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Five strains of actinomyces isolated from dental plaque of cattle formed a homogeneous group on the basis of Gram staining, colonial and cellular morphology, fermentation reactions, acid end products of metabolism, sodium dodecyl sulfate-polyacrylamide gel electrophoresis patterns of polypeptides, cell wall carbohydrates, and peptidoglycan and deoxyribonucleic acid mean base compositions. These organisms were closely related, as determined by deoxyribonucleic acid-deoxyribonucleic acid homology, which distinguishes them from strains of Actinomyces naeslundii, Actinomyces viscosus, Actinomyces denticolens, and Actinomyces howellii. A new taxon, Actinomyces slackii, is proposed for these isolates. The type strains of A. slackii is strain NCTC 11923.
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