1887

Abstract

Fifty-two strains of species were isolated from oral cavities or lesions contaminated with feline oral flora from 49 different cats. Forty-five strains were from purulent lesions, while the remaining seven strains came from the normal gingivae of three cats less than 6 months old. Of 36 strains which were phenotypically like , 29 showed an average level of deoxyribonucleic acid (DNA) homology of 87% with the type strain of , strain ATCC 25533 (isolated from a cat). The remaining seven strains showed an average level of DNA relatedness to of 53%. An additional eight strains, which were identified as by biochemical tests, had polyacrylantide gel electrophoresis patterns unlike those of and an average level of DNA homology with ATCC 25286(T = type strain) of 25%. These strains showed an average level of DNA homology with ATCC 35896of 96%, although the results of polyacrylamide gel electrophoresis and biochemical tests obtained with the isolated strains were unlike the results obtained with ATCC 35896. Four strains isolated from cats had biochemical characteristics of ATCC 25586. The average level of intragroup DNA homology of these strains with ATCC 25586was 66%, although their polyacrylamide gel electrophoresis patterns differed somewhat from those of the type strain. Four strains isolated from cats were identified as on the basis of phenotypic tests; they had an average level of homology with the type strain of , strain ATCC 25832, of 17% and showed low degrees of relatedness with DNAs from all other type strains tested.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-37-1-23
1987-01-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/37/1/ijsem-37-1-23.html?itemId=/content/journal/ijsem/10.1099/00207713-37-1-23&mimeType=html&fmt=ahah

References

  1. Cato E. P., Moore L. V. H., Moore W. E. C. 1985; Fusobacterium alocis sp. nov. and Fusobacterium sulci sp. nov. from the human gingival sulcus. Int. J. Syst. Bacteriol. 35:475–477
    [Google Scholar]
  2. Holdeman L. V., Cato E. P., Moore W. E. C.ed 1977 Anaerobe laboratory manual. , 4th. Virginia Polytechnic Institute and State University; Blacksburg:
    [Google Scholar]
  3. Johnson J. L. 1981 Genetic characterization. 450–472 Gerhardt P., Murray R. G. E., Costilow R. N., Nester E. W., Wood W. A., Krieg N. R., Phillips G. B.ed Manual of methods for general bacteriology American Society for Microbiology; Washington, D.C.:
    [Google Scholar]
  4. Love D. N., Johnson J. L., Jones R. F., Bailey M. 1985; Comparison of Bacteroides zoogleoformans strains isolated from soft tissue infections in cats with strains from periodontal disease in humans. Infect. Immun. 47:166–168
    [Google Scholar]
  5. Love D. N., Jones R. F., Bailey M. 1980; Characterization of Fusobacterium species isolated from soft tissue infections in cats. J. Appl. Bacteriol. 48:325–331
    [Google Scholar]
  6. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J. Mol. Biol. 3:208–218
    [Google Scholar]
  7. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J. Mol. Biol. 5:109–118
    [Google Scholar]
  8. Moore W. E. C., Hash D. E., Holdeman L. V., Cato E. P. 1980; Polyacrylamide slab gel electrophoresis of soluble proteins for studies of bacterial floras. Appl. Environ. Microbiol. 39:900–907
    [Google Scholar]
  9. Moore W. E. C., Holdeman L. V., Kelley R. W. 1984 Genus 11. Fusobacterium Knorr 1922, 4AL. 631–637 Krieg N. R., Holt J. G.ed Bergey’s manual of systematic bacteriology 1 The Williams & Wilkins Co.; Baltimore:
    [Google Scholar]
  10. Selin Y. M., Harich B., Johnson J. L. 1983; Preparation of labeled nucleic acids (nick translation and iodination) for DNA homology and rRNA hybridization experiments. Curr. Microbiol. 8:127–132
    [Google Scholar]
  11. Slots J., Potts T. V. 1982; Fusobacterium simiae, a new species from monkey dental plaque. Int. J. Syst. Bacteriol. 32:191–194
    [Google Scholar]
  12. Slots J., Potts T. V. 1983; Fusobacterium simiae, a new species from monkey dental plaque. Int. J. Syst. Bacteriol. 33:442
    [Google Scholar]
  13. Tereba A., McCarthy B. J. 1973; Hybridization of 125I labelled ribonucleic acid. Biochemistry 12:4675–4679
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-37-1-23
Loading
/content/journal/ijsem/10.1099/00207713-37-1-23
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error