1887

Abstract

The genome sizes of the type strains of the sterol-requiring organisms and and of a sterol-nonrequiring obligately anaerobic mollicute, strain 161, were determined by the renaturation method of Wetmur and Davidson. The genome molecular weights of the three strains were almost identical (viz., approximately 10). This value is identical to the genome molecular weights of members of the family in the order and of the family in the order . The guanine-plus-cytosine contents of the deoxyribonucleic acids of , and strain 161, as determined by the buoyant density method, were found to be 30.1, 32.8, and 39.9 mol%, respectively, thereby confirming results reported previously by other workers. The deoxyribonucleic acid base composition and genomic size are among the data needed to clarify the taxonomic status of the genus and of the sterol-nonrequiring anaerobic organism strain 161.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-36-3-483
1986-07-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/36/3/ijsem-36-3-483.html?itemId=/content/journal/ijsem/10.1099/00207713-36-3-483&mimeType=html&fmt=ahah

References

  1. Bak A. L., Black F. T., Christiansen C., Freundt E. A. 1969; Genome size of mycoplasmal DNA. Nature (London) 224:1209–1210
    [Google Scholar]
  2. Bryant M. P., Burkey L. A. 1953; Cultural methods and some characteristics of some of the numerous groups of bacteria in the bovine rumen. J. Dairy Sci. 36:205–217
    [Google Scholar]
  3. Christiansen C., Black F. T., Freundt E. A. 1981; Hybridization experiments with deoxyribonucleic acid from Ureaplasma urealyticum serovars I to VIII. Int. J. Syst. Bacteriol. 31:259–262
    [Google Scholar]
  4. Christiansen C., Christiansen G., Bak A. L. 1974; Heterogeneity of mitochondrial DNA from Saccharomyces carlsbergensis: renaturation and sedimentation studies. J. Mol. Biol. 84:65–82
    [Google Scholar]
  5. Hungate R. E. 1969 A roll tube method for cultivation of strict anaerobes. 117–132 Norris J. R., Ribbons D. N.ed Methods in microbiology 3B Academic Press, Inc.; New York:
    [Google Scholar]
  6. International Committee on Systematic Bacteriology Subcommittee on the Taxonomy of Mollicutes 1979; Proposal of minimal standards for descriptions of new species of the class Mollicutes. Int. J. Syst. Bacteriol. 29:172–180
    [Google Scholar]
  7. International Committee on Systematic Bacteriology Subcommittee on the Taxonomy of Mycoplasmatales 1977Minutes of interim meeting22 September 1976London, United Kingdom Int. J. Syst. Bacteriol. 27392–394
    [Google Scholar]
  8. Razin S., Freundt E. A. 1984 Division Tenericutes div. nov. (q.v.p. 36) class I. Mollicutes Edward and Freundt 1967, 267AL. 741 Krieg N. R., Holt J. G.ed Bergey’s manual of systematic bacteriology 1 The Williams & Wilkins Co.; Baltimore:
    [Google Scholar]
  9. Robinson I. M. 1983 Culture media for anaeroplasmas. 159–162 Razin S., Tully J. G.ed Methods in mycoplasmology 1 Academic Press, Inc.; New York:
    [Google Scholar]
  10. Robinson I. M. 1984 Genus Anaeroplasma Robinson, Allison and Hartman 1975, 173AL. 787–790 Krieg N. R., Holt J. G.ed Bergey’s manual of systematic bacteriology 1 The Williams & Wilkins Co.; Baltimore:
    [Google Scholar]
  11. Robinson I. M., Allison M. J. 1975; Transfer of Acholeplasma bactoclasticum Robinson and Hungate to the genus Anaeroplasma (Anaeroplasma bactoclasticum [Robinson and Hungate] comb, nov.): emended description of the species. Int. J. Syst. Bacteriol. 25:182–186
    [Google Scholar]
  12. Robinson I. M., Allison M. J., Hartman P. A. 1975; Anaeroplasma abactoclasticum gen.nov., sp.nov.: an obligately anaerobic mycoplasma from the rumen. Int. J. Syst. Bacteriol. 25:173–181
    [Google Scholar]
  13. Robinson I. M., Rhoades K. R. 1977; Serological relationships between strains of anaerobic mycoplasmas. Int. J. Syst. Bacteriol. 27:200–203
    [Google Scholar]
  14. Schildkraut C. L., Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its buoyant density in CsCl. J. Mol. Biol. 4:430–443
    [Google Scholar]
  15. Stephens E. B., Robinson I. M., Barile M. F. 1985; Nucleic acid relationships among the anaerobic mycoplasmas. J. Gen. Microbiol. 131:1223–1227
    [Google Scholar]
  16. Wetmur J. G., Davidson N. 1968; Kinetics of renaturaion of DNA. J. Mol. Biol. 31:349–370
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-36-3-483
Loading
/content/journal/ijsem/10.1099/00207713-36-3-483
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error