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Comparisons of 5S ribosomal ribonucleic acid (rRNA) nucleotide sequences were used for the phylogenetic characterization of a number of “morphologically conspicuous” sulfide-oxidizing eubacteria and certain of their non-sulfide oxidizing counterparts. Nucleotide sequences were determined for 5S rRNAs isolated from Thiovulum sp., Beggiatoa alba B18LD, Thiothrix nivea JP2, strain 30 (a Thiothrix-like organism), Vitreoscilla beggiatoides B23SS, Vitreoscilla filiformis L1401-7, V.filiformis ATCC 15551, Vitreoscilla stercoraria VT1, and Leptothrix discophora (Stokes). All 5S rRNAs characterized in this study, except that of Thiovulum sp., belong to either the gamma or beta subdivisions of the “purple bacteria,” which form 1 of the 10 major eubacterial divisions so far defined by 16S rRNA oligonucleotide catalog analysis. Although its 5S rRNA is clearly eubacterial in character, Thiovulum sp. appears to have no specific relatives among the eubacteria presently represented in the 5S rRNA data collection.
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