1887

Abstract

Strains of serogroup 4 and and three strains of a new species, for which the name is proposed, were isolated from an oxidation pond in Israel. exhibited the following biochemical reactions typical of legionellae: Growth on buffered cysteine-yeast extract agar but not on chocolate agar or on buffered yeast extract agar without cysteine; nitrate negative; urease negative; nonfermentative; catalase positive; and motile. It did not hydrolyze hippurate, autofluoresce, or produce a brown pigment on tyrosine-containing media, and it had weakly positive gelatinase activity. It had predominantly branched-chain cellular fatty acids, similar to those described for other legionellae, and had major amounts of ubiquinones with more than 10 isoprene units in their side chains. It could be distinguished from all previously described species and serogroups by using antisera in slide agglutination tests. As determined by the hydroxyapatite method at 60

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1986-07-01
2024-12-02
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References

  1. Bercovier H., Goodman A., Israeli A., Shapiro M. 1984; Isolation of Legionella pneumophila serogroup 1 from a fatal case of pneumonia in Israel. Isr. J. Med. Sci. 20:1109–1111
    [Google Scholar]
  2. Boldur I., Ergaz M., Sompolinsky D. 1984; The first isolation of Legionella pneumophila in Israel. Isr. J. Med. Sci. 20:74–75
    [Google Scholar]
  3. Bopp C. A., Sumner J. W., Morris G. K., Wells J. G. 1981; Isolation of Legionella from environmental water samples by low pH treatment and use of selective medium. J. Clin. Microbiol. 13:714–719
    [Google Scholar]
  4. Brenner D. J., Steigerwalt A. G., Gorman G. W., Wilkinson H. W., Bibb W. F., Hackel M., Tyndall R. L., Campbell J., Feeley J. C., Thacker W. L., Skaliy P., Martin W. T., Brake B. J., Fields B. S., McEachern H. V., Corcoran L. K. 1985; Ten new species of Legionella. Int. J. Syst. Bacteriol. 35:50–59
    [Google Scholar]
  5. Brenner D. J., Steigerwalt A. G., McDade J. E. 1979; Classification of the Legionnaires’ disease bacterium: Legionella pneumophila genus novum, species nova of the family Legionellaçeae, familia nova. Ann. Intern. Med. 90:656–658
    [Google Scholar]
  6. Edelstein P. H. 1981; Improved semiselective medium for isolation of Legionella pneumophila from contaminated clinical and environmental specimens. J. Clin. Microbiol. 14:298–303
    [Google Scholar]
  7. Fliermans C. B., Cherry W. B., Orrison L. H., Smith S. J., Tison D. L., Pope D. H. 1981; Ecological distribution of Legionella pneumophila. Appl. Environ. Microbiol. 41:9–16
    [Google Scholar]
  8. Fraser D. W., Tsai T. F., Orenstein W., Parkin W. E., Beecham H. J., Sharrer R. G., Harris J., Mallison G. F., Martin S. M., McDade J. E., Shepard C. C., Brachman P. S.the Field Investigation Team 1977; Legionnaires’ disease: description of an epidemic. N. Engl. J. Med. 297:1189–1197
    [Google Scholar]
  9. Hébert G. A., Moss C. W., McDougal L. K., Bozeman F. M., McKinney R. M., Brenner D. J. 1980; The rickettsia-like organisms TATLOCK (1943) and HEBA (1959) as bacteria phenotypically similar to, but genetically distinct from Legionella pneumophila and the WIGA bacterium. Ann. Intern. Med. 92:45–52
    [Google Scholar]
  10. Karr D. E., Bibb W. F., Moss C. W. 1982; Isoprenoid quinones of the genus Legionella. J. Clin. Microbiol. 15:1044–1048
    [Google Scholar]
  11. Kaufmann A. F., McDade J. E., Patton C. M., Bennett J. V., Skaliy P., Feeley J. C., Anderson D. C., Potter M. E., Newhouse V. F., Gregg M. B., Brachman P. S. 1981; Pontiac fever: isolation of the etiologic agent (Legionella pneumophila) and demonstration of its mode of transmission. Am. J. Epidemiol. 114:337–347
    [Google Scholar]
  12. Kohne D. E., Steigerwalt A. G., Brenner D. J. 1984 Nucleic acid probe specific for members of the genus Legionella. 107–108 Thomsberry C., Balows A., Feeley J. C., Jakubowski W.ed Legionella. Proceedings of the Second International Symposium American Society for Microbiology; Washington, D.C:
    [Google Scholar]
  13. McDade J. E., Brenner D. J., Bozeman F. M. 1979; Legionnaires’ disease bacterium isolated in 1947. Ann. Intern. Med. 90:659–661
    [Google Scholar]
  14. McDade J. E., Shepard C. C., Fraser D. W., Tsai T. R., Redus M. A., Dowdle W. R.the Laboratory Investigation Team 1977; Legionnaires’ disease: isolation of a bacterium and demonstration of its role in other respiratory disease. N. Engl. J. Med. 297:1197–1203
    [Google Scholar]
  15. McKinney R. M., Thacker L., Harris P. P., Lewallen K. R., Hébert G. A., Edelstein P. H., Thomason B. M. 1979; Four serogroups of Legionnaires’ disease bacteria defined by immunofluorescence. Ann. Intern. Med. 90:621–624
    [Google Scholar]
  16. Meenhorst P. L., Reingold A. L., Groothius D. G., Gorman G. W., Wilkinson H. W., McKinney R. M., Feeley J. C., Brenner D. J., van Furth R. 1985; Water-related nosocomial pneumonia caused by Legionella pneumophila serogroups 1 and 10. J. Infect. Dis. 152:356–364
    [Google Scholar]
  17. Moss C. W., Bibb W. F., Karr D. E., Guerrant G. O. 1983; Chemical analysis of the genus Legionella: fatty acids and isoprenoid quinones. INSERM 114:375–380
    [Google Scholar]
  18. Moss C. W., Bibb W. F., Karr D. E., Guerrant G. O., Lambert M. A. 1983; Cellular fatty acid composition and ubiquinone content of Legionella feeleii sp. nov. J. Clin. Microbiol. 18:917–919
    [Google Scholar]
  19. Moss C. W., Guerrant G. O. 1983; Separation of bacterial ubiquinones using reverse-phase high-performance liquid chromatography. J. Clin. Microbiol. 18:15–17
    [Google Scholar]
  20. Moss C. W., Weaver R. E., Dees S. B., Cherry W. B. 1977; Cellular fatty acid composition of isolates from Legionnaires’ disease. J. Clin. Microbiol. 6:140–143
    [Google Scholar]
  21. Orrison L. H., Cherry W. B., Milan D. 1981; Isolation of Legionella from cooling tower water by filtration. Appl. Environ. Microbiol. 41:1202–1205
    [Google Scholar]
  22. Tang P. W., Toma S., MacMillan L. G. 1985; Legionella oakridgensis: laboratory diagnosis of a human infection. J. Clin. Microbiol. 21:462–463
    [Google Scholar]
  23. Thacker W. L., Plikaytis B. B., Wilkinson H. W. 1985; Identification of 22 Legionella species and 33 serogroups with the slide agglutination test. J. Clin. Microbiol. 21:779–782
    [Google Scholar]
  24. Thacker W. L., Wilkinson H. W., Plikaytis B. B., Steigerwalt A. G., Mayberry W. R., Moss C. W., Brenner D. J. 1985; Second serogroup of Legionella feeleii strains isolated from humans. J. Clin. Microbiol. 22:1–4
    [Google Scholar]
  25. Thorpe T. C., Miller R. D. 1980; Negative enrichment procedure for isolation of Legionella pneumophila from seeded cooling tower water. Appl. Environ. Microbiol. 40:849–851
    [Google Scholar]
  26. Tobin J. O’H., Swann R. A., Bartlett C. L. R. 1981; Isolation of Legionella pneumophila from water systems: methods and preliminary results. Br. Med. J. 282:515–517
    [Google Scholar]
  27. Wadowsky R. M., Yee R. B. 1981; A glycine-containing selective medium for isolation of Legionellaceae from environmental specimens. Appl. Environ. Microbiol. 42:768–772
    [Google Scholar]
  28. Wilkinson H. W., Thacker W. L., Brenner D. J., Ryan K. 1985; Fatal Legionella maceachernii pneumonia. J. Clin. Microbiol. 22:1055
    [Google Scholar]
  29. Wilkinson H. W., Thacker W. L., Steigerwalt A. G., Brenner D. J., Ampel N. M., Wing E. J. 1985; Second serogroup of Legionella hackeliae isolated from a patient with pneumonia. J. Clin. Microbiol. 22:488–489
    [Google Scholar]
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