1887

Abstract

Immunodiffusion and an enzyme-linked immunosorbent assay (ELISA) were used to reveal serological relationships between coryneform bacteria responsible for annual ryegrass toxicity (ARGT) and other plant-pathogenic corynebacteria, particularly those associated with similar diseases of grasses, , and . Antisera were raised in rabbits against one strain each of , and the ARGT bacterium. The antisera were tested against 26 strains belonging to that group of species and one strain each of eight other species of plant-pathogenic corynebacteria. Immunodiffusion with antiserum against the ARGT bacterium did not show cross-reactions with any of the other species tested, whereas, antisera against , and all showed some heterologous cross-reactions. The ELISA revealed cross-reactions undetected by immunodiffusion and allowed the organisms to be grouped by hierarchical cluster analysis. The results indicate that coryneform bacteria pathogenic to and responsible for its toxicity to livestock are serologically distinct from the other plant-pathogenic corynebacteria causing similar diseases of grasses. Amino acid analysis of the ARGT bacterium cell wall revealed the presence of 2,4-diaminobutyric acid, indicating that this bacterium should be classified in the genus .

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1987-04-01
2024-12-08
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References

  1. Alvey N. G., Banfield S. F., Baxter R. K., Gower J. C., Krzanowski W. J., Lane P. W., Leech P. W., Nelder J. A., Payne R. W., Phelps K. M., Rogers C. E., Ross G. J. S., Simpson H. R., Todd A. D., Tunnicliffe-Wilson G., Wedderburn R. W. M., White R. P., Wilkinson G. M. 1983 GENSTAT, a general statistical program. Rothamsted Experimental Station. The Numerical Algorithms Group Ltd.; Oxford:
    [Google Scholar]
  2. Bird A. F., Riddle D. L. 1984; Effect of Corynebacterium rathayi on movement of Anguina agrostis larvae. Int. J. Parasitol. 14:503–511
    [Google Scholar]
  3. Bird A. F., Stynes B. A. 1977; The morphology of a Corynebacterium sp. parasitic on annual rye grass. Phytopathology 67:828–830
    [Google Scholar]
  4. Bird A. F., Stynes B. A. 1981; The life cycle of Anguina agrostis’. embryogenesis. Int. J. Parasitol. 11:23–33
    [Google Scholar]
  5. Bird A. F., Stynes B. A. 1981; The life cycle of Anguina agrostis-. post-embryonic growth of the second stage larvae. Int. J. Parasitol. 11:243–250
    [Google Scholar]
  6. Bird A. F., Stynes B. A. 1981; The life cycle of Anguina agrostis-. development in host plant. Int. J. Parasitol. 11:431440
    [Google Scholar]
  7. Bird A. F., Stynes B. A., Thomson W. W. 1980; A comparison of nematode and bacteria-colonized galls induced by Anguina agrostis in Lolium rigidum. Phytopathology 70:1104–1109
    [Google Scholar]
  8. Bradbury J. F. 1973 Corynebacterium rathayi. Descriptions of pathogenic fungi and bacteria, no. 376. Commonwealth Agricultural Bureaux; London:
    [Google Scholar]
  9. Bradbury J. F. 1973 Corynebacterium tritici. Descriptions of pathogenic fungi and bacteria, no. 377. Commonwealth Agricultural Bureaux; London:
    [Google Scholar]
  10. Carlson R. R., Vidaver A. K. 1982; Taxonomy of Corynebacterium plant pathogens, including a new pathogen of wheat, based on polyacrylamide gel electrophoresis of cellular proteins. Int. J. Syst. Bacteriol. 32:315–326
    [Google Scholar]
  11. Chatel D. L., Wise J. L., Marfleet A. G. 1979; Ryegrass toxicity organism found on other grasses. J. Agric. West. Aust. 20:89
    [Google Scholar]
  12. Cockrum P. A., Edgar J. A. 1983; High-performance liquid chromatographic comparison of the tunicaminyluracil-based antibiotic corynetoxin, tunicamycin, streptovirudin and MM 19290. J. Chromatogr. 268:245–254
    [Google Scholar]
  13. Collins M. D. 1983; Cell wall peptidoglycan and lipid composition of the phytopathogen Corynebacterium rathayi (Smith). Syst. Appl. Microbiol. 4:193–198
    [Google Scholar]
  14. Cummins C. S., Lelliott R. A., Keddie R. M. 1974 Corynebacterium. 602 Buchanan R. E., Gibbons N. E.ed Bergey’s manual of determinative bacteriology, 8th. The Williams & Wilkins Co.; Baltimore:
    [Google Scholar]
  15. Davis M. J., Gillaspie A. G. Jr., Vidaver A. K., Harris R. W. 1984; Clavibacter: a new genus containing some phytopathogenic coryneform bacteria, including Clavibacter xyli subsp. xyli sp. nov., subsp. nov. and Clavibacter xyli subsp. cynodontis subsp. nov., pathogens that cause ratoon stunting disease of sugarcane and bermudagrass stunting disease. Int. J. Syst. Bacteriol. 34:107–117
    [Google Scholar]
  16. Dye D. W., Kemp W. J. 1977; A taxonomic study of plant pathogenic Corynebacterium species. N.Z. J. Agric. Res. 20:563–582
    [Google Scholar]
  17. Edgar J. A., Frahn J. L., Cockrum P. A., Anderson N., Jago M. V., Culvenor C. C. J., Jones A. J., Murray K., Shaw K. J. 1982; Corynetoxins, causative agents of annual ryegrass toxicity: their identification as tunicamycin group antibiotics. J. Chern. Soc. Chem. Commun., 1982:222–224
    [Google Scholar]
  18. Fisher J. M. 1977; Annual ryegrass toxicity. Biennial report of the Waite Agricultural Research Institute. 1976-77:7–11
    [Google Scholar]
  19. Galloway J. H. 1961; Grass seed nematode poisoning in live-stock. J. Am. Vet. Med. Assoc. 139:1212–1214
    [Google Scholar]
  20. Gupta P., Swarup G. 1972; Ear-cockle and yellow ear-rot disease of wheat. II. Nematode bacterial association. Nematologia 18:320–324
    [Google Scholar]
  21. Harper J. J., Davis G. H. G. 1979; Two-dimensional thin-layer chromatography for amino acid analysis of bacterial cell walls. Int. J. Syst. Bacteriol. 29:56–58
    [Google Scholar]
  22. Lazar I. 1968; Serological relationships of Corynebacterium. J. Gen. Microbiol. 52:77–88
    [Google Scholar]
  23. Masuo E., Nakagawa T. 1970; Numerical classification of bacteria. IV. Relationships among some corynebacteria based on serological similarity alone. Agric. Biol. Chern. 34:13751382
    [Google Scholar]
  24. McLaughlin M. R., Barnett O. W., Burrows P. M., Baum R. H. 1981; Improved ELISA conditions for detection of plant viruses. J. Virol. Methods 3:13–25
    [Google Scholar]
  25. Moffett M. L., Fahy P. C., Cartwright D. 1983 Coryne-bacterium. 45–65 Fahy P. C., Persley G. J.ed Plant bacterial diseases; a diagnostic guide Academic Press; Sydney:
    [Google Scholar]
  26. Price P. C., Fisher J. M., Kerr A. 1979; Annual ryegrass toxicity: parasitism of Lolium rigidum by a seedgall forming nematode (Anguina sp.). Ann. Appl. Biol. 91:359–369
    [Google Scholar]
  27. Price P. C., Fisher J. M., Kerr A. 1979; On Anguina funesta n. sp., and its association with Corynebacterium sp., in infecting Lolium rigidum. Nematologica 25:76–85
    [Google Scholar]
  28. Sabet K. A. 1954; On the host range and systematic position of the bacteria responsible for the yellow slime disease of wheat (Triticum vulgare Vill.) and cocksfoot grass (Dactylis glomerata L.). Ann. Appl. Biol. 41:606–611
    [Google Scholar]
  29. Schleifer K. H., Kandler O. 1972; Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol. Rev. 36:407–477
    [Google Scholar]
  30. Severin V., Docea E. 1968; Contributii la studiul biologiei bacteriei Corynebacterium rathayi. Microbiologia 1:541–544
    [Google Scholar]
  31. Stynes B. A., Bird A. F. 1983; Development of annual ryegrass toxicity. Aust. J. Agric. Res. 34:653–660
    [Google Scholar]
  32. Stynes B. A., Petterson D. S., Lloyd J., Payne A. L., Lanigan G. W. 1979; The production of toxin in annual ryegrass, Lolium rigidum, infected with a nematode, Anguina sp., and Corynebacterium rathayi. Aust. J. Agric. Res. 30:201–209
    [Google Scholar]
  33. Stynes B. A., Wise J. L. 1980; The distribution and importance of annual ryegrass toxicity in Western Australia and its occurrence in relation to cropping rotations and cultural practices. Aust. J. Agric. Res. 31:557–569
    [Google Scholar]
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