Friis F. 1975; Some recommendations concerning primary isolation of Mycoplasma suipneumöniae and Mycoplasma flocculare. Nord. Vet. Med. 27:337–339
    [Google Scholar]
  14. Gourlay R. N., Wyld S. C., Leach R. H. 1977; Mycoplasma alvi, a new species from bovine intestinal and urogenital tracts. Int. J. Syst. Bacteriol. 27:89–96
    [Google Scholar]
  15. Gourlay R. N., Wyld S. C., Leach R. H. 1978; Mycoplasma sualvi, a new species from the intestinal and urogenital tracts of pigs. Int. J. Syst. Bacteriol. 28:289–292
    [Google Scholar]
  16. Hümmeler K., Tomassini N., Hayflick L. 1965; Ultrastructure of mycoplasma (Negroni) isolated from human leukemia. J. Bacteriol. 90:517–523
    [Google Scholar]
  17. Kirchhoff H., Rosengarten R. 1984; Isolation of a motile mycoplasma from fish. J. Gen. Microbiol. 130:2439–2445
    [Google Scholar]
  18. Kirchhoff H., Rosengarten R., Lotz W., Fischer M., Lopatta D. 1984; Flask-shaped mycoplasmas properties and pathogenicity for man and animals. Isr. J. Med. Sei. 20:848–853
    [Google Scholar]
  19. Luft J. H. 1961; Improvements in epoxy resin embedding methods. J. Biophys. Biochem. Cytol. 9:409–414
    [Google Scholar]
  20. Morton H. E., Roberts R. J. 1966; Productiort of antimycoplasma (PPLO) antibodies in rabbits. Proc. Soc. Exp. Biol. Med. 125:538–542
    [Google Scholar]
  21. Reynolds E. S. 1963; The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J. Cell. Biol 17:208–212
    [Google Scholar]
  22. Sobeslavsky O., Prescott B., Chanock R. M. 1968; Adsorption of Mycoplasma pneumoniae to neuraminic acid receptors of various cells and possible role in virulence. J. Bacteriol 96:695–705
    [Google Scholar]
  23. Subcommittee on the Taxonomy of Mollicutes 1979; Proposal of minimal standards for description of new species of the class Mollicutes. Int. J. Syst. Bacteriol. 29:172–180
    [Google Scholar]
  24. Taylor-Robinson D., Bredt W. 1983; Motility of Mycoplasma strain G37. Yale J. Biol. Med. 56:910–911
    [Google Scholar]
  25. Taylor-RobinSon D., Purcell H., Wong D. C., Chanock R. M. 1966; A colour test for the measurement of antibody to certain Mycoplasma species based upon the inhibition of acid production. J. Hyg. 64:91–104
    [Google Scholar]
  26. Tully J. G. 1983 Test for digitonin sensitivity and sterol requirements. 357–362 Razin S., Tully J. G.ed Methods in mycoplasmology 1 Academic Press, Inc.; New York:
    [Google Scholar]
  27. Tully J. G., Taylor-Robinson D., Rose D. L., Cole R. M., Bove J. M. 1983; Mycoplasma genitalium, a new species from the human urogenital tract. Int. J. Syst. Bacteriol. 33:387–396
    [Google Scholar]
  28. Watson M. L. 1958; Staining of tissue sections for electron microscopy with heavy metals. J. Biophys. Biochim. Cytol 4:475–478
    [Google Scholar]
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