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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 36, Issue 4

DNA Homologies between , Rhizobia That Nodulate sp., and Other and Species Free

Abstract

The relationships between and the rhizobia that nodulate and (goat’s rue) and recognized species of and were investigated by using deoxyribonucleic acid (DNA)-DNA hybridization, legume nodulation tests, and phage typing. The strains formed a distinct DNA homology group which could be divided into two subgroups. The mean levels of relative homology at 65°C of 11 strains of with reference strains USDA 208 and USDA 191 were 86 and 80%, respectively. These values were significantly higher (Student’s test, < 0.001) than the mean levels of relative homology with DNAs from other groups of rhizobia. The -nodulating rhizobia also formed a distinct DNA homology group. The mean levels of relative homology at 65°C of DNAs from 11 strains with DNAs from reference strains gall and galNW3, which effectively nodulate , were 79 and 85%, respectively. These values were also significantly higher (Student’s test, < 0.001) than the values obtained with DNAs from other groups of rhizobia. These results correlated with cross-inoculation and phage-typing results and indicated that the two groups are genetically distinct. Genomic DNA was used as a probe in a modified colony hybridization autoradiographic procedure for the identification of DNAs from , and sp. () in colonies and from nodules.

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Copyright © 1986 International Union of Microbiological Societies
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1986-10-01
2025-01-18
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/content/journal/ijsem/10.1099/00207713-36-4-550
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DNA Homologies between Rhizobium fredii, Rhizobia That Nodulate Galega sp., and Other Rhizobium and Bradyrhizobium Species
Int J Syst Evol Microbiol 36, 550 (1986); https://doi.org/10.1099/00207713-36-4-550
/content/journal/ijsem/10.1099/00207713-36-4-550
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