1887

Abstract

Five strains of a rapidly growing, orange, scotochromogenic species were isolated from a marine sponge. Although they displayed similarities to and , they proved to belong to a proposed new species, sp. nov. (ATCC 35087). We distinguished the type strain of from that of by its pattern of carbon sources used, acid production from carbon sources, and amidases, by its ability to tolerate 5% sodium chloride, its failure to use benzamide as sole nitrogen source, and its use of -serine as sole carbon and nitrogen source. We distinguished the type strain of from that of by its pattern of carbon sources used, acid production from carbon sources, and amidases, by its ability to tolerate 5% sodium chloride, its failure to grow at 42°C, its strong pigmentation, its failure to reduce nitrate, its failure to tolerate 0.0250% hydroxylamine hydrochloride, its ability to use -serine as dual carbon and nitrogen source, and its failure to use acetamide as dual carbon and nitrogen source. The pattern of mycolic acid derivatives produced by acid methanolysis of whole organisms was that of the group typified by and which contains α-mycolates, ketomycolates, and wax ester derivatives.

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1987-07-01
2024-10-14
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References

  1. Difco Laboratories, Inc. 1984 Difco Manual, 10th.567–569 Difco Laboratories, Inc.; Detroit, Mich:
    [Google Scholar]
  2. Hecht S. T., Causey W. A. 1976; Rapid method for the detection and identification of mycolic acids in aerobic actinomycètes and related bacteria. J. Clin. Microbiol 4:284–287
    [Google Scholar]
  3. Juhlin I. 1967; Contribution of the classification of mycobacteria and nocardias. Acta Pathol. Microbiol. Scand 70:Suppl189
    [Google Scholar]
  4. Kilburn J. O., O’Donnell K. F., Silcox V. A., David H. L. 1973; Preparation of a stable mycobacterial Tween hydrolysis substrate. Appl. Microbiol. 26:826
    [Google Scholar]
  5. McIntosh K. S., Moshier S. E. 1979; Carotenogenesis in a marine sponge, Halichondria bowerbanki. Proc. Nebr. Acad. Sci.15
    [Google Scholar]
  6. Minnikin D. E. 1982 Lipids: complex lipids, their chemistry, biosynthesis and roles. 95–184 Ratledge C., Stanford J.ed The biology of the mycobacteria I Academic Press, Inc.; New York:
    [Google Scholar]
  7. Minnikin D. E., Hutchinson I. G., Caldicott A. B. 1980; Thin-layer chromatography of methanolysates of mycolic acidcontaining bacteria. J. Chromatogr. 188:221–233
    [Google Scholar]
  8. Minnikin D. E., Minnikin S. M., Parlett J. H., Goodfellow M. 1985; Mycolic acid patterns of some rapidly-growing species of Mycobacterium. Zentralbl. Bakteriol. Parasitenkd. Infektionskr. Hyg. Abt. 1 Reihe A 259:446–460
    [Google Scholar]
  9. Morse W. C., Blair E. B., Weiser O. L., Sproat E. F. 1968 Mycobacteriology laboratory methods. U. S. Army Medical Research and Nutrition Laboratory Fitzsimons General Hospital; Denver, Colo:
    [Google Scholar]
  10. Moshier S. E. 1983; Isolation, purification, and identification of the aromatic carotenoid isorenieratene in the marine demosponge Halichondria bowerbanki. 13 Proc. Nebr. Acad. Sci. p. 15
    [Google Scholar]
  11. Runyon E. H., Wayne L. G., Kubica G. P. 1974 Family IL Mycobacteriaceae Chester 1897. 63681–701 Buchanan R. E., Gibbons N. E.ed Bergey’s manual of determinative bacteriology, 8th. The Williams and Wilkins Co.; Baltimore:
    [Google Scholar]
  12. Saito H., Gordon R. E., Juhlin I., Kappler W., Kwapinski J. B. G., McDurmont C., Pattyn S. R., Runyon E. H., Stanford J. L., Tarnok I., Tasaka H., Tsukamura M., Weiszfeiler J. 1977; Comparative numerical analysis of rapidly growing mycobacteria. The second report. Int. J. Syst. Bacteriol. 27:75–85
    [Google Scholar]
  13. Smithwick R. W. 1976 Laboratory manual for acid-fast microscopy. Center for Disease Control; Atlanta:
    [Google Scholar]
  14. Tarshis M. S. 1964; A PAS medium as an aid in differentiating human and bovine tubercle bacilli and Mycobacterium fortuitum from other mycobacteria. Tubercle 45:267–271
    [Google Scholar]
  15. Tsukamura M. 1961; Certain saprophytic mycobacteria front soil sources forming a red color product from PAS. Am. Rev. Respir. Dis. 84:916
    [Google Scholar]
  16. Tsukamura M. 1961; Formation of a red color product from PAS by certain Mycobacteria. Jpn. Tuberc. 9:70
    [Google Scholar]
  17. Tsukamura M. 1965; Salicylate degradation test for differentiation of Mycobacterium fortuitum from other Mycobacteria. J. Gen. Microbiol. 41:309–315
    [Google Scholar]
  18. Tsukamura M. 1965; Differentiation of mycobacteria by picric acid tolerance. Am. Rev. Respir. Dis. 92:491–492
    [Google Scholar]
  19. Tsukamura M. 1965; Differentiation of mycobacteria by susceptibility to hydroxylamine and 8-azaguanine. J. Bacteriol. 90:556–557
    [Google Scholar]
  20. Tsukamura M. 1966; Adansonian classification of mycobacteria. J. Gen. Microbiol. 45:253–273
    [Google Scholar]
  21. Tsukamura M. 1966; Mycobacterium parafortuitum, a new species. J. Gen. Microbiol. 42:7–12
    [Google Scholar]
  22. Tsukamura M. 1967; Identification of mycobacteria. Tubercle 48:311–339
    [Google Scholar]
  23. Tsukaihura M. 1968; Relationship between growth rate of Mycobacteria and their ability to utilize organic acids as the sole source of carbon. Jpn. J. Microbiol. 12:534–536
    [Google Scholar]
  24. Tsukamura M., Mizuno S. 1968; “Hypothetical mean organisms” of Mycobacteria. Jpn. J. Microbiol. 12:371–384
    [Google Scholar]
  25. Tsukamura M., Mizuno S., Tsukamura S. 1981; Numerical Analysis of Rapidly Growing, Scotochromogenic Mycobacteria, Including Mycobacterium obuense sp. nov., nom. rev., Mycobacterium rhodesiae sp. nov., nom. rev., Mycobacterium aichiense sp. nov., nom. rev., Mycobacterium chubuense sp. nov., nom. rev., and Mycobacterium tokaiense sp. nov., nom. rev. Int. J. Syst. Bacteriol. 31:263–275
    [Google Scholar]
  26. Tsukamura M., Mizuho S., Tsukamura S., Tsukamura J. 1979; Comprehensive numerical classification of 369 strains of Mycobacterium, Rhodococcus, and Nocardia. Int. J. Syst. Bacteriol. 29:110–129
    [Google Scholar]
  27. Tsukamura M., Yario I., Imaeda T. 1986; Mycobacterium moriokaense sp. nov., a rapidly growing, nonphotochromogenic Mycobacterium. Int. J. Syst. Bacteriol. 36:333–338
    [Google Scholar]
  28. Vestal A. L. 1975 Procedures for the isolation and identification of mycobacteria. Cehter for Disease Control; Atlanta:
    [Google Scholar]
  29. Wàyne L. G., Doubek J. R., Russell R. L. 1964; Classification and identification of mycobacteria. I. Tests employing Tween 80 as substrate. Am. Rev. Respir. Dis. 90:588–597
    [Google Scholar]
  30. Wayne L. G., Doubek J. R. 1968; Diagnostic key to Mycobacteria encountered in clinical laboratories. Appl. Microbiol. 16:925–931
    [Google Scholar]
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