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Criteria for description of new groups of the serogroup classification scheme for the Spiroplasma genus are proposed. New groups, if shown to be referable to the Mollicutes class and Spiroplasmataceae family, should be shown to differ from established groups by (i) reciprocal deformation tests and (ii) one of three other serologic tests (growth inhibition, metabolism inhibition, or enzyme-linked immunosorbent assay). Also, the ability or inability to utilize glucose, arginine, and urea should be determined. Determination of guanine-plus-cytosine content is strongly recommended but optional.
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