1887

Abstract

The lobster pathogen and other gram-positive, marine -like cocci were examined morphologically, biochemically, and genetically. Morphologically, the lobster pathogenic strains were unique in their tetrad-forming capacity. Because of intragroup fermentative variations among the lobster-pathogenic strains and values of deoxyribonucleic acid (DNA) base composition overlapping those of the other cocci, the lobster pathogens did not stand out as a separate group based on these data. According to the DNA-DNA hybridization studies, however, the lobster-pathogenic strains were very closely related (80 to 100% homology) and could be easily separated from the remaining strains. All the cocci had extraordinarily small genomes ranging from 0.57 x 10 to 1.01 x 10 daltons.

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1986-07-01
2024-12-07
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References

  1. Bak A. L., Christiansen C., Stenderup A. 1970; Bacterial genome sizes determined by DNA renaturation studies. J. Gen. Microbiol. 64:377–380
    [Google Scholar]
  2. Cairns J. 1963; The chromosome of Escherichia coli. Cold Spring Harbor Symp. Quant. Biol. 28:43–46
    [Google Scholar]
  3. Collins C. H. 1964 Microbiological methods. 84 Butterworth & Co., Ltd.; London:
    [Google Scholar]
  4. Conn H. J., Bartholomew J. W., Jennisson M. W. 1954 Leaflet IV, Staining methods. 7 Committee on Bacteriological Technic of the Society of American Bacteriologistsed Manual of methods for pure culture study of bacteria Biotech Publications; New York:
    [Google Scholar]
  5. Deibel R. H., Niven C. F. Jr. 1960; Comparative study of Gaffkya homari, Aerococcus viridans, tetrad-forming cocci from meat curing brines, and the genus Pediococcus. J. Bacteriol. 79:175–180
    [Google Scholar]
  6. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur. J. Biochem. 12:133–142
    [Google Scholar]
  7. Egidius E. 1972; On the internal bacterial flora of the European lobster (Homarus vulgaris) and its susceptibility of gaffkemia. Aquaculture 1:193–197
    [Google Scholar]
  8. Evans J. B. 1974 Genus IV. Aerococcus Williams, Hirch and Cowan 1953, 475. 515–516 Buchanan R. E., Gibbons N. E.ed Bergey’s manual of determinative bacteriology, 8th ed.. The Williams & Wilkins Co.; Baltimore:
    [Google Scholar]
  9. Gillis M., De Ley J. 1975; Determination of the molecular complexity of double-stranded phage genome DNA from initial renaturation rates: the effect of DNA base composition. J. Mol. Biol. 98:447–464
    [Google Scholar]
  10. Gillis M., De Ley J., De Cleene M. 1970; The determination of molecular weight of bacterial genome DNA from renaturation rates. Eur. J. Biochem. 12:143–153
    [Google Scholar]
  11. Heuch I. 1982 Syllabus of the subject M 152: applied statistics. Institute of Mathematics, University of Bergen; Norway:
    [Google Scholar]
  12. Hitchner E. R., Snieszko S. F. 1947; A study of a microorganism causing a bacterial disease of lobsters. J. Bacteriol. 54:58
    [Google Scholar]
  13. Huss V. A. R., Festl H., Schleiter K. H. 1983; Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst. Appl. Microbiol. 4:184–192
    [Google Scholar]
  14. Kelly K. F., Evans J. B. 1974; Deoxyribonucleic acid homology among strains of the lobster pathogen “ Gaffkya homari” and Aerococcus viridans. J. Gen. Microbiol. 81:257–260
    [Google Scholar]
  15. Mandel M., Marmur J. 1968; Use of ultraviolet absorbance-temperature profile for determining the guanine plus cytosine content of DNA. Methods Enzymol. 12:195–206
    [Google Scholar]
  16. Marmur J. 1963; A procedure for the isolation of deoxyribonucleic acid from microorganisms. Methods Enzymol. 6:726–738
    [Google Scholar]
  17. Razin S., Freundt E. A. 1984 Class I. Mollicutes Edward and Freundt 1967, 267AL. 740–741 Krieg N. R., Holt J. G.ed Bergey’s manual of systematic bacteriology 1 The Williams & Wilkins Co.; Baltimore:
    [Google Scholar]
  18. Schultes L. M. 1969 Deoxyribonucleic acid homologies among aerococci and related bacteria. University Microfilms International; Ann Arbor, Mich:
    [Google Scholar]
  19. Schultes L. M., Evans J. B. 1971; Deoxyribonucleic acid homology of Aerococcus viridans. Int. J. Syst. Bacteriol. 21:207–209
    [Google Scholar]
  20. Steenbergen J. F., Kimball H. S., Low D. A., Schapiro H. C., Phelps L. N. 1977; Serological grouping of virulent and avirulent strains of the lobster pathogen Aerococcus viridans. J. Gen. Microbiol. 99:425–430
    [Google Scholar]
  21. Stewart J. E., Cornick J. W., Spears D. I. 1966; Incidence of Gaffkya homari in natural lobster (Homarus americanus) populations of the Atlantic region of Canada. J. Fish. Res. Board Can. 23:1325–1330
    [Google Scholar]
  22. Williams R. E. O., Hirch A., Cowan S. T. 1953; Aerococcus, a new bacterial genus. J. Gen. Microbiol. 8:475–480
    [Google Scholar]
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